scholarly journals Characterization of Murine Brain Membrane Glycoproteins by Detergent Assisted Lectin Affinity Chromatography

2010 ◽  
Vol 82 (15) ◽  
pp. 6329-6333 ◽  
Author(s):  
Xin Wei ◽  
Charles Dulberger ◽  
Lingjun Li
Keyword(s):  
Affinity Chromatography ◽  
Membrane Glycoproteins ◽  
Lectin Affinity Chromatography ◽  
Brain Membrane ◽  
Lectin Affinity ◽  
Murine Brain

Antibodies Against Platelet Membrane Glycoproteins: Effect On Ristocetin-Induced Platelet Aggregation

1981 ◽  
Author(s):  
E F Ali-Briggs ◽  
C S P Jenkins ◽  
K J Clemetson
Keyword(s):  
Platelet Aggregation ◽  
Affinity Chromatography ◽  
Platelet Membrane ◽  
Receptor Activity ◽  
Free Medium ◽  
Membrane Glycoproteins ◽  
Lectin Affinity Chromatography ◽  
Lectin Affinity ◽  
Fab Fragments ◽  
Induced Aggregation

Some membrane glycoproteins (GPs) have been isolated by lectin-affinity chromatography and antibodies towards them have been raised. Platelets that have lost glycocalicin no longer respond to ristocetin-human VIII:WF, bovine VIIIR:WF, or to anti-glycocalicin or anti-GPs la and lb antibodies but are still agglutinated by anti-GPs lib and Ilia antibodies. Anti-GPs la and lb and anti-glycocalicin antibodies, IgG and Fab' fragments inhibited ristocetin- human VIIIR:WF- and bovine VIIIR:WF-induced aggregation of fixed, washed platelets and of platelets in plasma while anti-GPs Hb and Ilia antibodies were without effect.Crossed immunoelectrophorectic studies showed that glycocalicin was present on whole platelets in only trace amounts; anti-glycocalicin antibodies, however, recognized a slower migrating component. Platelets incubated in an EDTA-free medium no longer respond to ristocetin-human VIIIRrWF. Membranes isolated from such platelets contained glycocalicin which cross-reacted with a remnant of the slower migrating component. Anti-GPs la and lb antibodies gave more complex patterns but it was possible to identify the slower moving component recognized by the anti-glycocalicin antibodies.These results show that glycocalicin is not normally found as such on whole platelets but is present as a precursor which is most likely GP lb. On degradation of this precursor, glycocalicin is released from the membrane and VIIIRrWF-receptor activity is lost.

scholarly journals Characterization of Glycoproteins in Pancreatic Cyst Fluid Using a High-Performance Multiple Lectin Affinity Chromatography Platform

2013 ◽  
Vol 13 (1) ◽  
pp. 289-299 ◽  
Author(s):  
Francisca Owusu Gbormittah ◽  
Brian B. Haab ◽  
Katie Partyka ◽  
Carolina Garcia-Ott ◽  
Marina Hancapie ◽  
...  
Keyword(s):  
Affinity Chromatography ◽  
High Performance ◽  
Pancreatic Cyst ◽  
Cyst Fluid ◽  
Lectin Affinity Chromatography ◽  
Lectin Affinity ◽  
Pancreatic Cyst Fluid
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