A Highly Sensitive and Selective Electrochemical Biosensor for Direct Detection of MicroRNAs in Serum

2013 ◽  
Vol 85 (9) ◽  
pp. 4784-4789 ◽  
Author(s):  
Yuqian Ren ◽  
Huimin Deng ◽  
Wei Shen ◽  
Zhiqiang Gao
Keyword(s):  
Electrochemical Biosensor ◽  
Direct Detection ◽  
Highly Sensitive

A highly sensitive electrochemical microRNA-21 biosensor based on intercalating methylene blue signal amplification and a highly dispersed gold nanoparticles/graphene/polypyrrole composite

The Analyst ◽  
2021 ◽  
Vol 146 (8) ◽  
pp. 2679-2688
Author(s):  
Chammari Pothipor ◽  
Noppadol Aroonyadet ◽  
Suwussa Bamrungsap ◽  
Jaroon Jakmunee ◽  
Kontad Ounnunkad
Keyword(s):  
Methylene Blue ◽  
Gold Nanoparticles ◽  
Signal Amplification ◽  
Electrochemical Biosensor ◽  
Highly Sensitive ◽  
Highly Dispersed ◽  
Polypyrrole Composite ◽  
Amplification Strategy ◽  
Dispersed Gold ◽  
Potential Tool

An ultrasensitive electrochemical biosensor based on a gold nanoparticles/graphene/polypyrrole composite modified electrode and a signal amplification strategy employing methylene blue is developed as a potential tool for the detection of miRNA-21.

Electrochemical biosensor for detection of pathogenic bacteria based on dual signal amplification of Cu3(PO4)2-mediated click chemistry and DNAzyme

The Analyst ◽  
2021 ◽  
Author(s):  
Hongguo Wei ◽  
Jiayu Wan ◽  
Shengjun Bu ◽  
Wenguang Zhang ◽  
Ma Li ◽  
...  
Keyword(s):  
Click Chemistry ◽  
Magnetic Separation ◽  
Concanavalin A ◽  
Signal Amplification ◽  
Pathogenic Bacteria ◽  
Electrochemical Biosensor ◽  
Inorganic Hybrid ◽  
Highly Sensitive ◽  
Antibody Conjugates ◽  
Dual Signal Amplification

A novel electrochemical biosensor for detecting pathogenic bacteria was designed based on specific magnetic separation and highly sensitive click chemistry. Instead of enzyme-antibody conjugates, organic-inorganic hybrid nanoflowers (Concanavalin A (Con...

Development of Mlra Enzyme-Based Electrochemical Biosensor for the Direct Detection of MC-LR

2021 ◽  
Vol MA2021-02 (52) ◽  
pp. 1523-1523
Author(s):  
Dharanivasan Gunasekaran ◽  
Sefi Vernick
Keyword(s):  
Electrochemical Biosensor ◽  
Direct Detection

Intramolecular photoinitiator induced atom transfer radical polymerization for electrochemical DNA detection

The Analyst ◽  
2020 ◽  
Vol 145 (3) ◽  
pp. 858-864
Author(s):  
Ligang Ma ◽  
Qianrui Liu ◽  
Lihe Jian ◽  
Shan Ye ◽  
Xiaoke Zheng ◽  
...  
Keyword(s):  
Atom Transfer Radical Polymerization ◽  
Radical Polymerization ◽  
Electrochemical Biosensor ◽  
Dna Detection ◽  
Atom Transfer ◽  
Highly Sensitive ◽  
Electrochemical Dna Detection ◽  
First Time

A novel electrochemical biosensor was reported for the first time to achieve highly sensitive DNA detection based on photoinduced atom transfer radical polymerization (photoATRP).

Factor XIII activation peptide is released into plasma upon cleavage by thrombin and shows a different structure compared to its bound form

2007 ◽  
Vol 97 (06) ◽  
pp. 890-898 ◽  
Author(s):  
Verena Schroeder ◽  
Jean-Marc Vuissoz ◽  
Amedeo Caflisch ◽  
Hans Kohler
Keyword(s):  
Human Plasma ◽  
Factor Xiii ◽  
Direct Detection ◽  
Polyclonal Antibodies ◽  
Coagulation Factor ◽  
Highly Sensitive ◽  
Clinical Interest ◽  
Activation Peptide ◽  
Dynamics Simulations

SummaryThe first step of coagulation factor XIII (FXIII) activation involves cleavage of the FXIII activation peptide (FXIII-AP) by thrombin. However, it is not known whether the FXIII-AP is released into plasma upon cleavage or remains attached to activated FXIII. The aim of the present work was to study the structure of free FXIII-AP, develop an assay for FXIII-AP determination in human plasma, and to answer the question whether FXIII-AP is released into plasma. We used ab-initio modeling and molecular dynamics simulations to study the structure of free FXIII-AP. We raised monoclonal and polyclonal antibodies against FXIII-AP and developed a highly sensitive and specific ELISA method for direct detection of FXIII-AP in human plasma. Structural analysis showed a putative different conformation of the free FXIII-AP compared to FXIII-AP bound to the FXIII protein. We concluded that it might be feasible to develop specific antibodies against the free FXIII-AP. Using our new FXIII-AP ELISA, we found high levels of FXIII-AP in in-vitro activated plasma samples and serum. We showed for the first time that FXIIIAP is detached from activated FXIII and is released into plasma, where it can be directly measured. Our findings may be of major clinical interest in regard to a possible new marker in thrombotic disease.

scholarly journals Optimization of the Electrodeposition of Gold Nanoparticles for the Application of Highly Sensitive, Label-Free Biosensor

Biosensors ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 50 ◽  
Author(s):  
Hao-Chun Chiang ◽  
Yanyan Wang ◽  
Qi Zhang ◽  
Kalle Levon
Keyword(s):  
Gold Nanoparticles ◽  
Signal Amplification ◽  
Electrochemical Biosensor ◽  
Aqueous Electrolyte ◽  
Label Free ◽  
Reduction Step ◽  
Glucose Biosensors ◽  
Highly Sensitive ◽  
Amperometric Glucose Biosensors ◽  
Aunp Surface

A highly sensitive electrochemical biosensor with a signal amplification platform of electrodeposited gold nanoparticle (AuNP) has been developed and characterized. The sizes of the synthesized AuNP were found to be critical for the performance of biosensor in which the sizes were dependent on HAuCl4 and acid concentrations; as well as on scan cycles and scan rates in the gold electro-reduction step. Systematic investigations of the adsorption of proteins with different sizes from aqueous electrolyte solution onto the electrodeposited AuNP surface were performed with a potentiometric method and calibrated by design of experiment (DOE). The resulting amperometric glucose biosensors was demonstrated to have a low detection limit (> 50 μM) and a wide linear range after optimization with AuNP electrodeposition.

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