Carbonyl-Reactive Tandem Mass Tags for the Proteome-Wide Quantification of N-Linked Glycans

2012 ◽  
Vol 84 (8) ◽  
pp. 3716-3724 ◽  
Author(s):  
Hannes Hahne ◽  
Patrick Neubert ◽  
Karsten Kuhn ◽  
Chris Etienne ◽  
Ryan Bomgarden ◽  
...  
Keyword(s):  
Tandem Mass ◽  
Tandem Mass Tags

Exploration of the effect of salvianolate on myocardial infarction in rats based on tandem mass tags

2020 ◽  
Vol 889 ◽  
pp. 173610
Author(s):  
Cheng Chen ◽  
Pengli Zhu ◽  
Huizhen Yu ◽  
Bin Huang ◽  
Mengxuan Gui ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Tandem Mass ◽  
Tandem Mass Tags

scholarly journals Quantitative Proteomics Analysis of Altered Protein Expression in the Placental Villous Tissue of Early Pregnancy Loss Using Isobaric Tandem Mass Tags

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Xiaobei Ni ◽  
Xin Li ◽  
Yueshuai Guo ◽  
Tao Zhou ◽  
Xuejiang Guo ◽  
...  
Keyword(s):  
Protein Expression ◽  
Pregnant Women ◽  
Early Pregnancy ◽  
Proteomic Analysis ◽  
Pregnancy Loss ◽  
Tandem Mass ◽  
Pathophysiological Mechanism ◽  
Early Pregnancy Loss ◽  
Expression Levels ◽  
Tandem Mass Tags

Many pregnant women suffer miscarriages during early gestation, but the description of these early pregnancy losses (EPL) can be somewhat confusing because of the complexities of early development. Thus, the identification of proteins with different expression profiles related to early pregnancy loss is essential for understanding the comprehensive pathophysiological mechanism. In this study, we report a gel-free tandem mass tags- (TMT-) labeling based proteomic analysis of five placental villous tissues from patients with early pregnancy loss and five from normal pregnant women. The application of this method resulted in the identification of 3423 proteins and 19647 peptides among the patient group and the matched normal control group. Qualitative and quantitative proteomic analysis revealed 51 proteins to be differentially abundant between the two groups (≥1.2-fold, Student'st-test,P<0.05). To obtain an overview of the biological functions of the proteins whose expression levels altered significantly in EPL group, gene ontology analysis was performed. We also investigated the twelve proteins with a difference over 1.5-fold using pathways analysis. Our results demonstrate that the gel-free TMT-based proteomic approach allows the quantification of differences in protein expression levels, which is useful for obtaining molecular insights into early pregnancy loss.

scholarly journals Accurate and Sensitive Quantitation of the Dynamic Heat Shock Proteome Using Tandem Mass Tags

2020 ◽  
Vol 19 (3) ◽  
pp. 1183-1195 ◽  
Author(s):  
Aaron J. Storey ◽  
Rebecca E. Hardman ◽  
Stephanie D. Byrum ◽  
Samuel G. Mackintosh ◽  
Rick D. Edmondson ◽  
...  
Keyword(s):  
Heat Shock ◽  
Tandem Mass ◽  
Tandem Mass Tags

scholarly journals Evosep One Enables Robust Deep Proteome Coverage Using Tandem Mass Tags while Significantly Reducing Instrument Time

2019 ◽  
Vol 18 (5) ◽  
pp. 2346-2353 ◽  
Author(s):  
Jonathan R. Krieger ◽  
Leanne E. Wybenga-Groot ◽  
Jiefei Tong ◽  
Nicolai Bache ◽  
Ming S. Tsao ◽  
...  
Keyword(s):  
Tandem Mass ◽  
Proteome Coverage ◽  
Tandem Mass Tags

scholarly journals Tandem Mass Tags:  A Novel Quantification Strategy for Comparative Analysis of Complex Protein Mixtures by MS/MS

2006 ◽  
Vol 78 (12) ◽  
pp. 4235-4235 ◽  
Author(s):  
Andrew Thompson ◽  
Juergen Schaefer ◽  
Karsten Kuhn ◽  
Stefan Kienle ◽  
Josef Schwarz ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Tandem Mass ◽  
Complex Protein ◽  
Tandem Mass Tags

scholarly journals Phenotypic Analysis Combined with Tandem Mass Tags (TMT) Labeling Reveal the Heterogeneity of Strawberry Stolon Buds

2019 ◽  
Author(s):  
Ling Guan ◽  
Mizhen Zhao ◽  
Yaming Qian ◽  
Hongmei Yu ◽  
Jin Xia ◽  
...  
Keyword(s):  
Gene Networks ◽  
Cinnamyl Alcohol Dehydrogenase ◽  
Axillary Bud ◽  
Transcriptional Level ◽  
Tandem Mass ◽  
Phenotypic Analysis ◽  
Minichromosome Maintenance ◽  
Shoot Initiation ◽  
Phenylpropanoid Biosynthesis ◽  
Tandem Mass Tags

Abstract Background: Ramet propagation in strawberry (Fragaria × ananassa) is the most effective way in production. However, the lack of systematically phenotypic observations and high-throughput methods limits our ability to analyze the key factors regulating the heterogeneity in strawberry stolon buds. Results: From observation, we found that the axillary bud located in the first node quickly stepped into dormancy (DSB), after several bract and leaf buds were differentiated. The stolon apical meristem (SAM) degenerated as the new ramet leaf buds (RLB), and the new active axillary stolon buds (ASB) differentiated continually after the differentiation of the first leaf. Using the tandem mass tags (TMT) labeling method, a total of 7,271 strawberry proteins were identified. Between ASB and DSB, the spliceosome DEPs, such as Ser/Arg-rich (SR) and heterogeneous nuclear ribonucleoprotein particle (hnRNP), showed the highest enrichment and high PPI connectivity. This indicated that the differences in DEPs (e.g., SF-3A and PK) at the transcriptional level may be causing the differences between the physiological statuses of ASB and DSB. As expected, the photosynthetic pre-form RLB mainly differentiated from ASB and DSB judging by the DEP enrichment of photosynthesis. However, there are still other specialized features of DEPs between RLB and DSB and between ASB and DSB. The DEPs relative to DNA duplication [e.g., minichromosome maintenance protein (MCM 2, 3, 4, 7)], provide a strong hint of functional gene duplication leading the bud heterogeneity between RLB and DSB. In addition, the top fold change DEP of LSH 10-like might be involved in the degeneration of SAM into RLBs, based on its significant function in modulating the plant shoot initiation. As for RLB/ASB, the phenylpropanoid biosynthesis pathway probably regulates the ramet axillary bud specialization, and further promotes the differentiation of xylem when ASB develops into a new stolon [e.g., cinnamyl alcohol dehydrogenase 1 (CAD1) and phenylalanine ammonia-lyase 1 (PAL1)]. Conclusions: By using phenotypic observation combined with proteomic networks with different types of strawberry stolon buds, the definite dormancy phase of DSB was identified, and the biological pathways and gene networks that might be responsible for heterogeneity among different stolon buds in strawberry were also revealed.

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