scholarly journals Direct Quantification of MicroRNA at Low Picomolar Level in Sera of Glioma Patients Using a Competitive Hybridization Followed by Amplified Voltammetric Detection

2012 ◽  
Vol 84 (15) ◽  
pp. 6400-6406 ◽  
Author(s):  
Jianxiu Wang ◽  
Xinyao Yi ◽  
Hailin Tang ◽  
Hongxing Han ◽  
Minghua Wu ◽  
...  
Keyword(s):  
Voltammetric Detection ◽  
Competitive Hybridization ◽  
Direct Quantification

Voltammetric Detection of Tetrodotoxin Real-Time In Vivo of Mouse Organs using DNA-Immobilized Carbon Nanotube Sensors

2019 ◽  
Vol 15 (5) ◽  
pp. 567-574
Author(s):  
Huck Jun Hong ◽  
Suw Young Ly
Keyword(s):  
Carbon Nanotube ◽  
Real Time ◽  
Anticancer Agents ◽  
Cancer Metastasis ◽  
Anodic Stripping Voltammetry ◽  
Stripping Voltammetry ◽  
Takifugu Rubripes ◽  
In Vivo Detection ◽  
Voltammetric Detection

Background: Tetrodotoxin (TTX) is a biosynthesized neurotoxin that exhibits powerful anticancer and analgesic abilities by inhibiting voltage-gated sodium channels that are crucial for cancer metastasis and pain delivery. However, for the toxin’s future medical applications to come true, accurate, inexpensive, and real-time in vivo detection of TTX remains as a fundamental step. Methods: In this study, highly purified TTX extracted from organs of Takifugu rubripes was injected and detected in vivo of mouse organs (liver, heart, and intestines) using Cyclic Voltammetry (CV) and Square Wave Anodic Stripping Voltammetry (SWASV) for the first time. In vivo detection of TTX was performed with auxiliary, reference, and working herring sperm DNA-immobilized carbon nanotube sensor systems. Results: DNA-immobilization and optimization of amplitude (V), stripping time (sec), increment (mV), and frequency (Hz) parameters for utilized sensors amplified detected peak currents, while highly sensitive in vivo detection limits, 3.43 µg L-1 for CV and 1.21 µg L-1 for SWASV, were attained. Developed sensors herein were confirmed to be more sensitive and selective than conventional graphite rodelectrodes modified likewise. A linear relationship was observed between injected TTX concentration and anodic spike peak height. Microscopic examination displayed coagulation and abnormalities in mouse organs, confirming the powerful neurotoxicity of extracted TTX. Conclusion: These results established the diagnostic measures for TTX detection regarding in vivo application of neurotoxin-deviated anticancer agents and analgesics, as well as TTX from food poisoning and environmental contamination.

scholarly journals Direct Quantification of Heat Generation Due to Inelastic Scattering of Electrons Using a Nanocalorimeter

2020 ◽  
pp. 2002876
Author(s):  
Joonsuk Park ◽  
Kiho Bae ◽  
Taeho Roy Kim ◽  
Christopher Perez ◽  
Aditya Sood ◽  
...  
Keyword(s):  
Inelastic Scattering ◽  
Heat Generation ◽  
Direct Quantification

Drug Preconcentration and Direct Quantification in Biofluids Using 3D-Printed Paper Cartridge

2021 ◽  
pp. 113266
Author(s):  
He Chen ◽  
Langlang Yi ◽  
Jianduo Wu ◽  
Guoqian Li ◽  
Gang Zhao ◽  
...  
Keyword(s):  
3D Printed ◽  
Direct Quantification

Normality Ranges of Menstrual Fluid Volume During Reproductive Life Using Direct Quantification of Menses with Vaginal Cups

2019 ◽  
Vol 84 (4) ◽  
pp. 390-395 ◽  
Author(s):  
Manuel B. Donoso ◽  
Ramón Serra ◽  
Gregory E. Rice ◽  
María Teresa Gana ◽  
Carolina Rojas ◽  
...  
Keyword(s):  
Fluid Volume ◽  
Reproductive Life ◽  
Direct Quantification ◽  
Menstrual Fluid

Porous silicon membrane-modified electrodes for label-free voltammetric detection of MS2 bacteriophage

2016 ◽  
Vol 80 ◽  
pp. 47-53 ◽  
Author(s):  
Nekane Reta ◽  
Andrew Michelmore ◽  
Christopher Saint ◽  
Beatriz Prieto-Simón ◽  
Nicolas H. Voelcker
Keyword(s):  
Porous Silicon ◽  
Modified Electrodes ◽  
Label Free ◽  
Silicon Membrane ◽  
Ms2 Bacteriophage ◽  
Voltammetric Detection

Molecular Speciated Isotope Dilution Mass Spectrometric Methods for Accurate, Reproducible and Direct Quantification of Reduced, Oxidized and Total Glutathione in Biological Samples

2015 ◽  
Vol 87 (2) ◽  
pp. 1232-1240 ◽  
Author(s):  
Timothy Fahrenholz ◽  
Mesay Mulugeta Wolle ◽  
H. M. “Skip” Kingston ◽  
Scott Faber ◽  
John C. Kern ◽  
...  
Keyword(s):  
Isotope Dilution ◽  
Biological Samples ◽  
Mass Spectrometric ◽  
Total Glutathione ◽  
Direct Quantification
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