scholarly journals LC-MS/MS Methods for Absolute Quantification and Identification of Proteins Associated with Chimeric Plant Oil Bodies

2011 ◽  
Vol 83 (24) ◽  
pp. 9267-9272 ◽  
Author(s):  
Floriana Capuano ◽  
Nicholas J. Bond ◽  
Laurent Gatto ◽  
Frédéric Beaudoin ◽  
Johnathan A. Napier ◽  
...  
Author(s):  
Jia Hao ◽  
Duoxia Xu ◽  
Yangping Cao

Oil bodies (OBs) are micron- or submicron-sized sub-organelles widely found in plants seeds and nuts. The structure OBs is composed of a core of triglycerides covered by a phospholipid-protein layer, which ensures the stability of the OBs under extreme environmental conditions and further protects core lipids as energy reserves. As naturally pre-emulsified oil-in-water emulsions, OBs have been gradually applied to replace synthetically engineered oil droplets. In this paper, the recent research on the composition, extraction, stability, delivery system, digestion, food applications and future perspectives of plant OBs are reviewed. Recent studies have focused on the OBs surface protein identification and function, large-scale extraction techniques such as enzyme assisted, high pressure, ultrasound, and extrusion and the reconstituted OBs. Electrostatic deposition of polysaccharides significantly improves the stability of OBs emulsions. OBs emulsions have promising applications to encapsulate bioactive compounds, deliver targeted drugs, and prepare gels and edible functional films. The digestive behavior of OBs emulsions is similar to that of protein-stabilized emulsions, which can increase the satiety, effectively help reduce calorie intake and improve the bioavailability of functional factors. It has also promoted the development of simulated dairy, spices and meat products.


1996 ◽  
Vol 31 (5) ◽  
pp. 945-956 ◽  
Author(s):  
Johnathan A. Napier ◽  
A. Keith Stobart ◽  
Peter R. Shewry
Keyword(s):  

2014 ◽  
Vol 406 (20) ◽  
pp. 4921-4929 ◽  
Author(s):  
Ting Tan ◽  
Chang-jiang-sheng Lai ◽  
Su-ling Zeng ◽  
E-Hu Liu ◽  
Ping Li

2010 ◽  
Vol 163 (6) ◽  
pp. 792-802 ◽  
Author(s):  
Stefania Bonsegna ◽  
Simona Bettini ◽  
Rosanna Pagano ◽  
Antonella Zacheo ◽  
Viviana Vergaro ◽  
...  
Keyword(s):  

ISRN Botany ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-16 ◽  
Author(s):  
Jason T. C. Tzen

Hydrophobic storage neutral lipids are stably preserved in specialized organelles termed oil bodies in the aqueous cytosolic compartment of plant cells via encapsulation with surfactant molecules including phospholipids and integral proteins. To date, three classes of integral proteins, termed oleosin, caleosin, and steroleosin, have been identified in oil bodies of angiosperm seeds. Proposed structures, targeting traffic routes, and biological functions of these three integral oil-body proteins were summarized and discussed. In the viewpoint of evolution, isoforms of oleosin and caleosin are found in oil bodies of pollens as well as those of more primitive species; moreover, caleosin- and steroleosin-like proteins are also present in other subcellular locations besides oil bodies. Technically, artificial oil bodies of structural stability similar to native ones were successfully constituted and seemed to serve as a useful tool for both basic research studies and biotechnological applications.


2018 ◽  
Vol 44 (2) ◽  
pp. 56-65
Author(s):  
M. E. Bondarchuk ◽  
◽  
V. V. Kozlova ◽  

2015 ◽  
Vol 11 (1) ◽  
Author(s):  
Zulaicha Dwi Hastuti ◽  
Dwi Husodo Prasetyo ◽  
Erlan Rosyadi
Keyword(s):  
Palm Oil ◽  

Asam lemak bebas (alb) dalam Crude Palm Oil (CPO) merupakan salah satu parameter kualitas CPO.Munculnya asamlemak bebas ini dapat berasal dari faktor pemanenan dan penyimpanan. Asamlemak bebas yang tinggi dalam CPO dapat menurunkan harga CPO. Minyak mentah sawit inimerupakan salah satu sumber energi terbarukan yang diolah, antara lain, menjadi pure plant oil (PPO)dan biodiesel. Namun,masalah utama CPO sebagai bahan baku PPO dan biodiesel adalah asamlemak bebas. Asam lemak bebas dalam CPO yang dapat digunakan untuk PPO dan biodiesel tidakboleh lebih dari 1%. Oleh karena itu, dalam penelitian ini dilakukan usaha untuk menurunkan asamlemak bebas dalam CPO sehingga dapat dimanfaatkan sebagai bahan bakar. Metoda yang digunakanadalah esterifikasi. Asam lemak bebas dikonversi menjadi metil ester. Campuran metil ester dengantrigliserida ini merupakan bahan bakar yang dapat digunakan untuk pengganti BBM. Kondisi yangoptimal untuk proses esterifikasi CPO alb tinggi adalah sebagai berikut : suhu 65oC, waktu 360 menit,katalis 0,25%, dan perbandingan mol metanol 8:1. Dengan proses ini, asam lemak bebas dapatditurunkan menjadi 2,76% (konversi 89,39%). Pada kondisi yang sama dengan katalis yangditingkatkan menjadi 0,5%, asam lemak bebas dapat diturunkan menjadi 1,86% (konversi 92,85%).Pada kondisi yang sama, dengan katalis yang ditingkatkan menjadi 1%, asam lemak bebas dapatditurunkan menjadi 1,75% (konversi 93,28%).Kata kunci: asam lemak bebas, CPO, esterifikasi, PPO, biodiesel


2018 ◽  
Author(s):  
Rudolf Fullybright

Accurate quantification of biological resistance has been impossible so far. Among the various forms of biological resistance which exist in nature, pathogen resistance to drugs is a familiar one. However, as in the case of other forms of resistance, accurately quantifying drug resistance in pathogens has been impossible up to now. Here, we introduce a mathematically-defined and uniform procedure for the absolute quantification of biological resistance deployed by any living organism in the biological realm, including and beyond drug resistance in medicine. The scheme introduced makes possible the exact measurement or computation of the extent to which resistance is deployed by any living organism regardless of kingdom and regardless of the mechanism of resistance involved. Furthermore, the Second Law of Resistance indicating that resistance has the potential to increase to infinite levels, and the Third Law of Resistance indicating that resistance comes to an end once interaction stops, the resistance unit function introduced here is fully compatible with both the Second and Third Laws of Resistance.


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