Graphene Signal Amplification for Sensitive and Real-Time Fluorescence Anisotropy Detection of Small Molecules

2013 ◽  
Vol 85 (3) ◽  
pp. 1424-1430 ◽  
Author(s):  
Jinhua Liu ◽  
Changyao Wang ◽  
Ying Jiang ◽  
Yaping Hu ◽  
Jishan Li ◽  
...  
Keyword(s):  
Small Molecules ◽  
Real Time ◽  
Fluorescence Anisotropy ◽  
Signal Amplification

Signal-amplification and real-time fluorescence anisotropy detection of apyrase by carbon nanoparticle

2014 ◽  
Vol 38 ◽  
pp. 206-211 ◽  
Author(s):  
Jinhua Liu ◽  
Jing Yu ◽  
Jianrong Chen ◽  
Ronghua Yang ◽  
Kaimin Shih
Keyword(s):  
Real Time ◽  
Fluorescence Anisotropy ◽  
Signal Amplification ◽  
Carbon Nanoparticle

scholarly journals Recent Advances in Application of Biosensors in Tissue Engineering

2014 ◽  
Vol 2014 ◽  
pp. 1-18 ◽  
Author(s):  
Anwarul Hasan ◽  
Md Nurunnabi ◽  
Mahboob Morshed ◽  
Arghya Paul ◽  
Alessandro Polini ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Small Molecules ◽  
Real Time ◽  
Inflammatory Cytokines ◽  
Serum Proteins ◽  
Molecular Size ◽  
Medical Diagnostics ◽  
Alpha Fetoprotein ◽  
Pharmaceutical Industries

Biosensors research is a fast growing field in which tens of thousands of papers have been published over the years, and the industry is now worth billions of dollars. The biosensor products have found their applications in numerous industries including food and beverages, agricultural, environmental, medical diagnostics, and pharmaceutical industries and many more. Even though numerous biosensors have been developed for detection of proteins, peptides, enzymes, and numerous other biomolecules for diverse applications, their applications in tissue engineering have remained limited. In recent years, there has been a growing interest in application of novel biosensors in cell culture and tissue engineering, for example, real-time detection of small molecules such as glucose, lactose, and H2O2as well as serum proteins of large molecular size, such as albumin and alpha-fetoprotein, and inflammatory cytokines, such as IFN-g and TNF-α. In this review, we provide an overview of the recent advancements in biosensors for tissue engineering applications.

scholarly journals Harnessing asymmetric N-heterocyclic carbene ligands to optimise SABRE hyperpolarisation

2018 ◽  
Vol 8 (19) ◽  
pp. 4925-4933 ◽  
Author(s):  
Chin Min Wong ◽  
Marianna Fekete ◽  
Rhianna Nelson-Forde ◽  
Mark R. D. Gatus ◽  
Peter J. Rayner ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Small Molecules ◽  
Signal Amplification ◽  
Exchange Process ◽  
Carbene Ligands

The catalytic signal amplification by reversible exchange process is used widely to improve the magnetic resonance detectability of small molecules by hyperpolarisation.

scholarly journals Probing the interaction of nanoparticles with small molecules in real time via quartz crystal microbalance monitoring

Nanoscale ◽  
2019 ◽  
Vol 11 (23) ◽  
pp. 11107-11113 ◽  
Author(s):  
Ye Yang ◽  
Guillaume Poss ◽  
Yini Weng ◽  
Runzhang Qi ◽  
Hanrui Zheng ◽  
...  
Keyword(s):  
Small Molecules ◽  
Real Time ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Binding Affinities ◽  
Target Analytes

We report on a novel screening route to obtain quantitative measures for nanoparticle binding affinities to target analytes.

Cyclic enzymatic repairing-mediated dual-signal amplification for real-time monitoring of thymine DNA glycosylase

2017 ◽  
Vol 53 (27) ◽  
pp. 3878-3881 ◽  
Author(s):  
Li-Juan Wang ◽  
Zi-Yue Wang ◽  
Qianyi Zhang ◽  
Bo Tang ◽  
Chun-Yang Zhang
Keyword(s):  
Real Time ◽  
Signal Amplification ◽  
Fluorescence Method ◽  
Dna Glycosylase ◽  
Real Time Monitoring ◽  
Thymine Dna Glycosylase ◽  
Dual Signal Amplification

We develop a new fluorescence method for real-time monitoring of thymine DNA glycosylase activity through cyclic enzymatic repairing-mediated dual-signal amplification.

Target-directed enzyme-free dual-amplification DNA circuit for rapid signal amplification

2020 ◽  
Vol 8 (47) ◽  
pp. 10770-10775
Author(s):  
Chao Xing ◽  
Ziyi Chen ◽  
Cheng Zhang ◽  
Jun Wang ◽  
Chunhua Lu
Keyword(s):  
Nucleic Acids ◽  
Small Molecules ◽  
Signal Amplification ◽  
Fluorescence Analysis ◽  
Single Step ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Dual Amplification

An enzyme-free, single-step and rapid signal amplification DNA circuit was developed by integrating target-directed entropy-driven catalysis and hybridization chain reaction for fluorescence analysis of nucleic acids and small molecules.

scholarly journals Real-Time In Vitro Fluorescence Anisotropy of the Cyanobacterial Circadian Clock

2019 ◽  
Vol 2 (2) ◽  
pp. 42 ◽  
Author(s):  
Joel Heisler ◽  
Archana Chavan ◽  
Yong-Gang Chang ◽  
Andy LiWang
Keyword(s):  
Circadian Clock ◽  
Real Time ◽  
Fluorescence Anisotropy ◽  
Polyacrylamide Gel Electrophoresis ◽  
Live Cells ◽  
Ex Post ◽  
Clock Proteins ◽  
Ex Post Facto ◽  
Clock Reaction

Uniquely, the circadian clock of cyanobacteria can be reconstructed outside the complex milieu of live cells, greatly simplifying the investigation of a functioning biological chronometer. The core oscillator component is composed of only three proteins, KaiA, KaiB, and KaiC, and together with ATP they undergo waves of assembly and disassembly that drive phosphorylation rhythms in KaiC. Typically, the time points of these reactions are analyzed ex post facto by denaturing polyacrylamide gel electrophoresis, because this technique resolves the different states of phosphorylation of KaiC. Here, we describe a more sensitive method that allows real-time monitoring of the clock reaction. By labeling one of the clock proteins with a fluorophore, in this case KaiB, the in vitro clock reaction can be monitored by fluorescence anisotropy on the minutes time scale for weeks.

Sign in / Sign up

Export Citation Format

Share Document