Determination of aflatoxin M1 by high-pressure liquid chromatography using fluorescence detection

1980 ◽  
Vol 28 (2) ◽  
pp. 481-482 ◽  
Author(s):  
Roberta M. Beebe ◽  
David M. Takahashi
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Fluorescence Detection ◽  
Aflatoxin M1

Determination of Zearalenone in Corn by High Pressure Liquid Chromatography and Fluorescence Detection

1978 ◽  
Vol 61 (5) ◽  
pp. 1058-1062
Author(s):  
George M Ware ◽  
Charles W Thorpe
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Fluorescence Detection ◽  
Hplc Analysis ◽  
Fluorescence Detector ◽  
Reverse Phase

Abstract A method is reported for determining zearalenone in corn at levels as low as 10 ng/g. Samples are extracted with chloroform-water and cleaned up by liquid-liquid chromatography, and the zearalenone is detected by a fluorescence detector after separation by reverse phase high pressure liquid chromatography (HPLC). Recoveries of zearalenone added to corn at levels from 10 to 200 ng/g averaged greater than 89%. In addition, a confirmation procedure is described which involves sequential HPLC analysis of the sample and a zearalenone standard, using 4 different excitation wavelengths and comparing fluorescence responses obtained. This method was successfully applied to the analysis of 11 samples of cornmeal; zearalenone was detected in 9 of the samples at levels from 11 to 69 ng/g.

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