scholarly journals Quantitative Proteomics Analysis of Altered Protein Expression in the Placental Villous Tissue of Early Pregnancy Loss Using Isobaric Tandem Mass Tags

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Xiaobei Ni ◽  
Xin Li ◽  
Yueshuai Guo ◽  
Tao Zhou ◽  
Xuejiang Guo ◽  
...  
Keyword(s):  
Protein Expression ◽  
Pregnant Women ◽  
Early Pregnancy ◽  
Proteomic Analysis ◽  
Pregnancy Loss ◽  
Tandem Mass ◽  
Pathophysiological Mechanism ◽  
Early Pregnancy Loss ◽  
Expression Levels ◽  
Tandem Mass Tags

Many pregnant women suffer miscarriages during early gestation, but the description of these early pregnancy losses (EPL) can be somewhat confusing because of the complexities of early development. Thus, the identification of proteins with different expression profiles related to early pregnancy loss is essential for understanding the comprehensive pathophysiological mechanism. In this study, we report a gel-free tandem mass tags- (TMT-) labeling based proteomic analysis of five placental villous tissues from patients with early pregnancy loss and five from normal pregnant women. The application of this method resulted in the identification of 3423 proteins and 19647 peptides among the patient group and the matched normal control group. Qualitative and quantitative proteomic analysis revealed 51 proteins to be differentially abundant between the two groups (≥1.2-fold, Student'st-test,P<0.05). To obtain an overview of the biological functions of the proteins whose expression levels altered significantly in EPL group, gene ontology analysis was performed. We also investigated the twelve proteins with a difference over 1.5-fold using pathways analysis. Our results demonstrate that the gel-free TMT-based proteomic approach allows the quantification of differences in protein expression levels, which is useful for obtaining molecular insights into early pregnancy loss.

scholarly journals Proteomic Analysis on the Alteration of Protein Expression in the Placental Villous Tissue of Early Pregnancy Loss

2006 ◽  
Vol 75 (3) ◽  
pp. 414-420 ◽  
Author(s):  
Ai-Xia Liu ◽  
Fan Jin ◽  
Wu-Wen Zhang ◽  
Tian-Hua Zhou ◽  
Cai-Yun Zhou ◽  
...  
Keyword(s):  
Protein Expression ◽  
Early Pregnancy ◽  
Proteomic Analysis ◽  
Pregnancy Loss ◽  
Early Pregnancy Loss ◽  
Villous Tissue

scholarly journals EFFECTIVENESS OF METFORMIN IN PREVENTING EARLY PREGNANCY LOSS IN WOMEN WITH POLYCYSTIC OVARIAN SYNDROME

2019 ◽  
pp. 1-4
Author(s):  
Deepali Janugade
Keyword(s):  
Pregnant Women ◽  
Early Pregnancy ◽  
Pregnancy Loss ◽  
Polycystic Ovary ◽  
Free Testosterone ◽  
Serum Glucose ◽  
Control Group ◽  
Early Pregnancy Loss ◽  
Metformin Therapy ◽  
Metformin Group

OBJECTIVE : To evaluate the effectiveness of metformin therapy in preventing early pregnancy loss in pregnant women with polycystic ovary syndrome (PCOS). MATERIALS AND METHODS : This is a prospective cohort study conducted in the Obstetric Department of Krishna Institute of Medical Sciences, Karad, Maharashtra, India for a period of 2 years. This study involved 100 nondiabetic pregnant women with PCOS. They were divided into two groups, namely, the group that received metformin throughout pregnancy (metformin group) and the group that got pregnant but, did not receive metformin (control group). A comparison was made between the two groups of patients with respect to certain basal characteristics (age, body mass index, previous obstetric outcome, serum glucose with free testosterone). Statistical analysis was performed using Chi-square test to compare the differences between the two groups. RESULTS : There were 50 patients who received metformin during pregnancy (metformin group) compared with 50 patients who did not receive the treatment (control group). The rate of early pregnancy loss in the metformin group was 10% (5/50) compared with 36% (18/50) in the control group (p < 0.001). For patients in the metformin group with a history of previous miscarriage, the rate of pregnancy loss was 45% (35 cases/50 pregnancies). CONCLUSION : Metformin therapy in pregnant women with PCOS was associated with a significant reduction in the rate of early pregnancy loss

scholarly journals Oxidative Stress Markers in Early Pregnancy Loss: A Case-Control Study

2018 ◽  
Vol 7 (1) ◽  
pp. 61-66 ◽  
Author(s):  
Shahnaz Torkzahrani ◽  
Padideh Janati Ataei ◽  
Mehdi Hedayati ◽  
Soheila Khodakarim ◽  
Zohre Sheikhan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Uric Acid ◽  
Pregnant Women ◽  
Spontaneous Abortion ◽  
Early Pregnancy ◽  
Pregnancy Loss ◽  
First Trimester ◽  
Demographic Characteristics ◽  
Early Pregnancy Loss ◽  
First Trimester Of Pregnancy

Objectives: Evidence suggests that oxidative stress (OS) plays a prominent role in the pathophysiology of pregnancy complications in women. The present study was conducted to determine the levels of OS markers in early pregnancy loss and to compare the results with those in healthy pregnant women. Materials and Methods: A total of 32 women with early pregnancy loss and 32 healthy women in the first trimester of pregnancy, with similar demographic characteristics entered this study as the cases and controls. Serum levels of malondialdehyde (MDA), total antioxidant capacity (TAC), uric acid, and bilirubin levels were determined in both groups. The data obtained were then analyzed and compared between the groups using the independent samples t test and Mann-Whitney U test. Results: The 2 groups matched in terms of personal-demographic characteristics including mother’s age, father’s age, gravidity, and body mass index (BMI). MDA levels increased significantly in the women with spontaneous abortion compared to the healthy pregnant women (4.35±1.47 vs. 3.42±1.68 µM/L; P=0.026) and TAC decreased significantly in the cases compared to the healthy controls (552.34±212.79 vs. 1003.23±1168.68 U/mL; P=0.040). Uric acid and bilirubin levels did not differ between the groups. Conclusions: The results of this study provides further evidence on the effect of increased OS on the incidence of early spontaneous abortion in the first trimester of pregnancy. High serum MDA levels and low TAC during pregnancy were 2 risk factors for spontaneous abortion. The present findings support the hypothesis that OS plays a key role in the etiopathogenesis of spontaneous abortion. Further studies are required for assessing the preventive role of antioxidant therapy in this complication.

scholarly journals How does subchorionic hematoma in the first trimester affect pregnancy outcomes?

2021 ◽  
Author(s):  
Taner Günay ◽  
Oğuz Yardımcı
Keyword(s):  
Pregnant Women ◽  
Preterm Delivery ◽  
Early Pregnancy ◽  
Pregnancy Outcomes ◽  
Vaginal Bleeding ◽  
Pregnancy Loss ◽  
Placental Abruption ◽  
First Trimester ◽  
Early Pregnancy Loss ◽  
Adverse Pregnancy

IntroductionSubchorionic hematoma (SCH) in pregnancy has been associated with increased risk of adverse pregnancy outcomes. We aimed to investigate the association of SCH with adverse pregnancy outcomes in pregnant women in relation to size of hematoma and control subjects.Material and methodsThis study included 178 pregnant women with sonographically detected SCH in the 1st trimester, and 350 pregnant controls without SCH. Data on maternal age, smoking status, gestational week at diagnosis, location of SCH, medications before diagnosis, gestational week at delivery, delivery route and pregnancy outcomes (first trimester vaginal bleeding, pre-eclampsia, gestational diabetes, intrauterine growth restriction (IUGR), placental abruption, preterm delivery < 37 weeks, early pregnancy loss and intrauterine death) were retrieved retrospectively from hospital records. Pregnant women with SCH were divided into 3 groups according to the size of hematoma including small SCH (SCH-I group, n = 47), medium- size SCH (SCH-II group, n = 110) and large SCH (SCH-III group, n = 21) groups.ResultsSubchorionic hematoma was associated with significantly lower gestational age at delivery (p < 0.001) and higher rate of first trimester bleeding (p < 0.001) compared with the control group, regardless of the size of the hematoma. Placental abruption (p = 0.002) and early pregnancy loss (p < 0.001) were significantly more common in SCH-II and -III groups than in the control group. SCH-III group was associated with a significantly higher rate of < 37 gestational weeks at delivery (p < 0.001), first trimester vaginal bleeding (p < 0.001), early pregnancy loss (p < 0.001), IUGR (p = 0.003) and preterm delivery (p < 0.001) compared to both lesser size hematoma and control groups.ConclusionsIn conclusion, our findings suggest that large SCH might indicate an increased risk of adverse pregnancy outcomes such as 1st trimester vaginal bleeding, early pregnancy loss, IUGR, placental abruption or preterm delivery. These findings are important to guide the patients with SCH for detailed clinical evaluation.

scholarly journals Use of serum leptin and insulin hormones levels as predictors of pregnancy outcome in pregnant women with history of recurrent early pregnancy loss

2016 ◽  
Vol 21 (1) ◽  
pp. 36-40
Author(s):  
Ayman Abd-Elaziz El-Dorf ◽  
Ahmed Mohamed Eid Ossman ◽  
Ahmed El-Sayed El-Halwagy ◽  
Hesham Mohamed Borg ◽  
Enaam Salah Abd-ElBar
Keyword(s):  
Pregnant Women ◽  
Pregnancy Outcome ◽  
Early Pregnancy ◽  
Pregnancy Loss ◽  
Early Pregnancy Loss ◽  
Serum Leptin ◽  
History Of ◽  
Recurrent Early Pregnancy Loss

scholarly journals Serum Progesterone and Serial Beta-hcG Levels in Predicting the Outcome of Early Pregnancies With Doubtful Viability: A Prospective Observational Research

2021 ◽  
Author(s):  
Mustafa YILDIZ ◽  
Esra Nur CICEK ◽  
Gokhan DEMIRAYAK ◽  
Orhan SAHIN ◽  
Yagmur OLMEZ ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Early Pregnancy ◽  
Pregnancy Loss ◽  
Progesterone Level ◽  
Observational Research ◽  
City Hospital ◽  
Early Pregnancy Loss ◽  
Intrauterine Pregnancy ◽  
Serum Progesterone ◽  
Β Hcg

Abstract BACKGROUND: To evaluate the doubling rate of maternal serum serial β-hCG and a single initial serum progesterone level to predict fetal viability before ultrasonography in women diagnosed with intrauterine pregnancy of undetectable viability. METHODS: 336 pregnant women who applied to the outpatient clinic at Istanbul Okmeydanı City Hospital between March and December 2018 were evaluated on a “Prospective observational” basis. 236 women were excluded from the study for various reasons. The study was completed with 100 pregnant women diagnosed with intrauterine pregnancy (IUP) involving suspected fetal viability by transvaginal ultrasonography only, who met the inclusion criteria with CRL: < 7mm and mean gestational sac diameter: < 25mm. Serum β-hCG and progesterone were taken at the first admission. After 48 hours, control serum β-hCG was taken and the increase rates were calculated. Early pregnancy loss was diagnosed by (transvaginal) TV-USG performed after the days 7, 11 and 14. Patients were divided into two groups as fetal heart rate (FHR) positive (viable) and FHR negative (early pregnancy loss). Pregnancy results were compared with β-hCG increase rates and progesterone value. SPSS 22.0 software was used for statistical analysis and P<0.05 was accepted as statistically significant.RESULTS: No statistically significant result was obtained between the viable and early pregnancy loss (FHR +/-) groups in terms of maternal age, previous pregnancy anamnesis, nationality, presenting symptoms, or ultrasound findings. According to the last menstrual period, the mean gestational age was 45.1±14 days in the viable (FHR+) group and 51.3±14 days in the Early pregnancy loss (FHR-) group, and this difference was found to be statistically significant. Estimation modality was developed in terms of viability with the serum progesterone values and increase rates of β-hCG. The study, which was conducted with a confidence interval of 95%, found the viability rate to be 70% with a β-hCG increase rate of 31%, 80% in the case of an increase by 49%, 90% in the case of an increase by 73%, 95% in the case of an increase by 97%, and 100% in the case of an increase by 181%. For progesterone, when the value was 5.9 ng/ml, the viability rate was 49%, and it was 69% at 10.5 ng/ml, 80% at 13.4 ng/ml, 90% at 18.0 ng/ml, 95% at 21.7 ng/ml, 99% at 29.3 ng/ml, and 100% at 37.5 ng/ml and above. In conclusion, the significant efficacy values of β-hCG increase and first progesterone level in predicting viability were found to be ROC AUC: [0.748 (0.621-0.874)] and ROC AUC: [0.796 (0.685-0.907)], respectively.CONCLUSION: Either Serial β-hCG ratio or serum progesterone level can be used alone to predict pregnancy outcome in early pregnancy. With dissemination of similar studies, estimation modalities can be improved and TV-USG examinations can help shortening the waiting time for results to reduce anxiety of families, hospital admissions and health expenses.

scholarly journals Non-typeable Haemophilus influenzae-associated early pregnancy loss: an emerging neonatal and maternal pathogen

Infection ◽  
2019 ◽  
Vol 48 (2) ◽  
pp. 285-288
Author(s):  
Muge Cevik ◽  
Olga L. Moncayo-Nieto ◽  
Margaret J. Evans
Keyword(s):  
Abdominal Pain ◽  
Haemophilus Influenzae ◽  
Pregnant Women ◽  
Early Pregnancy ◽  
Pregnancy Loss ◽  
Significant Proportion ◽  
Previous History ◽  
Clinical Signs ◽  
Early Pregnancy Loss ◽  
Septic Abortion

Abstract Objectives There is increasing evidence indicating an association between invasive non-typeable Haemophilus influenzae (NTHi) infection in pregnancy and early pregnancy loss. As the diagnosis relies on microbiological investigation of post-mortem placental and foetal samples, a significant proportion of NTHi-related pregnancy loss remains unrecognised. To better characterise NTHi in septic abortion, we report NTHi cases associated with early pregnancy loss. Methods We reviewed all post-mortems at <24 weeks gestation with histologically proven acute chorioamnionitis on placental histology and enrolled cases with at least one matched foetal and placental sample culture positive for NTHi. The study was approved by the NHS Lothian Caldicott Guardian. Results In our cohort, invasive NTHi has accounted for 20% of infections associated with early pregnancy loss prior to 24 weeks gestation. All patients were young and healthy pregnant women at < 20 weeks' gestation who presented with abdominal pain, PV bleed /discharge and were septic at the time of presentation. One patient with previous history of miscarriage who presented with cervical incompetence had more severe pathology suggestive of early intrauterine pneumonia. Conclusion The burden of invasive NTHi disease in early pregnancy loss is likely to be much larger than currently recognised. NTHi should be considered in pregnant women presenting with abdominal pain and PV bleed/discharge in whom clinical signs of sepsis are present. Active surveillance should be considered in this patient group including septic abortion to capture the true prevalence of this emerging pathogen to inform preventative and therapeutic approaches.

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