scholarly journals Label-Free Porous Silicon Immunosensor for Broad Detection of Opiates in a Blind Clinical Study and Results Comparison to Commercial Analytical Chemistry Techniques

2010 ◽  
Vol 82 (23) ◽  
pp. 9711-9718 ◽  
Author(s):  
Lisa M. Bonanno ◽  
Tai C. Kwong ◽  
Lisa A. DeLouise
Keyword(s):  
Analytical Chemistry ◽  
Clinical Study ◽  
Porous Silicon ◽  
Label Free

Porous silicon membrane-modified electrodes for label-free voltammetric detection of MS2 bacteriophage

2016 ◽  
Vol 80 ◽  
pp. 47-53 ◽  
Author(s):  
Nekane Reta ◽  
Andrew Michelmore ◽  
Christopher Saint ◽  
Beatriz Prieto-Simón ◽  
Nicolas H. Voelcker
Keyword(s):  
Porous Silicon ◽  
Modified Electrodes ◽  
Label Free ◽  
Silicon Membrane ◽  
Ms2 Bacteriophage ◽  
Voltammetric Detection

Design and realization of label-free optical immunosensor based on porous silicon microcavities

2008 ◽  
Author(s):  
Xiao-yi Lü ◽  
Tao Xue ◽  
Zhen-hong Jia ◽  
Hua-wei Shao ◽  
Shi-bin Hou ◽  
...  
Keyword(s):  
Porous Silicon ◽  
Label Free ◽  
Optical Immunosensor

scholarly journals Label-Free Virus Capture and Release by a Microfluidic Device Integrated with Porous Silicon Nanowire Forest

Small ◽  
2016 ◽  
Vol 13 (6) ◽  
pp. 1603135 ◽  
Author(s):  
Yiqiu Xia ◽  
Yi Tang ◽  
Xu Yu ◽  
Yuan Wan ◽  
Yizhu Chen ◽  
...  
Keyword(s):  
Porous Silicon ◽  
Microfluidic Device ◽  
Silicon Nanowire ◽  
Label Free ◽  
Free Virus ◽  
Capture And Release ◽  
Porous Silicon Nanowire

scholarly journals A lectin-coupled porous silicon-based biosensor: label-free optical detection of bacteria in a real-time mode

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Mona Yaghoubi ◽  
Fereshteh Rahimi ◽  
Babak Negahdari ◽  
Ali Hossein Rezayan ◽  
Azizollah Shafiekhani
Keyword(s):  
Fourier Transform ◽  
Porous Silicon ◽  
Real Time ◽  
Limit Of Detection ◽  
Principal Component ◽  
The Other ◽  
Detection Methods ◽  
Carbohydrate Binding ◽  
Label Free ◽  
E Coli

Abstract Accuracy and speed of detection, along with technical and instrumental simplicity, are indispensable for the bacterial detection methods. Porous silicon (PSi) has unique optical and chemical properties which makes it a good candidate for biosensing applications. On the other hand, lectins have specific carbohydrate-binding properties and are inexpensive compared to popular antibodies. We propose a lectin-conjugated PSi-based biosensor for label-free and real-time detection of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) by reflectometric interference Fourier transform spectroscopy (RIFTS). We modified meso-PSiO2 (10–40 nm pore diameter) with three lectins of ConA (Concanavalin A), WGA (Wheat Germ Agglutinin), and UEA (Ulex europaeus agglutinin) with various carbohydrate specificities, as bioreceptor. The results showed that ConA and WGA have the highest binding affinity for E. coli and S. aureus respectively and hence can effectively detect them. This was confirmed by 6.8% and 7.8% decrease in peak amplitude of fast Fourier transform (FFT) spectra (at 105 cells mL−1 concentration). A limit of detection (LOD) of about 103 cells mL−1 and a linear response range of 103 to 105 cells mL−1 were observed for both ConA-E. coli and WGA-S. aureus interaction platforms that are comparable to the other reports in the literature. Dissimilar response patterns among lectins can be attributed to the different bacterial cell wall structures. Further assessments were carried out by applying the biosensor for the detection of Klebsiella aerogenes and Bacillus subtilis bacteria. The overall obtained results reinforced the conjecture that the WGA and ConA have a stronger interaction with Gram-positive and Gram-negative bacteria, respectively. Therefore, it seems that specific lectins can be suggested for bacterial Gram-typing or even serotyping. These observations were confirmed by the principal component analysis (PCA) model.

scholarly journals Label-free discrimination of membrane-translocating peptides on porous silicon microfluidic biosensors

2016 ◽  
Vol 10 (6) ◽  
pp. 064113 ◽  
Author(s):  
Zhen Li ◽  
Qiaohui Luo ◽  
Jianmin Wu
Keyword(s):  
Porous Silicon ◽  
Label Free
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