Strategy for Minimizing Between-Study Variation of Large-Scale Phenotypic Experiments Using Multivariate Analysis

2012 ◽  
Vol 84 (20) ◽  
pp. 8675-8681 ◽  
Author(s):  
Rui C. Pinto ◽  
Lorenz Gerber ◽  
Mattias Eliasson ◽  
Björn Sundberg ◽  
Johan Trygg
Keyword(s):  
Multivariate Analysis ◽  
Large Scale

Algorithm of combining chromatography mass spectrometry-untargeted profiling and multivariate analysis for identification of marker-substances in samples of complex composition

2020 ◽  
Vol 86 (7) ◽  
pp. 12-19
Author(s):  
I. V. Plyushchenko ◽  
D. G. Shakhmatov ◽  
I. A. Rodin
Keyword(s):  
Mass Spectrometry ◽  
Multivariate Analysis ◽  
Large Scale ◽  
Complex Composition ◽  
Unified Protocol ◽  
Chromatography Mass Spectrometry ◽  
Marker Substances ◽  
Selection Testing ◽  
Untargeted Profiling

A viral development of statistical data processing, computing capabilities, chromatography-mass spectrometry, and omics technologies (technologies based on the achievements of genomics, transcriptomics, proteomics, metabolomics) in recent decades has not led to formation of a unified protocol for untargeted profiling. Systematic errors reduce the reproducibility and reliability of the obtained results, and at the same time hinder consolidation and analysis of data gained in large-scale multi-day experiments. We propose an algorithm for conducting omics profiling to identify potential markers in the samples of complex composition and present the case study of urine samples obtained from different clinical groups of patients. Profiling was carried out by the method of liquid chromatography mass spectrometry. The markers were selected using methods of multivariate analysis including machine learning and feature selection. Testing of the approach was performed using an independent dataset by clustering and projection on principal components.

scholarly journals Identification of factors and groups at risk of infection with Schistosoma mansoni: a strategy for the implementation of control measures?

1994 ◽  
Vol 36 (3) ◽  
pp. 245-253 ◽  
Author(s):  
Pedro Coura-Filho ◽  
Roberto Sena Rocha ◽  
Marcio Willian Farah ◽  
Grace Carolina da Silva ◽  
Naftale Katz
Keyword(s):  
Risk Factors ◽  
Multivariate Analysis ◽  
Urban Area ◽  
Rural Area ◽  
Large Scale ◽  
Geometric Mean ◽  
Control Program ◽  
Control Measures ◽  
Agricultural Activity ◽  
Domestic Water

A fourteen year schistosomiasis control program in Peri-Peri (Capim Branco, MG) reduced prevalence from 43.5 to 4.4%; incidence from 19.0 to 2.9%, the geometric mean of the number of eggs from 281 to 87 and the level of the hepatoesplenic form cases from 5.9 to 0.0%. In 1991, three years after the interruption of the program, the prevalence had risen to 19.6%. The district consists of Barbosa (a rural area) and Peri-Peri itself (an urban area). In 1991, the prevalence in the two areas was 28.4% and 16.0% respectively. A multivariate analysis of risk factors for schistosomiasis indicated the domestic agricultural activity with population attributive risk (PAR) of 29.82%, the distance (< 10 m) from home to water source (PAR = 25.93%) and weekly fishing (PAR = 17.21%) as being responsible for infections in the rural area. The recommended control measures for this area are non-manual irrigation and removal of homes to more than ten meters from irrigation ditches. In the urban area, it was observed that swimming at weekly intervals (PAR = 20.71%), daily domestic agricultural activity (PAR = 4.07%) and the absence of drinking water in the home (PAR=4.29%) were responsible for infections. Thus, in the urban area the recommended control measures are the substitution of manual irrigation with an irrigation method that avoids contact with water, the creation of leisure options of the population and the provision of a domestic water supply. The authors call attention to the need for the efficacy of multivariate analysis of risk factors to be evaluated for schistosomiasis prior to its large scale use as a indicator of the control measures to be implemented.

Sex differences in atrial fibrillation ablation outcomes: insights from a large-scale multicentre registry

EP Europace ◽  
2020 ◽  
Vol 22 (9) ◽  
pp. 1345-1357 ◽  
Author(s):  
Nobuaki Tanaka ◽  
Koichi Inoue ◽  
Atsushi Kobori ◽  
Kazuaki Kaitani ◽  
Takeshi Morimoto ◽  
...  
Keyword(s):  
Atrial Fibrillation ◽  
Multivariate Analysis ◽  
Sex Differences ◽  
Clinical Outcomes ◽  
Large Scale ◽  
De Novo ◽  
Long Term Follow Up ◽  
Af Recurrence

Abstract Aim The impact of sex differences on the clinical outcomes of radiofrequency catheter ablation (RFCA) of atrial fibrillation (AF) is controversial. We investigated the sex differences regarding the efficacy and clinical outcomes of RFCA of AF. Methods and results We conducted a large-scale, prospective, multicentre, observational study (Kansai Plus Atrial Fibrillation Registry). We enrolled 5010 consecutive patients who underwent an initial RFCA of AF at 26 centres (64 ± 10 years; non-paroxysmal AF, 35.7%). The median follow-up duration was 2.9 years. Female patients (n = 1369, 27.3%) were older (female vs. male, 68 ± 9 vs. 63 ± 11 years, P &lt; 0.0001) with a lower prevalence of non-paroxysmal AF (27.1% vs. 38.9%, P &lt; 0.0001). Fewer females experienced time-dependent pulmonary vein (PV) reconnections and more females received a non-PV foci ablation than males in the index RFCA. The 3-year cumulative incidence of AF recurrences in the multivariate analysis after single procedures was significantly higher in females than males (43.3% vs. 39.0%, log rank P = 0.0046). Females remained an independent predictor of AF recurrence (hazard ratio 1.24; 95% confidence interval 1.12–1.38, P &lt; 0.0001). The AF recurrence rates after multiple procedures were also higher in females, but fewer females experienced PV reconnections during second sessions. More females experienced de novo pacemaker implantations during the long-term follow-up. Females were associated with a higher risk of heart failure hospitalizations and major bleeding after RFCA in the multivariate analysis. Conclusions Females experienced more frequent AF recurrences probably due to non-PV arrhythmogenicity and de novo pacemaker implantations than males during the long-term follow-up after RFCA of AF.

Large-scale multivariate analysis reveals sexual dimorphism and geographic differences in the Gray's beaked whale

2014 ◽  
Vol 294 (1) ◽  
pp. 13-21 ◽  
Author(s):  
K. F. Thompson ◽  
K. Ruggiero ◽  
C. D. Millar ◽  
R. Constantine ◽  
A. L. van Helden
Keyword(s):  
Multivariate Analysis ◽  
Sexual Dimorphism ◽  
Large Scale ◽  
Beaked Whale ◽  
Geographic Differences

Similar Background of Graft-Versus-Host Disease (GVHD) Following Allogeneic Blood and Marrow Transplantation (Tx) in Korean and Japanese Populations Allows a Uniform Foundation To Perform Large-Scale Clinical Studies in the Region.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 5007-5007
Author(s):  
Sung-Won Kim ◽  
Jong-Wook Lee ◽  
Hyeoung-Joon Kim ◽  
Takahiro Fukuda ◽  
Yoshinobu Kanda ◽  
...  
Keyword(s):  
Multivariate Analysis ◽  
Risk Factor ◽  
High Risk ◽  
Large Scale ◽  
Conditioning Regimen ◽  
Disease Status ◽  
Underlying Disease ◽  
High Risk Disease ◽  
Grade Ii

Abstract Background: Although it has been reported that the incidence of acute GVHD (aGVHD) and early non-relapse mortality (NRM) after HLA-identical sibling BMT are different between Japanese and other ethnicities, including white American, African American and Irish populations (Oh, Blood.2005), there has been no previous comparison among Asian countries. Patients and Methods: We retrospectively surveyed the data of 1933 patients (pts) (996 Korean and 937 Japanese) who underwent allo-Tx for leukemia or MDS between Jan, 2000 and Dec, 2005. The median age of the pts was 37 yrs (range, 16–70; 34 yrs vs 43 yrs, respectively) and the median follow-up of surviving pts was 1046 days (11–2435). Unexpectedly, the pts’ backgrounds, including age, underlying disease, disease status at Tx, stem cell source, donor type, No. of HLA-mismatched (MM) antigen/allele, conditioning regimen, in vivo T-cell depletion with ATG or Campath-1H and GVHD prophylaxis were significantly different between the two groups. Results: (Table) In a multivariate analysis, Tx from a serologically HLA-1-antigen MM donor, Tx from an unrelated donor (URD), pts with high-risk disease at Tx, no in vivo T-cell depletion and a conditioning regimen that included TBI ≥ 10 Gy were associated with an increased risk of grade II–IV aGVHD. Overall, race was not a risk factor for grade II–IV (P=0.19) or III–IV (P=0.98) aGVHD. The incidence of chronic GVHD (cGVHD) in Japanese was significantly lower than that in Korean (P=0.016). In a multivariate analysis, Tx from a genetically HLA-allele MM donor, Tx from an URD, pts with performance status 2–4 at Tx, pts with high-risk disease at Tx, pts with ALL, female to male Tx and pts’ age ≥ 40 yrs were associated with poor OS. Race was not a risk factor for OS. The incidence of relapse adjusted for the underlying disease status was associated with grade II–IV aGVHD (RR 0.75, 95% CI 0.61–0.94, P=0.010), but not cGVHD (P=0.63). Conclusions: Despite their different medical backgrounds, our data confirmed that the biology of GVHD is very similar between these two Asian populations, and this provides a uniform foundation for large-scale clinical studies in the region. Korean (n=996) Japanese (n=937) Grade II–IV/III–IV aGVHD 37%/15% 44%/19% Median onset of aGVHD day 28 day 21 cGVHD 60% 47% 3-yr OS in standard/high risk 70%/42% 65%/41% NRM 25% 30% Relapse 25% 24%

scholarly journals Association between schizophrenia and an autoimmune bullous skin disease-pemphigus: a population-based large-scale study

2017 ◽  
Vol 28 (2) ◽  
pp. 191-198 ◽  
Author(s):  
K. Kridin ◽  
S. Zelber-Sagi ◽  
D. Comaneshter ◽  
A. D. Cohen
Keyword(s):  
Multivariate Analysis ◽  
Large Scale ◽  
Matched Control ◽  
Univariate Analysis ◽  
Real Life ◽  
Population Based ◽  
Cross Sectional Study ◽  
Control Group ◽  
Cross Sectional ◽  
Wide Range

Aims.Immunological hypotheses have become increasingly prominent suggesting that autoimmunity may be involved in the pathogenesis of schizophrenia. Schizophrenia was found to be associated with a wide range of autoimmune diseases. However, the association between pemphigus and schizophrenia has not been established yet. We aimed to estimate the association between pemphigus and schizophrenia using a large-scale real-life computerised database.Methods.This study was conducted as a cross-sectional study utilising the database of Clalit Health Services. The proportion of schizophrenia was compared between patients diagnosed with pemphigus and age-, gender- and ethnicity-matched control subjects. Univariate analysis was performed usingχ2and Student'st-test and a multivariate analysis was performed using a logistic regression model.Results.A total of 1985 pemphigus patients and 9874 controls were included in the study. The prevalence of schizophrenia was greater in patients with pemphigus as compared to the control group (2.0%v. 1.3%, respectively;p= 0.019). In a multivariate analysis, pemphigus was significantly associated with schizophrenia (OR, 1.5; 95% CI, 1.1–2.2). The association was more prominent among females, patients older than 60 years, and Jews.Conclusions.Pemphigus is significantly associated with schizophrenia. Physicians treating patients with pemphigus should be aware of this possible association. Patients with pemphigus should be carefully assessed for comorbid schizophrenia and be treated appropriately.

scholarly journals Efficient degradation of solid yeast biomass from ethanol industry by Fenton and UV-Fenton processes applying multivariate analysis

2017 ◽  
Vol 12 (6) ◽  
pp. 946
Author(s):  
Geórgia Labuto ◽  
Lilia Ribeiro da Silva ◽  
Heron Dominguez Torres da Silva ◽  
Elma Neide Vasconcelos Martins Carrilho ◽  
Norberto Sanches Goncalves ◽  
...  
Keyword(s):  
Multivariate Analysis ◽  
Experimental Design ◽  
Organic Contaminants ◽  
Large Scale ◽  
Oxidative Process ◽  
Alcohol Industry ◽  
Environmental Friendly ◽  
Yeast Biomass ◽  
The Media ◽  
Advanced Oxidative Process

Organic agro-industrial residues have been successfully used as biosorbents and promoting new uses from agricultural wastes benefits the economy. However, the allocation of a solid waste biosorbent after the sorption of contaminants has limited their effective application on a large scale as an alternative treatment of water and wastewaters. One solution could be degradation to convert the biosorbent material and adsorbed organic contaminants into environmental friendly compounds suitable for discharge. This study used an experimental design to evaluate the Fenton degradation of yeast biomass (YB) from the alcohol industry as a potential biosorbent. The efficiency of degradation was monitored according to the degraded mass (DM) and total organic carbon (TOC) remaining in the solution. The ANOVA showed an error of 9.7% for the effects and the media of interaction for the employed model for DM. Conducting the experiments with the best-predicted conditions (60 min, 25 g of YB, pH 3, 8,000 mg L-1 H2O2 and 40 mg L-1 Fe2+) with 30 W UV irradiation resulted in a YB reduction of 72 ± 2% with a TOC of 30 ± 2%. This suggests that an advanced oxidative process is an alternative for degradation of a biosorbent after sorption.

scholarly journals Can patients with mild post-operative hyponatraemia following elective arthroplasty be discharged safely? A large-scale service evaluation suggests they can

2021 ◽  
pp. 000456322110515
Author(s):  
Jonathan Macdonald ◽  
Emma Cunningham ◽  
Nicola Gallagher ◽  
Paul Hamilton ◽  
Roslyn Cassidy ◽  
...  
Keyword(s):  
Logistic Regression ◽  
Multivariate Analysis ◽  
Regression Analysis ◽  
Large Scale ◽  
Best Practice ◽  
Service Evaluation ◽  
Extensive Investigation ◽  
Electronic Database ◽  
Total Knee ◽  
Time Periods

Background Post-operative hyponatraemia is common following arthroplasty. Clinical hyponatraemia guidelines lack detail on when treatment is necessary, and there is a paucity of literature to guide best practice. Methods Data were collected within retrospective service evaluations over two time periods in a single high throughput joint unit. The hospital’s electronic database identified 1000 patients who were admitted electively between February 2012 and June 2013 and again between November 2018 and April 2019 for primary total hip, total knee or uni-compartmental knee arthroplasty. Hyponatraemia and non-hyponatraemia groups were compared. Logistic regression analysis was used to identify independent predictors of post-operative hyponatraemia, length of stay (LOS), re-attendance or re-admission to hospital. Results Between 2012–2013 and 2018–2019, 32.1% and 25.7% of patients, respectively, developed post-operative hyponatraemia (serum sodium (s[Na]) ≤135 mmol/L). Those with post-operative hyponatraemia were significantly older, weighed less, were more comorbid and had lower pre-operative sodium. Multivariate analysis showed that increased age, knee surgery and lower pre-operative s[Na] independently predicted post-operative hyponatraemia. Post-operative hyponatraemia did not independently predict LOS, re-attendance or re-admission to hospital, within 90 days, in either cohort. Conclusion Post-operative hyponatraemia is common and may be a marker of pre-operative vulnerability. In these cohorts, it was not independently associated with LOS, re-attendance or re-admission to hospital. We suggest that otherwise well patients with mild hyponatraemia can be safely discharged earlier than is often the case and may not require extensive investigation. Further examination and research is required to develop a pre-operative approach to predict which patients will develop significant post-operative hyponatraemia.

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