Retention behavior of some monobasic penicillins and their quaternary ammonium ion pairs in reversed-phase high-performance liquid chromatography

1991 ◽  
Vol 32 (9-10) ◽  
pp. 436-440 ◽  
Author(s):  
D. J. Fletouris ◽  
J. E. Psomas ◽  
A. J. Mantis
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Quaternary Ammonium ◽  
High Performance ◽  
Ion Pairs ◽  
Reversed Phase ◽  
Ammonium Ion ◽  
Retention Behavior

Direct determination of four sulfates and seven glucuronides of 17-oxosteroids in urine by fluorescence "high-performance" liquid chromatography.

1989 ◽  
Vol 35 (5) ◽  
pp. 794-799 ◽  
Author(s):  
J Iwata ◽  
T Suga
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Direct Method ◽  
Ion Pairs ◽  
Direct Determination ◽  
Reversed Phase ◽  
Urine Samples ◽  
Acetonitrile Solution ◽  
Fluorometric Detection

Abstract We describe a direct method for determining four sulfates and seven glucuronides of 17-oxosteroids (17OS) in urine without hydrolysis, by use of "high-performance" liquid chromatography (HPLC) with fluorometric detection. After pretreatment of urine samples with a Sep-Pak C18 cartridge, four 17OS sulfates and seven 17OS glucuronides in the pretreated urine samples were reacted with tetrapentylammonium ions to form ion pairs. Ion-paired 17OS sulfates were extracted with benzene. By adding sodium sulfate to the remaining sample, we could then extract ion-paired 17OS glucuronides with dichloromethane. Each extract was labeled with dansyl-hydrazine in an acetic acid-acetonitrile solution. The labeled steroids were separated by HPLC on a reversed-phase Capcell-Pak C8 (silicon-polymer-coated silica gel modified with octyl groups). We monitored each effluent with a fluorometric detector (330 nmexcitation, 535 nmemission).

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