Human Urine as Test Material in1H NMR-Based Metabonomics:  Recommendations for Sample Preparation and Storage

2007 ◽  
Vol 79 (3) ◽  
pp. 1181-1186 ◽  
Author(s):  
Michael Lauridsen ◽  
Steen H. Hansen ◽  
Jerzy W. Jaroszewski ◽  
Claus Cornett
Keyword(s):  
Sample Preparation ◽  
Human Urine ◽  
Test Material ◽  
And Storage

New sample preparation method to analyse 15 specific and non-specific pesticide metabolites in human urine using LC-MS/MS

2021 ◽  
Vol 1166 ◽  
pp. 122542
Author(s):  
Petra Stuchlík Fišerová ◽  
Jiří Kohoutek ◽  
Céline Degrendele ◽  
Mohamed Aqiel Dalvie ◽  
Jana Klánová
Keyword(s):  
Sample Preparation ◽  
Human Urine ◽  
Preparation Method ◽  
Sample Preparation Method ◽  
Pesticide Metabolites

scholarly journals Assessment of human plasma and urine sample preparation for reproducible and high-throughput UHPLC-MS clinical metabolic phenotyping

The Analyst ◽  
2020 ◽  
Vol 145 (20) ◽  
pp. 6511-6523 ◽  
Author(s):  
Andrew D. Southam ◽  
Liam D. Haglington ◽  
Lukáš Najdekr ◽  
Andris Jankevics ◽  
Ralf J. M. Weber ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Human Plasma ◽  
Urine Sample ◽  
High Throughput ◽  
Human Urine ◽  
Metabolic Phenotyping ◽  
Preparation Methods ◽  
Human Plasma And Urine ◽  
Multiple Sample ◽  
Sample Preparation Methods

In this study we assess multiple sample preparation methods for UHPLC-MS metabolic phenotyping analysis of human urine and plasma. All methods are discussed in terms of metabolite and lipid coverage and reproducibility.

An HPLC/ICPMS study of the stability of selenosugars in human urine: implications for quantification, sample handling, and storage

2006 ◽  
Vol 21 (7) ◽  
pp. 684-690 ◽  
Author(s):  
Dijana Juresa ◽  
Jérôme Darrouzès ◽  
Norbert Kienzl ◽  
Maité Bueno ◽  
Florence Pannier ◽  
...  
Keyword(s):  
Human Urine ◽  
Sample Handling ◽  
The Stability ◽  
And Storage

Empfehlungen zur qualitätsgesicherten Durchführung von Nukleinsäure-Amplifikations - Testungen (NAT) von Blutprodukten. Quality assurance in nucleic acid amplification testing (NAT) of blood products

2005 ◽  
Vol 29 (2) ◽  
Author(s):  
Willi K. Roth
Keyword(s):  
Quality Control ◽  
Nucleic Acid ◽  
Sample Preparation ◽  
Nucleic Acids ◽  
Internal Control ◽  
Control Measures ◽  
Nucleic Acid Amplification ◽  
Blood Products ◽  
Whole Process ◽  
And Storage

AbstractEuropean manufacturers of plasma products and German blood transfusion services were the first to introduce nucleic acid amplification testing (NAT) of blood products in the mid-1990s. Their primary goal was to increase the safety of blood by closing as far as possible the diagnostic window, which exists after the onset of viral infection until the appearance of the first detectable antibodies. Sample preparation, transport and storage are crucial steps in a quality-controlled PCR. Sensitivity and contamination rates highly depend on the sample preparation and storage techniques. Anticoagulants must be selected carefully because some may inhibit the PCR. Dilution of samples by pooling needs to be considered and should be compensated for by subsequent virus enrichment procedures, e.g. centrifugation. The whole process of sample preparation, pooling and virus enrichment must be validated and quality control measures must be implemented. Reagents for the extraction of viral nucleic acids should not pose any risk to the laboratory staff. Nevertheless, the reagents should be highly efficient in liberating viral nucleic acids at high yield and purity for the following amplification reactions. At this critical stage, quality control measures should guarantee an efficient extraction process and contain potential sources of contaminations. Several methods are available for the amplification of nucleic acids. PCR is the most common, especially in in-house assays. The amplification of nucleic acids should be performed as far as possible in a closed system, which may be guaranteed best by real-time PCR approaches. Reaction tubes need never be opened during the amplification because detection can be performed through the closed tube. Amplicons that could contaminate the following PCR reactions will not be released. It is of great importance to blood transfusion services to guarantee that negative results un-equivocally indicate virus negative blood donations. Therefore, internal control sequences should be implemented in each individual PCR reaction in order to monitor that the individual PCR has worked correctly. Besides internal control sequences, external negative and positive controls should be implemented in each PCR run to demonstrate false positive reactions as well as to monitor pre-PCR processes like virus enrichment and extraction. The whole process needs to be validated according to the criteria set in national guidelines or by national authorities. External quality assessment programs are highly recommended.

The Importance of Sample Preparation and Storage in Glutathione Analysis

1993 ◽  
Vol 211 (2) ◽  
pp. 183-187 ◽  
Author(s):  
J.C. Roberts ◽  
D.J. Francetic
Keyword(s):  
Sample Preparation ◽  
And Storage
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