Comparative Study of Random and Oriented Antibody Immobilization Techniques on the Binding Capacity of Immunosensor

2010 ◽  
Vol 82 (15) ◽  
pp. 6401-6408 ◽  
Author(s):  
A. Kausaite-Minkstimiene ◽  
A. Ramanaviciene ◽  
J. Kirlyte ◽  
A. Ramanavicius
Keyword(s):  
Comparative Study ◽  
Binding Capacity ◽  
Antibody Immobilization ◽  
Immobilization Techniques

A COMPARATIVE STUDY OF PREPARATIONS OF OVINE LUTEINIZING HORMONE BY BIOASSAY, IMMUNODOUBLEDIFFUSION, RADIOIMMUNOASSAY, RADIORECEPTOR ASSAY AND POLYACRYLAMIDE GEL ELECTROPHORESIS

1976 ◽  
Vol 81 (2) ◽  
pp. 270-282 ◽  
Author(s):  
M. Schlamowitz ◽  
J. Cronquist ◽  
M. Esfahani ◽  
D. N. Ward
Keyword(s):  
Luteinizing Hormone ◽  
Comparative Study ◽  
Gel Electrophoresis ◽  
Binding Capacity ◽  
Polyacrylamide Gel Electrophoresis ◽  
Radioreceptor Assay ◽  
Polyacrylamide Gel ◽  
Polyacrylamide Gels ◽  
Biological Potency

ABSTRACT Three preparations of ovine LH were compared for biological potency and by several in vitro parameters. All were found to be heterogenous by immunodoublediffusion and by electrophoresis in polyacrylamide gels. They all also showed similarities and/or differences with respect to their characteristics in immunodoublediffusion, radioimmunoassay, radioreceptor assay, gel electrophoresis and in dye-binding capacity, but in ways that preclude establishing a meaningful correlation between biopotency and the in vitro parameters or even among the in vitro parameters themselves. The implications of these findings for the use of these in vitro parameters for screening and assessing biological potencies of LH preparations and for inferring chemical and/or structural similarities between LH preparations are discussed. Aspects of polymorphism of LH, observed by electrophoresis on polyacrylamide gels, are also discussed.

Graphene Synthesis and Antibody Immobilization Techniques for Immunosensors

2020 ◽  
pp. 21-34
Author(s):  
Ihda Uswatun Shalihah Shohibuddin ◽  
Piravin Raj Barthasarathy ◽  
Wan Wardatul Amani Wan Salim
Keyword(s):  
Antibody Immobilization ◽  
Graphene Synthesis ◽  
Immobilization Techniques

A comparative study on antibody immobilization strategies onto solid surface

2013 ◽  
Vol 30 (10) ◽  
pp. 1934-1938 ◽  
Author(s):  
Ji Eun Lee ◽  
Jeong Hyun Seo ◽  
Chang Sup Kim ◽  
Yunkyeoung Kwon ◽  
Jeong Hyub Ha ◽  
...  
Keyword(s):  
Comparative Study ◽  
Solid Surface ◽  
Antibody Immobilization

scholarly journals Comparison of Oriented and Random Antibody Immobilization Techniques on the Efficiency of Immunosensor

2012 ◽  
Vol 47 ◽  
pp. 837-840 ◽  
Author(s):  
J. Baniukevic ◽  
J.Kirlyte ◽  
A. Ramanavicius ◽  
A. Ramanaviciene
Keyword(s):  
Antibody Immobilization ◽  
Immobilization Techniques

scholarly journals Comparative study of antibody immobilization mediated by lipid and polymer fibers

2015 ◽  
Vol 134 ◽  
pp. 1-7 ◽  
Author(s):  
Celine Cohn ◽  
Siu Ling Leung ◽  
Zhengbao Zha ◽  
Jessica Crosby ◽  
Weibing Teng ◽  
...  
Keyword(s):  
Comparative Study ◽  
Polymer Fibers ◽  
Antibody Immobilization

scholarly journals Binding Characteristics Study of DNA based Aptamers for E. coli O157:H7

Molecules ◽  
2021 ◽  
Vol 26 (1) ◽  
pp. 204
Author(s):  
Saika Siddiqui ◽  
Jie Yuan
Keyword(s):  
Comparative Study ◽  
Binding Affinity ◽  
Rapid Detection ◽  
Binding Capacity ◽  
Comprehensive Analysis ◽  
Binding Characteristics ◽  
E Coli ◽  
Fast Detection ◽  
The Common ◽  
Binding Capability

E. coli O157:H7 is a pathogenic bacterium producing verotoxins that could lead to serious complications such as hemolytic uremia syndrome. Fast detection of such pathogens is important. For rapid detection, aptamers are quickly gaining traction as alternative biorecognition molecules besides conventional antibodies. Several DNA aptamers have been selected for E. coli O157:H7. Nonetheless, there has not been a comparative study of the binding characteristics of these aptamers. In this work, we present a comprehensive analysis of binding characteristics including binding affinity (Kd) and binding capacity (Bmax) of DNA-based aptamers for E. coli O157:H7 using qPCR. Our results show that aptamer E18R has the highest binding capacity to E. coli 157:H7 and the highest specificity over non-pathogenic E. coli strains K12 and DH5α. Our study also finds that the common biotin-tag modification at 5′ end typically changes the binding capacity significantly. For most of the selected aptamers, the binding capacity after a biotin-tag modification decreases. There exists a discrepancy in the binding capability between the selected aptamer and the aptamer used for detection. Our study also shows that a lower concentration of Mg2+ ions in the binding buffer leads to a decrease in the binding capacity of E17F and E18R, while it does not affect the binding capacity of S1 and EcoR1.

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