Verification of Intramolecular Hybrid/Parallel G-Quadruplex Structure under Physiological Conditions Using Novel Cyanine Dye H-Aggregates: Both in Solution and on Au Film

2010 ◽  
Vol 82 (22) ◽  
pp. 9135-9137 ◽  
Author(s):  
Qianfan Yang ◽  
Jun-Feng Xiang ◽  
Shu Yang ◽  
Qian Li ◽  
Qiuju Zhou ◽  
...  
Keyword(s):  
Cyanine Dye ◽  
Physiological Conditions ◽  
Au Film ◽  
Quadruplex Structure ◽  
G Quadruplex

Reversible regulation of the supramolecular chirality of a cyanine dye by using the G-quadruplex structure as a template

2016 ◽  
Vol 52 (45) ◽  
pp. 7302-7305 ◽  
Author(s):  
Yunhua Shi ◽  
Hongxia Sun ◽  
Junfeng Xiang ◽  
Hongbo Chen ◽  
Suge Zhang ◽  
...  
Keyword(s):  
Cyanine Dye ◽  
Quadruplex Structure ◽  
Supramolecular Chirality ◽  
G Quadruplex ◽  
Multiple Cycle ◽  
Cycle Regulation

Multiple cycle regulation of the supramolecular chirality of a cyanine dye has been successfully achieved by using DNA G-quadruplexes as templates, which is easily controllable by repeated addition of Ag+ and cysteine (Cys).

scholarly journals The parallel G-quadruplex structure of vertebrate telomeric repeat sequences is not the preferred folding topology under physiological conditions

2011 ◽  
Vol 39 (13) ◽  
pp. 5768-5775 ◽  
Author(s):  
Robert Hänsel ◽  
Frank Löhr ◽  
Silvie Foldynová-Trantírková ◽  
Ernst Bamberg ◽  
Lukáš Trantírek ◽  
...  
Keyword(s):  
Telomeric Repeat ◽  
Physiological Conditions ◽  
Quadruplex Structure ◽  
Repeat Sequences ◽  
G Quadruplex

scholarly journals A novel G-quadruplex motif in the Human MET promoter region

2017 ◽  
Vol 37 (6) ◽  
Author(s):  
Jing Yan ◽  
Deming Zhao ◽  
Liping Dong ◽  
Shuang Pan ◽  
Fengjin Hao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Regulation ◽  
Luciferase Assay ◽  
Klenow Fragment ◽  
Regulate Gene Expression ◽  
Physiological Conditions ◽  
Quadruplex Structure ◽  
G Quadruplex ◽  
Quadruplex Formation

It is known that the guanine-rich strands in proto-oncogene promoters can fold into G-quadruplex structures to regulate gene expression. An intramolecular parallel G-quadruplex has been identified in MET promoter. It acts as a repressor in regulating MET expression. However, the full guanine-rich region in MET promoter forms a hybrid parallel/antiparallel G-quadruplex structure under physiological conditions, which means there are some antiparallel and hybrid parallel/antiparallel G-quadruplex structures in this region. In the present study, our data indicate that g3-5 truncation adopts an intramolecular hybrid parallel/antiparallel G-quadruplex under physiological conditions in vitro. The g3-5 G-quadruplex structure significantly stops polymerization by Klenow fragment in K+ buffer. Furthermore, the results of circular dichroism (CD) spectra and polymerase stop assay directly demonstrate that the G-quadruplex structure in g3-5 fragment can be stabilized by the G-quadruplex ligand TMPyP4 (5,10,15,20-tetra-(N-methyl-4-pyridyl) porphine). But the dual luciferase assay indicates TMPyP4 has no effect on the formation of g3-5 G-quadruplex in HepG2 cells. The findings in the present study will enrich our understanding of the G-quadruplex formation in proto-oncogene promoters and the mechanisms of gene expression regulation.

A cyanine dye supramolecular FRET switch driven by G-quadruplex to monitor mitophagy

2021 ◽  
pp. 109429
Author(s):  
Xiaomeng Guo ◽  
Dawei Yang ◽  
Ranran Sun ◽  
Qian Li ◽  
Hongyan Du ◽  
...  
Keyword(s):  
Cyanine Dye ◽  
G Quadruplex
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