Disposable Strip Biosensor for Visual Detection of Hg2+ Based on Hg2+-Triggered Toehold Binding and Exonuclease III-Assisted Signal Amplification

2014 ◽  
Vol 86 (6) ◽  
pp. 3108-3114 ◽  
Author(s):  
Junhua Chen ◽  
Shungui Zhou ◽  
Junlin Wen
Keyword(s):  
Signal Amplification ◽  
Visual Detection ◽  
Exonuclease Iii

scholarly journals Turn-On Fluorescence Aptasensor on Magnetic Nanobeads for Aflatoxin M1 Detection Based on an Exonuclease III-Assisted Signal Amplification Strategy

Nanomaterials ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 104
Author(s):  
Fuyuan Zhang ◽  
Linyang Liu ◽  
Shengnan Ni ◽  
Jiankang Deng ◽  
Guo-Jun Liu ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Aflatoxin M1 ◽  
Exonuclease Iii ◽  
Sensor Performance ◽  
Milk Samples ◽  
Increased Sensitivity ◽  
Exo Iii ◽  
Amplification Strategy ◽  
Optimized Conditions ◽  
Magnetic Nanobeads

In order to satisfy the need for sensitive detection of Aflatoxin M1 (AFM1), we constructed a simple and signal-on fluorescence aptasensor based on an autocatalytic Exonuclease III (Exo III)-assisted signal amplification strategy. In this sensor, the DNA hybridization on magnetic nanobeads could be triggered by the target AFM1, resulting in the release of a single-stranded DNA to induce an Exo III-assisted signal amplification, in which numerous G-quadruplex structures would be produced and then associated with the fluorescent dye to generate significantly amplified fluorescence signals resulting in the increased sensitivity. Under the optimized conditions, this aptasensor was able to detect AFM1 with a practical detection limit of 9.73 ng kg−1 in milk samples. Furthermore, the prepared sensor was successfully used for detection of AFM1 in the commercially available milk samples with the recovery percentages ranging from 80.13% to 108.67%. Also, the sensor performance was evaluated by the commercial immunoassay kit with satisfactory results.

Multi-signal amplification electrochemical DNA biosensor based on exonuclease III and tetraferrocene

2020 ◽  
Vol 8 (18) ◽  
pp. 4143-4150
Author(s):  
Zhaojiang Yin ◽  
Hanfeng Cui ◽  
Qingxia Shu ◽  
Chen Jin ◽  
Yan Lin ◽  
...  
Keyword(s):  
Benzoic Acid ◽  
Electrochemical Sensor ◽  
Signal Amplification ◽  
Dna Biosensor ◽  
Exonuclease Iii ◽  
Electrochemical Dna Biosensor ◽  
Exo Iii

A new and efficient signal marker, 3,5-bis(3,5-bisferrocenethoxybenzyloxy)benzoic acid, was firstly synthesized and used for labeling. Construct a homogeneous electrochemical sensor with Exo III assisting the target cycle.

Novel detection platform for insulin based on dual-cycle signal amplification by Exonuclease III

Talanta ◽  
2019 ◽  
Vol 199 ◽  
pp. 596-602
Author(s):  
Chen Liu ◽  
Jialun Han ◽  
Jingjing Zhang ◽  
Jie Du
Keyword(s):  
Signal Amplification ◽  
Exonuclease Iii ◽  
Dual Cycle

A sensitive immobilization-free electrochemical assay for T4PNK activity based on exonuclease III-assisted recycling

RSC Advances ◽  
2015 ◽  
Vol 5 (92) ◽  
pp. 75348-75353 ◽  
Author(s):  
Yonghong Wang ◽  
Yaohui Wu ◽  
Yuanqing Wang ◽  
Bo Zhou ◽  
Shun Wu
Keyword(s):  
Signal Amplification ◽  
Electrochemical Assay ◽  
Exonuclease Iii

This work develops a novel, immobilization-free, simple and sensitive electrochemical platform for the detection of T4PNK activity based on λ-exo and exonuclease III-assisted signal amplification.

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