Label-free and reagentless electrochemical detection of PCR fragments using self-assembled quinone derivative monolayer: Application to Mycobacterium tuberculosis

2012 ◽  
Vol 32 (1) ◽  
pp. 163-168 ◽  
Author(s):  
Q.D. Zhang ◽  
G. March ◽  
V. Noel ◽  
B. Piro ◽  
S. Reisberg ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Electrochemical Detection ◽  
Label Free ◽  
Self Assembled ◽  
Quinone Derivative

Label-free electrochemical detection of HepG2 tumor cells with a self-assembled DNA nanostructure-based aptasensor

2018 ◽  
Vol 268 ◽  
pp. 359-367 ◽  
Author(s):  
Duanping Sun ◽  
Jing Lu ◽  
Dabin Chen ◽  
Yunfei Jiang ◽  
Zhiru Wang ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Electrochemical Detection ◽  
Label Free ◽  
Dna Nanostructure ◽  
Self Assembled

Label-free and dual-amplified electrochemical detection of Hg2+ based on self-assembled DNA nanostructures and target-triggered exonuclease cleavage activity

2016 ◽  
Vol 40 (8) ◽  
pp. 6686-6691 ◽  
Author(s):  
Ting Bao ◽  
Wei Wen ◽  
Lei Shu ◽  
Xiuhua Zhang ◽  
Shengfu Wang
Keyword(s):  
Electrochemical Detection ◽  
Signal Amplification ◽  
Electrochemical Biosensor ◽  
Dna Nanostructures ◽  
Label Free ◽  
Target Recycling ◽  
Cleavage Activity ◽  
Self Assembled ◽  
Exo Iii

An electrochemical biosensor for Hg2+ detection via HCR and Hg2+-triggered Exo III-assisted target recycling for signal amplification.

scholarly journals Label-Free Comparative Proteomics of Differentially Expressed Mycobacterium tuberculosis Protein in Rifampicin-Related Drug-Resistant Strains

Pathogens ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 607
Author(s):  
Nadeem Ullah ◽  
Ling Hao ◽  
Jo-Lewis Banga Ndzouboukou ◽  
Shiyun Chen ◽  
Yaqi Wu ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Drug Targets ◽  
Control Strategies ◽  
Multidrug Resistant ◽  
Differentially Expressed ◽  
Effective Control ◽  
Drug Resistant ◽  
Differentially Expressed Proteins ◽  
Label Free ◽  
Candidate Target

Rifampicin (RIF) is one of the most important first-line anti-tuberculosis (TB) drugs, and more than 90% of RIF-resistant (RR) Mycobacterium tuberculosis clinical isolates belong to multidrug-resistant (MDR) and extensively drug-resistant (XDR) TB. In order to identify specific candidate target proteins as diagnostic markers or drug targets, differential protein expression between drug-sensitive (DS) and drug-resistant (DR) strains remains to be investigated. In the present study, a label-free, quantitative proteomics technique was performed to compare the proteome of DS, RR, MDR, and XDR clinical strains. We found iniC, Rv2141c, folB, and Rv2561 were up-regulated in both RR and MDR strains, while fadE9, espB, espL, esxK, and Rv3175 were down-regulated in the three DR strains when compared to the DS strain. In addition, lprF, mce2R, mce2B, and Rv2627c were specifically expressed in the three DR strains, and 41 proteins were not detected in the DS strain. Functional category showed that these differentially expressed proteins were mainly involved in the cell wall and cell processes. When compared to the RR strain, Rv2272, smtB, lpqB, icd1, and folK were up-regulated, while esxK, PPE19, Rv1534, rpmI, ureA, tpx, mpt64, frr, Rv3678c, esxB, esxA, and espL were down-regulated in both MDR and XDR strains. Additionally, nrp, PPE3, mntH, Rv1188, Rv1473, nadB, PPE36, and sseA were specifically expressed in both MDR and XDR strains, whereas 292 proteins were not identified when compared to the RR strain. When compared between MDR and XDR strains, 52 proteins were up-regulated, while 45 proteins were down-regulated in the XDR strain. 316 proteins were especially expressed in the XDR strain, while 92 proteins were especially detected in the MDR strain. Protein interaction networks further revealed the mechanism of their involvement in virulence and drug resistance. Therefore, these differentially expressed proteins are of great significance for exploring effective control strategies of DR-TB.

Construction of electrochemical immunosensor based on redox hydrogel for ultrasensitive detection of carcinoembryonic antigen

2021 ◽  
Author(s):  
Xiangrong Huang ◽  
Na Wu ◽  
Wenxiu Liu ◽  
Yazhuo Shang ◽  
Honglai Liu ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Carcinoembryonic Antigen ◽  
Electrochemical Detection ◽  
Electrochemical Immunosensor ◽  
Ultrasensitive Detection ◽  
Label Free

In this work, a novel redox hydrogel was proposed for ultrasensitive label-free electrochemical detection of carcinoembryonic antigen (CEA). The redox hydrogel composed by cellulose nanocrystalline (CNC), methylene blue (MB), multi-walled...

scholarly journals Microfluidic Impedance Biosensor Chips Using Sensing Layers Based on DNA-Based Self-Assembled Monolayers for Label-Free Detection of Proteins

Biosensors ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 80
Author(s):  
Khaled Alsabbagh ◽  
Tim Hornung ◽  
Achim Voigt ◽  
Sahba Sadir ◽  
Taleieh Rajabi ◽  
...  
Keyword(s):  
Troponin I ◽  
Electrochemical Impedance ◽  
Specific Binding ◽  
Self Assembled Monolayers ◽  
Significant Shift ◽  
Label Free ◽  
Label Free Detection ◽  
Assembled Monolayers ◽  
Self Assembled ◽  
Biosensor Chip

A microfluidic chip for electrochemical impedance spectroscopy (EIS) is presented as bio-sensor for label-free detection of proteins by using the example of cardiac troponin I. Troponin I is one of the most specific diagnostic serum biomarkers for myocardial infarction. The microfluidic impedance biosensor chip presented here consists of a microscope glass slide serving as base plate, sputtered electrodes, and a polydimethylsiloxane (PDMS) microchannel. Electrode functionalization protocols were developed considering a possible charge transfer through the sensing layer, in addition to analyte-specific binding by corresponding antibodies and reduction of nonspecific protein adsorption to prevent false-positive signals. Reagents tested for self-assembled monolayers (SAMs) on gold electrodes included thiolated hydrocarbons and thiolated oligonucleotides, where SAMs based on the latter showed a better performance. The corresponding antibody was covalently coupled on the SAM using carbodiimide chemistry. Sampling and measurement took only a few minutes. Application of a human serum albumin (HSA) sample, 1000 ng/mL, led to negligible impedance changes, while application of a troponin I sample, 1 ng/mL, led to a significant shift in the Nyquist plot. The results are promising regarding specific detection of clinically relevant concentrations of biomarkers, such as cardiac markers, with the newly developed microfluidic impedance biosensor chip.

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