Laser two-photon excited fluorescence detection for high pressure liquid chromatography

1977 ◽  
Vol 49 (11) ◽  
pp. 1554-1556 ◽  
Author(s):  
Michael J. Sepaniak ◽  
Edward S. Yeung
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Fluorescence Detection ◽  
Two Photon

Determination of Zearalenone in Corn by High Pressure Liquid Chromatography and Fluorescence Detection

1978 ◽  
Vol 61 (5) ◽  
pp. 1058-1062
Author(s):  
George M Ware ◽  
Charles W Thorpe
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Fluorescence Detection ◽  
Hplc Analysis ◽  
Fluorescence Detector ◽  
Reverse Phase

Abstract A method is reported for determining zearalenone in corn at levels as low as 10 ng/g. Samples are extracted with chloroform-water and cleaned up by liquid-liquid chromatography, and the zearalenone is detected by a fluorescence detector after separation by reverse phase high pressure liquid chromatography (HPLC). Recoveries of zearalenone added to corn at levels from 10 to 200 ng/g averaged greater than 89%. In addition, a confirmation procedure is described which involves sequential HPLC analysis of the sample and a zearalenone standard, using 4 different excitation wavelengths and comparing fluorescence responses obtained. This method was successfully applied to the analysis of 11 samples of cornmeal; zearalenone was detected in 9 of the samples at levels from 11 to 69 ng/g.

Residues of glyphosate and its metabolite AMPA in strawberry fruit following spot and wiper applications

1992 ◽  
Vol 72 (4) ◽  
pp. 1359-1365 ◽  
Author(s):  
Allan J. Cessna ◽  
Nancy P. Cain
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Key Words ◽  
High Pressure Liquid Chromatography ◽  
Fluorescence Detection ◽  
Major Metabolite ◽  
Strawberry Fruit ◽  
Fruit Tissue ◽  
Aminomethylphosphonic Acid

In a 3-yr study, residues of glyphosate and its major metabolite aminomethylphosphonic acid (AMPA) were determined, using high-pressure liquid chromatography with post-column reaction and fluorescence detection, in strawberry fruit following spot and wiper applications of glyphosate to established strawberries. Residues in the fruit were not significantly different for the wiper and spot treatments. Maximum residues of glyphosate detected for either treatment were of the order of 0.04 mg kg−1; residues of AMPA were non-detectable in all samples. Recoveries of glyphosate and AMPA from fortified strawberry fruit tissue were 76 ± 8% and 79 ± 13%, respectively, at 0.1 mg kg−1.Key words: Glyphosate, AMPA, strawberry, wiper application, spot application, residues, HPLC

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