Retention behavior of carboxylic acids and their quaternary ammonium ion pairs in reversed phase chromatography with acetonitrile as organic modifier in the mobile phase

1979 ◽  
Vol 51 (7) ◽  
pp. 976-983 ◽  
Author(s):  
A. Tilly-Melin ◽  
Y. Askemark ◽  
K. G. Wahlund ◽  
Goran. Schill
Keyword(s):  
Carboxylic Acids ◽  
Quaternary Ammonium ◽  
Mobile Phase ◽  
Ion Pairs ◽  
Reversed Phase ◽  
Ammonium Ion ◽  
Organic Modifier ◽  
Retention Behavior ◽  
Reversed Phase Chromatography

Mobile phase effects in reversed-phase chromatography

1979 ◽  
Vol 185 ◽  
pp. 129-152 ◽  
Author(s):  
Wayne R. Melander ◽  
Avi Nahum ◽  
Csaba Horváth
Keyword(s):  
Mobile Phase ◽  
Reversed Phase ◽  
Reversed Phase Chromatography ◽  
Mobile Phase Effects

Application of Mobile Phases Containing Ionic Liquid for HPLC Analysis of Selected Isoquinoline Alkaloids

2017 ◽  
Vol 100 (6) ◽  
pp. 1652-1659 ◽  
Author(s):  
Anna Petruczynik ◽  
Justyna Misiurek ◽  
Tomasz Tuzimski ◽  
Monika Waksmundzka-Hajnos
Keyword(s):  
Ionic Liquid ◽  
Stationary Phases ◽  
Reversed Phase ◽  
System Efficiency ◽  
Organic Modifier ◽  
Optimal Conditions ◽  
Retention Behavior ◽  
Isoquinoline Alkaloids ◽  
Mobile Phases ◽  
Chemical Groups

Abstract An HPLC procedure on a polar reversed-phase column with mobile phases containing ionic liquid (IL) was developed for the analysis of selected alkaloids from different chemical groups. We aimed to obtain optimal conditions for the separation of alkaloids because widely used silica-based stationary phases exhibit a silanol effect, rendering analysis of basic analytes extremely difficult. Retention, separation selectivity, peak symmetry, and system efficiency were examined in various eluent systems containing different concentrations of IL and acetonitrile. The obtained results revealed substantial influence from the concentrations of IL, the organic modifier, and temperature on the retention behavior of the investigated alkaloids. The most selective and efficient chromatographic systems were applied for the analysis of several alkaloids in a plant extract.

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