scholarly journals Regenerable and Simultaneous Surface Plasmon Resonance Detection of Aβ(1−40) and Aβ(1−42) Peptides in Cerebrospinal Fluids with Signal Amplification by Streptavidin Conjugated to an N-Terminus-Specific Antibody

2010 ◽  
Vol 82 (24) ◽  
pp. 10151-10157 ◽  
Author(s):  
Ning Xia ◽  
Lin Liu ◽  
Michael G. Harrington ◽  
Jianxiu Wang ◽  
Feimeng Zhou
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Specific Antibody ◽  
Plasmon Resonance ◽  
Signal Amplification ◽  
N Terminus ◽  
Resonance Detection

Surface plasmon resonance detection of small molecule using split aptamer fragments

2011 ◽  
Vol 156 (2) ◽  
pp. 893-898 ◽  
Author(s):  
Qing Wang ◽  
Jiahao Huang ◽  
Xiaohai Yang ◽  
Kemin Wang ◽  
Leiliang He ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Small Molecule ◽  
Plasmon Resonance ◽  
Resonance Detection

scholarly journals Surface plasmon resonance biosensor for exosome detection based on reformative tyramine signal amplification activated by molecular aptamer beacon

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Wenqin Chen ◽  
Zhiyang Li ◽  
Wenqian Cheng ◽  
Tao Wu ◽  
Jia Li ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Signal Amplification ◽  
Lipid Membrane ◽  
Breast Cancer Patients ◽  
Her2 Positive ◽  
Chip Surface ◽  
Spr Biosensor ◽  
Aptamer Beacon

AbstractHuman epidermal growth factor receptor 2 (HER2)-positive exosomes play an extremely important role in the diagnosis and treatment options of breast cancers. Herein, based on the reformative tyramine signal amplification (TSA) enabled by molecular aptamer beacon (MAB) conversion, a label-free surface plasmon resonance (SPR) biosensor was proposed for highly sensitive and specific detection of HER2-positive exosomes. The exosomes were captured by the HER2 aptamer region of MAB immobilized on the chip surface, which enabled the exposure of the G-quadruplex DNA (G4 DNA) that could form peroxidase-like G4-hemin. In turn, the formed G4-hemin catalyzed the deposition of plentiful tyramine-coated gold nanoparticles (AuNPs-Ty) on the exosome membrane with the help of H2O2, generating a significantly enhanced SPR signal. In the reformative TSA system, the horseradish peroxidase (HRP) as a major component was replaced with nonenzymic G4-hemin, bypassing the defects of natural enzymes. Moreover, the dual-recognition of the surface proteins and lipid membrane of the desired exosomes endowed the sensing strategy with high specificity without the interruption of free proteins. As a result, this developed SPR biosensor exhibited a wide linear range from 1.0 × 104 to 1.0 × 107 particles/mL. Importantly, this strategy was able to accurately distinguish HER2-positive breast cancer patients from healthy individuals, exhibiting great potential clinical application. Graphical Abstract

Grating-based surface plasmon resonance detection of core-shell nanoparticle mediated DNA hybridization

2012 ◽  
Vol 32 (1) ◽  
pp. 141-147 ◽  
Author(s):  
Seyoung Moon ◽  
Yonghwi Kim ◽  
Youngjin Oh ◽  
Hosub Lee ◽  
Hyun Chang Kim ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Dna Hybridization ◽  
Core Shell ◽  
Resonance Detection
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