scholarly journals Development and Certification of a Standard Reference Material for Vitamin D Metabolites in Human Serum

2011 ◽  
Vol 84 (2) ◽  
pp. 956-962 ◽  
Author(s):  
Karen W. Phinney ◽  
Mary Bedner ◽  
Susan S.-C. Tai ◽  
Veronica V. Vamathevan ◽  
Lane C. Sander ◽  
...  
Keyword(s):  
Vitamin D ◽  
Reference Material ◽  
Human Serum ◽  
Standard Reference Material ◽  
Vitamin D Metabolites

Development of Standard Reference Material (SRM) 2973 Vitamin D Metabolites in Frozen Human Serum (High Level)

2017 ◽  
Vol 100 (5) ◽  
pp. 1294-1303 ◽  
Author(s):  
Susan S.-C. Tai ◽  
Michael A. Nelson ◽  
Mary Bedner ◽  
Brian E. Lang ◽  
Karen W. Phinney ◽  
...  
Keyword(s):  
Vitamin D ◽  
Reference Material ◽  
Human Serum ◽  
Standard Reference Material ◽  
Vitamin D Metabolites ◽  
High Level

scholarly journals Development of an Improved Standard Reference Material for Vitamin D Metabolites in Human Serum

2017 ◽  
Vol 89 (9) ◽  
pp. 4907-4913 ◽  
Author(s):  
Karen W. Phinney ◽  
Susan S.-C. Tai ◽  
Mary Bedner ◽  
Johanna E. Camara ◽  
Rosalind R. C. Chia ◽  
...  
Keyword(s):  
Vitamin D ◽  
Reference Material ◽  
Human Serum ◽  
Standard Reference Material ◽  
Vitamin D Metabolites

scholarly journals Certification of standard reference material 968f fat-soluble vitamins in frozen human serum

2017 ◽  
Author(s):  
Jeanice Brown Thomas ◽  
David L Duewer ◽  
Carolyn Q Burdette ◽  
Lorna T Sniegoski ◽  
James H Yen
Keyword(s):  
Reference Material ◽  
Human Serum ◽  
Standard Reference Material ◽  
Fat Soluble Vitamins

Certification of drugs of abuse in a human serum standard reference material: SRM 1959

2010 ◽  
Vol 397 (2) ◽  
pp. 501-509 ◽  
Author(s):  
Susan S.-C. Tai ◽  
Jocelyn L. Prendergast ◽  
Lorna T. Sniegoski ◽  
Michael J. Welch ◽  
Karen W. Phinney ◽  
...  
Keyword(s):  
Reference Material ◽  
Human Serum ◽  
Standard Reference Material ◽  
Drugs Of Abuse

Preparation and value assignment of standard reference material 968e fat-soluble vitamins, carotenoids, and cholesterol in human serum

2011 ◽  
Vol 402 (2) ◽  
pp. 749-762 ◽  
Author(s):  
Jeanice B. Thomas ◽  
David L. Duewer ◽  
Isaac O. Mugenya ◽  
Karen W. Phinney ◽  
Lane C. Sander ◽  
...  
Keyword(s):  
Reference Material ◽  
Human Serum ◽  
Standard Reference Material ◽  
Value Assignment ◽  
Fat Soluble Vitamins

Simultaneous measurement of 13 circulating vitamin D3 and D2 mono and dihydroxy metabolites using liquid chromatography mass spectrometry

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Carl Jenkinson ◽  
Reena Desai ◽  
Andrzej T. Slominski ◽  
Robert C. Tuckey ◽  
Martin Hewison ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Vitamin D ◽  
Liquid Chromatography ◽  
Human Serum ◽  
High Sensitivity ◽  
Reference Level ◽  
Vitamin D Metabolites ◽  
Serum Samples ◽  
Human Serum Samples ◽  
Limit Of Quantitation

Abstract Objectives Clinical evaluation of vitamin D status is conventionally performed by measuring serum levels of a single vitamin D metabolite, 25-hydroxyvitamin D predominantly by immunoassay methodology. However, this neglects the complex metabolic pathways involved in vitamin D bioactivity, including two canonical forms D3 and D2, bioactive 1,25-dihydroxy metabolites and inactive 24-hydroxy and other metabolites. Methods Liquid chromatography-tandem mass spectrometry (LC-MS/MS) can measure multiple analytes in a sample during a single run with high sensitivity and reference level specificity. We therefore aimed to develop and validate a LC-MS/MS method to measure simultaneously 13 circulating vitamin D metabolites and apply it to 103 human serum samples. Results The LC-MS/MS method using a Cookson-type derivatization reagent phenyl-1,2,4-triazoline-3,5-dione (PTAD) quantifies 13 vitamin D metabolites, including mono and dihydroxy-metabolites, as well as CYP11A1-derived D3 and D2 metabolites in a single run. The lower limit of quantitation was 12.5 pg/mL for 1,25(OH)2D3 with accuracy verified by analysis of National Institute of Standards and Technology (NIST) 972a standards. Quantification of seven metabolites (25(OH)D3, 25(OH)D2, 3-epi-25(OH)D3, 20(OH)D3, 24,25(OH)2D3, 1,25(OH)2D3 and 1,20S(OH)2D3) was consistently achieved in human serum samples. Conclusions This profiling method can provide new insight into circulating vitamin D metabolite pathways forming the basis for improved understanding of the role of vitamin D in health and disease.

Do we measure bilirubin correctly anno 2005?

2005 ◽  
Vol 43 (5) ◽  
Author(s):  
Joke J. Apperloo ◽  
Fedde van der Graaf ◽  
Volkher Scharnhorst ◽  
Huib L. Vader
Keyword(s):  
Reference Material ◽  
Human Serum ◽  
Reference Materials ◽  
Standard Reference Material ◽  
Total Bilirubin ◽  
Reference Method ◽  
Serum Samples ◽  
Suitable Material ◽  
Good Agreement

AbstractWe observed 30% discrepancy between liquid chemistry and dry chemistry analysers for the determination of total bilirubin in human adult serum samples, which were consistent with a 20% overestimation and 10% underestimation relative to a Jendrassik-Grof reference method, respectively. In contrast, standard reference material SRM916, which was recently recommended as being the most suitable material for attaining interlaboratory agreement, shows very good agreement on both types of analysers, as well as close to 100% recovery with respect to the reference method. We show that the liquid vs. dry bilirubin discrepancies seem to originate in the presence of either conjugated or δ-bilirubin. Our observations make it clear that good interlaboratory (or inter-analyser) agreement between bilirubin reference materials does not guarantee the same for bilirubin concentrations in human serum samples.

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