Highly Efficient Assay of Circulating Tumor Cells by Selective Sedimentation with a Density Gradient Medium and Microfiltration from Whole Blood

2012 ◽  
Vol 84 (17) ◽  
pp. 7400-7407 ◽  
Author(s):  
Jong-Myeon Park ◽  
June-Young Lee ◽  
Jeong-Gun Lee ◽  
Hyoyoung Jeong ◽  
Jin-Mi Oh ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Density Gradient ◽  
Circulating Tumor Cells ◽  
Whole Blood ◽  
Highly Efficient ◽  
Density Gradient Medium ◽  
Gradient Medium

scholarly journals Aptamer-Conjugated Graphene Oxide Membranes for Highly Efficient Capture and Accurate Identification of Multiple Types of Circulating Tumor Cells

2015 ◽  
Vol 26 (2) ◽  
pp. 235-242 ◽  
Author(s):  
Bhanu Priya Viraka Nellore ◽  
Rajashekhar Kanchanapally ◽  
Avijit Pramanik ◽  
Sudarson Sekhar Sinha ◽  
Suhash Reddy Chavva ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Accurate Identification ◽  
Highly Efficient ◽  
Oxide Membranes ◽  
Graphene Oxide Membranes

A Microfluidic Platform for On-Chip Analysis of Circulating Tumor Cells

2021 ◽  
Author(s):  
Jeff Darabi ◽  
Joseph Schober
Keyword(s):  
Cancer Cells ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Whole Blood ◽  
Peripheral Blood ◽  
Automated Image Analysis ◽  
Rare Cancer ◽  
On Chip ◽  
Chip Analysis ◽  
Selection Of

Abstract Studies have shown that primary tumor sites begin shedding cancerous cells into peripheral blood at early stages of cancer, and the presence and frequency of circulating tumor cells (CTCs) in blood is directly proportional to disease progression. The challenge is that the concentration of the CTCs in peripheral blood may be extremely low. In the past few years, several microfluidic-based concepts have been investigated to isolate CTCs from whole blood. However, these devices are generally hampered by complex fabrication processes and very low volumetric throughputs, which may not be practical for rapid clinical applications. This paper presents a high-performance yet simple magnetophoretic microfluidic chip for the enrichment and on-chip analysis of rare CTCs from blood. Microscopic and flow cytometric assays developed for selection of cancer cell lines, selection of monoclonal antibodies, and optimization of bead coupling are discussed. Additionally, on-chip characterization of rare cancer cells using high resolution immunofluorescence microscopy and modeling results for prediction of CTC capture length are presented. The device has the ability to interface directly with on-chip pre and post processing modules such as mixing, incubation, and automated image analysis systems. These features will enable us to isolate rare cancer cells from whole blood and detect them on the chip with subcellular resolution.

Ex VivoDetection of Circulating Tumor Cells from Whole Blood by Direct Nanoparticle Visualization

ACS Nano ◽  
2018 ◽  
Vol 12 (2) ◽  
pp. 1902-1909 ◽  
Author(s):  
Regivaldo G. Sobral-Filho ◽  
Lindsay DeVorkin ◽  
Sarah Macpherson ◽  
Andrew Jirasek ◽  
Julian J. Lum ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Whole Blood

Integration of aligned polymer nanofibers within a microfluidic chip for efficient capture and rapid release of circulating tumor cells

2018 ◽  
Vol 2 (5) ◽  
pp. 891-900 ◽  
Author(s):  
Yunchao Xiao ◽  
Mengyuan Wang ◽  
Lizhou Lin ◽  
Lianfang Du ◽  
Mingwu Shen ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Microfluidic Chip ◽  
Polymer Nanofibers ◽  
Highly Efficient ◽  
Rapid Release ◽  
Aligned Nanofibers

Zwitterion-functionalized aligned nanofibers integrated with a microfluidic chip can be used for highly efficient capture and rapid release of CTCs.

scholarly journals Hybrid negative enrichment of circulating tumor cells from whole blood in a 3D-printed monolithic device

Lab on a Chip ◽  
2019 ◽  
Vol 19 (20) ◽  
pp. 3427-3437 ◽  
Author(s):  
Chia-Heng Chu ◽  
Ruxiu Liu ◽  
Tevhide Ozkaya-Ahmadov ◽  
Mert Boya ◽  
Brandi E. Swain ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Microfluidic Device ◽  
Whole Blood ◽  
3D Printed

A monolithic 3D-printed microfluidic device integrated with stacked layers of functionalized leukodepletion channels and microfiltration for the negative enrichment of circulating tumor cells directly from clinically relevant volumes of whole blood.

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