scholarly journals Label-Free Detection of Cardiac Troponin-I Using Carbon Nanofiber Based Nanoelectrode Arrays

2013 ◽  
Vol 85 (8) ◽  
pp. 3858-3863 ◽  
Author(s):  
Adaikkappan Periyakaruppan ◽  
Ram P. Gandhiraman ◽  
M. Meyyappan ◽  
Jessica E. Koehne
Keyword(s):  
Cardiac Troponin ◽  
Troponin I ◽  
Carbon Nanofiber ◽  
Cardiac Troponin I ◽  
Label Free ◽  
Label Free Detection ◽  
Nanoelectrode Arrays

scholarly journals Long-Range Surface Plasmon-Polariton Waveguide Biosensors for Human Cardiac Troponin I Detection

Sensors ◽  
2019 ◽  
Vol 19 (3) ◽  
pp. 631 ◽  
Author(s):  
Oleksiy Krupin ◽  
Pierre Berini
Keyword(s):  
Long Range ◽  
Surface Plasmon ◽  
Cardiac Troponin ◽  
Surface Plasmon Polariton ◽  
Troponin I ◽  
Biological Marker ◽  
Cardiac Troponin I ◽  
Label Free ◽  
Label Free Detection ◽  
Plasmon Polariton

Straight long-range surface plasmon-polariton (LRSPP) waveguides as biosensors for label-free detection are discussed. The sensors consist of 5-μm-wide 35-nm-thick gold stripes embedded in a low-index optical-grade fluoropolymer (CYTOPTM) with fluidic channels etched to the Au surface of the stripes. This work demonstrates the application of the LRSPP biosensors for the detection of human cardiac troponin I (cTnI) protein. cTnI is a biological marker for acute myocardial infarction (AMI), often referred to as a heart attack, which can be diagnosed by elevated levels of cTnI in patient blood. Direct and sandwich assays were developed and demonstrated over the concentration range from 1 to 1000 ng/mL, yielding detection limits of 430 pg/mL for the direct assay and 28 pg/mL for the sandwich assay (1 standard deviation), the latter being physiologically relevant to the early detection or onset of AMI. In addition, a novel approach for data analysis is proposed, where the analyte response is normalized to the response of the antibody layer.

scholarly journals Label-free detection of cardiac troponin I with a photonic crystal biosensor

2014 ◽  
Vol 58 ◽  
pp. 107-113 ◽  
Author(s):  
Bailin Zhang ◽  
Andres W. Morales ◽  
Ralph Peterson ◽  
Liang Tang ◽  
Jing Yong Ye
Keyword(s):  
Photonic Crystal ◽  
Cardiac Troponin ◽  
Troponin I ◽  
Cardiac Troponin I ◽  
Label Free ◽  
Label Free Detection

Normal-incidence type solution immersed silicon (SIS) biosensor for ultra-sensitive, label-free detection of cardiac troponin I

2020 ◽  
Vol 168 ◽  
pp. 112525
Author(s):  
Dong Hyung Kim ◽  
Won Chegal ◽  
Yong Jai Cho ◽  
Sang Won O ◽  
Long Van Le ◽  
...  
Keyword(s):  
Cardiac Troponin ◽  
Troponin I ◽  
Normal Incidence ◽  
Cardiac Troponin I ◽  
Label Free ◽  
Type Solution ◽  
Label Free Detection

A MXene-functionalized paper-based electrochemical immunosensor for label-free detection of cardiac troponin I

2021 ◽  
Vol 42 (9) ◽  
pp. 092601
Author(s):  
Li Wang ◽  
Yufeng Han ◽  
Hongchen Wang ◽  
Yaojie Han ◽  
Jinhua Liu ◽  
...  
Keyword(s):  
Cardiac Troponin ◽  
Troponin I ◽  
Electrochemical Immunosensor ◽  
Cardiac Troponin I ◽  
Label Free ◽  
Label Free Detection

scholarly journals Fluorescence Based on Surface Plasmon Coupled Emission for Ultrahigh Sensitivity Immunoassay of Cardiac Troponin I

Biomedicines ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 448
Author(s):  
Vien Thi Tran ◽  
Heongkyu Ju
Keyword(s):  
Surface Plasmon ◽  
Cardiac Troponin ◽  
Troponin I ◽  
Limit Of Detection ◽  
Delayed Diagnosis ◽  
Cardiac Troponin I ◽  
Quantitative Detection ◽  
Fluorescence Signal ◽  
Label Free ◽  
Ultrahigh Sensitivity

This work demonstrates the quantitative assay of cardiac Troponin I (cTnI), one of the key biomarkers for acute cardiovascular diseases (the leading cause of death worldwide) using the fluorescence-based sandwich immune reaction. Surface plasmon coupled emission (SPCE) produced by non-radiative coupling of dye molecules with surface plasmons being excitable via the reverse Kretschmann format is exploited for fluorescence-based sandwich immunoassay for quantitative detection of cTnI. The SPCE fluorescence chip utilizes the gold (2 nm)-silver (50 nm) bimetallic thin film, with which molecules of the dye Alexa 488 (conjugated with detection antibodies) make a near field coupling with the plasmonic film for SPCE. The experimental results find that the SPCE greatly improves the sensitivity via enhancing the fluorescence signal (up to 50-fold) while suppressing the photo-bleaching, permitting markedly enhanced signal-to-noise ratio. The limit of detection of 21.2 ag mL−1 (atto-gram mL−1) is obtained, the lowest ever reported to date amid those achieved by optical technologies such as luminescence and label-free optical sensing techniques. The features discovered such as ultrahigh sensitivity may prompt the presented technologies to be applied for early diagnosis of cTnI in blood, particularly for emergency medical centers overloaded with patients with acute myocardial infarction who would suffer from time-delayed diagnosis due to insufficient assay device sensitivity.

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