Determination of the aqueous solubility of polynuclear aromatic hydrocarbons by a coupled column liquid chromatographic technique

1978 ◽  
Vol 50 (1) ◽  
pp. 175-179 ◽  
Author(s):  
Willie E. May ◽  
Stanley P. Wasik ◽  
David H. Freeman
Keyword(s):  
Aromatic Hydrocarbons ◽  
Aqueous Solubility ◽  
Polynuclear Aromatic Hydrocarbons ◽  
Chromatographic Technique ◽  
Column Liquid Chromatographic ◽  
Liquid Chromatographic

High Pressure Liquid Chromatographic Method for Determination of Polynuclear Aromatic Hydrocarbons in Beer

1981 ◽  
Vol 64 (3) ◽  
pp. 641-646
Author(s):  
Frank L Joe ◽  
Emma L Roseboro ◽  
Thomas Fazio
Keyword(s):  
High Pressure ◽  
Aromatic Hydrocarbons ◽  
Hplc Method ◽  
Polynuclear Aromatic Hydrocarbons ◽  
Fluorescence Measurement ◽  
High Pressure Liquid Chromatographic ◽  
Neutral Alumina ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Abstract A high pressure liquid chromatographic (HPLC) method has been developed for the determination of some polynuclear aromatic hydrocarbons (PAHs) in beer. The PAHs are extracted into isooctane from a beer sample to which sodium hydroxide, ethanol, and acetone have been added. The isooctane extract is washed with acid and then base, and is purified by chromatography through deactivated neutral alumina. The eluate from the column is concentrated to dryness and the resulting residue is dissolved in methanol-acetonitrile (1 + 1) and subjected to reverse phase HPLC analysis. Both ultraviolet (UV) and fluorescence detectors are used to monitor the HPLC column effluent. Recovery of 4 PAHs, benz(a)anthracene, benzo(a)pyrene, benzo(g,h,i)perylene, and dibenzo(a,i)pyrene, ranged from 77 to 108% by UV measurement and 73 to 97% by fluorescence measurement.

Liquid Chromatographic Determination of Trace Residues of Polynuclear Aromatic Hydrocarbons in Smoked Foods

1984 ◽  
Vol 67 (6) ◽  
pp. 1076-1082 ◽  
Author(s):  
Frank L Joe ◽  
Jean Salemme ◽  
Thomas Fazio
Keyword(s):  
Dimethyl Sulfoxide ◽  
Aromatic Hydrocarbons ◽  
Chromatographic Determination ◽  
Polynuclear Aromatic Hydrocarbons ◽  
Alumina Column ◽  
Uv Detectors ◽  
Market Basket ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A liquid chromatographic (LC) method was developed and applied to the determination of polynuclear aromatic hydrocarbons (PAHs) in a variety of smoked, market basket commodities. The PAHs are extracted with l,l,2-trichIoro-l,2,2-trifluoroethane (Freon 113) from alcoholic KOH digests of the commodities. The extracts are purified by column chromatography through a deactivated silica gel/alumina column and by liquid-liquid partitioning between dimethyl sulfoxide and cyclohexane before separation of the PAHs by LC. Both fluorescence and UV detectors are used to monitor the LC column effluent to detect nanogram quantities of PAHs. Trace levels of carcinogenic and noncarcinogenic PAHs were found in all samples analyzed, although generally at <1 ppb levels.

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