Liquid Chromatographic Determination of Nitro-Substituted Polynuclear Aromatic Hydrocarbons by Sequential Electrochemical and Fluorescence Detection

1996 ◽  
Vol 68 (7) ◽  
pp. 1226-1232 ◽  
Author(s):  
Mitsunori Murayama ◽  
Purnendu K. Dasgupta
Keyword(s):  
Fluorescence Detection ◽  
Aromatic Hydrocarbons ◽  
Chromatographic Determination ◽  
Polynuclear Aromatic Hydrocarbons ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Liquid Chromatographic Determination of Trace Residues of Polynuclear Aromatic Hydrocarbons in Smoked Foods

1984 ◽  
Vol 67 (6) ◽  
pp. 1076-1082 ◽  
Author(s):  
Frank L Joe ◽  
Jean Salemme ◽  
Thomas Fazio
Keyword(s):  
Dimethyl Sulfoxide ◽  
Aromatic Hydrocarbons ◽  
Chromatographic Determination ◽  
Polynuclear Aromatic Hydrocarbons ◽  
Alumina Column ◽  
Uv Detectors ◽  
Market Basket ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A liquid chromatographic (LC) method was developed and applied to the determination of polynuclear aromatic hydrocarbons (PAHs) in a variety of smoked, market basket commodities. The PAHs are extracted with l,l,2-trichIoro-l,2,2-trifluoroethane (Freon 113) from alcoholic KOH digests of the commodities. The extracts are purified by column chromatography through a deactivated silica gel/alumina column and by liquid-liquid partitioning between dimethyl sulfoxide and cyclohexane before separation of the PAHs by LC. Both fluorescence and UV detectors are used to monitor the LC column effluent to detect nanogram quantities of PAHs. Trace levels of carcinogenic and noncarcinogenic PAHs were found in all samples analyzed, although generally at <1 ppb levels.

Liquid Chromatographic Determination of Zearalenone and Zearalenol in Animal Feeds and Grains, Using Fluorescence Detection

1986 ◽  
Vol 69 (5) ◽  
pp. 894-898 ◽  
Author(s):  
Renee W Bagneris ◽  
Jean A Gaul ◽  
George M Ware
Keyword(s):  
Fluorescence Detection ◽  
Limit Of Detection ◽  
Chromatographic Determination ◽  
Excitation Wavelength ◽  
Animal Feeds ◽  
Coefficients Of Variation ◽  
Liquid Chromatographic Determination ◽  
Sorghum Silage ◽  
Liquid Chromatographic

Abstract A liquid chromatographic (LC) method was developed for the determination of zearalenone and zearalenol in grains and mixed animal feeds. Samples are extracted with chloroform and purified by a baseacid liquid-liquid partition. Zearalenone and zearalenol are separated by reverse phase LC and determined by fluorescence detection, excitation wavelength 236 nm with a 418 nm cutoff filter. The method was applied to the determination of zearalenone and zearalenol in 395 survey samples of corn, oats, barley, sorghum, silage, and finished feeds. The limit of detection is 10 ng/g for both toxins. The range of naturally occurring toxins found was 10-4000 ng/g. Average recoveries were 84% for zearalenone and 69% for zearalenol. Coefficients of variation were 24.6% for zearalenone and 30.8% for zearalenol for crop year 1980, and 28.3% for zearalenone and 22.0% for zearalenol for crop year 1981.

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