Fatty Acid Analysis Tool (FAAT):  An FT-ICR MS Lipid Analysis Algorithm

2006 ◽  
Vol 78 (15) ◽  
pp. 5497-5503 ◽  
Author(s):  
Michael D. Leavell ◽  
Julie A. Leary
Keyword(s):  
Fatty Acid ◽  
Lipid Analysis ◽  
Fatty Acid Analysis ◽  
Analysis Tool ◽  
Analysis Algorithm ◽  
Ft Icr Ms ◽  
Ft Icr

Dual-polarity SALDI FT-ICR MS imaging and Kendrick mass defect data filtering for lipid analysis

2020 ◽  
Author(s):  
Wendy H. Müller ◽  
Alexandre Verdin ◽  
Christopher Kune ◽  
Johann Far ◽  
Edwin De Pauw ◽  
...  
Keyword(s):  
Lipid Analysis ◽  
Mass Defect ◽  
Data Filtering ◽  
Ms Imaging ◽  
Kendrick Mass Defect ◽  
Dual Polarity ◽  
Ft Icr Ms ◽  
Ft Icr

scholarly journals A comparison of two common sample preparation techniques for lipid and fatty acid analysis in three different coral morphotypes reveals quantitative and qualitative differences

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3645 ◽  
Author(s):  
Jessica A. Conlan ◽  
Melissa M. Rocker ◽  
David S. Francis
Keyword(s):  
Fatty Acid ◽  
Sample Preparation ◽  
Lipid Analysis ◽  
Lipid Extraction ◽  
Fatty Acid Analysis ◽  
Preparation Technique ◽  
Pocillopora Damicornis ◽  
Physiological Processes ◽  
Coral Holobiont ◽  
Preparation Techniques

Lipids are involved in a host of biochemical and physiological processes in corals. Therefore, changes in lipid composition reflect changes in the ecology, nutrition, and health of corals. As such, accurate lipid extraction, quantification, and identification is critical to obtain comprehensive insight into a coral’s condition. However, discrepancies exist in sample preparation methodology globally, and it is currently unknown whether these techniques generate analogous results. This study compared the two most common sample preparation techniques for lipid analysis in corals: (1) tissue isolation by air-spraying and (2) crushing the coral in toto. Samples derived from each preparation technique were subsequently analysed to quantify lipids and their constituent classes and fatty acids in four common, scleractinian coral species representing three distinct morphotypes (Acropora millepora, Montipora crassotuberculata, Porites cylindrica, and Pocillopora damicornis). Results revealed substantial amounts of organic material, including lipids, retained in the skeletons of all species following air-spraying, causing a marked underestimation of total lipid concentration using this method. Moreover, lipid class and fatty acid compositions between the denuded skeleton and sprayed tissue were substantially different. In particular, the majority of the total triacylglycerol and total fatty acid concentrations were retained in the skeleton (55–69% and 56–64%, respectively). As such, the isolated, sprayed tissue cannot serve as a reliable proxy for lipid quantification or identification in the coral holobiont. The in toto crushing method is therefore recommended for coral sample preparation prior to lipid analysis to capture the lipid profile of the entire holobiont, permitting accurate diagnoses of coral condition.

PHYTOCHEMICAL SCREENING, ANTIOXIDANT, ANTI-CYTOTOXIC AND ANTICANCER EFFECTS OF GALINSOGA PARVIFLORA AND VERNONIA POLYANTHES (ASTERACEAE) EXTRACTS

2020 ◽  
Vol 8 (10) ◽  
pp. 84-98
Author(s):  
Sula M. V. Feleti ◽  
Renê L. Aleluia ◽  
Suiany V. Gervásio ◽  
Jean Carlos V. Dutra ◽  
Jessica R. P. Oliveira ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Phenolic Acids ◽  
Unsaturated Fatty Acids ◽  
Biological Effects ◽  
Human Lymphocytes ◽  
Chemical Characterization ◽  
Total Content ◽  
Ags Cells ◽  
Ft Icr Ms ◽  
Ft Icr

The study was designed to investigate the chemical composition and the biological effects of G. parviflora and V. polyanthes ethanolic extracts in vitro. Total content of phenols, flavonoids and tannins was quantified by spectrophotometry; chemical characterization was permed by mass spectrometry (ESI (-) FT-ICR MS and APCI (+) FT-ICR MS analysis). Antioxidant activities were determined by FRAP and Fe2+ chelating methods. Extracts cytotoxicity was evaluated in human lymphocytes, sarcoma-180 (S-180) and human gastric adenocarcinoma (AGS) cells, by MTT assay. V. polyanthes presented higher total content of tannins and G. parviflora presented higher amount of phenols and flavonoids. Chemical characterization showed the presence of flavonoids, phenolic acids and sesquiterpene lactones in V. polyanthes extract, and steroids, phenolic acids and fatty acids (Poly Unsaturated Fatty Acids - PUFA) in G. parviflora extract. V. polyanthes extract stood out in the Fe2+ chelation test. G. parviflora extract did not present outstanding antioxidant results in the tested protocols. Both species showed a tendency to promote cytotoxicity in human lymphocyte cells. Regarding the antiproliferative effect, both species were able to reduce S-180 cell viability and G. parviflora extract showed high antiproliferative potential in the assay with AGS cells. These findings reinforce the medicinal use of these plants, as well as suggest their potential use for the development of new drugs and for the treatment of cancers.

Chemical Constituents in Some Promising Genotypes of Indian Mustard [Brassica juncea (L.) Czern and Coss.]

2017 ◽  
Vol 4 (04) ◽  
Author(s):  
SUNITA SINGH ◽  
R. P. SINGH ◽  
H. K. SINGH ◽  
N. A. KHAN ◽  
M. K. MAURYA
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Erucic Acid ◽  
Brassica Juncea ◽  
Acid Composition ◽  
Chemical Constituents ◽  
Indian Mustard ◽  
Acid Value ◽  
Fatty Acid Analysis ◽  
Crude Fibre

Among the oilseed Brassica crops, Indian mustard [Brassica juncea (L.) Czern and Coss.] is an important source of oil from a nutritional point of view. The nutritional value of oil and cake quality is governed mainly by the composition of its fatty acids, iodine value, saponification, acid value, glucosinolates, crude fibre, protein and limiting amino acids, etc. Seventeen varieties/strains of Indian mustard were taken for saturated and unsaturated fatty acid analysis. The eicosenoic was absent in genotype (NUDBYJ-10) and erucic acid (NUDBYJ-10, LES-46 and Pusa mustard- 21). The fatty acid composition found a variable in different genotypes. Saturated fatty acid, Palmitic + Stearic ranged between 2.3 to 6.5%, Oleic 10.6 to 40.7%, Linoleic 16.1 to 37.7%, Linolenic 13.3 to 26.7%, Eicosenoic 0.00 to 10.30% and Erucic acid 0.00 to 47.50%, respectively. Alternaria blight severity also varied in different genotypes and ranged between 18.75 to 56.25%, maximum being in genotype Kranti and minimum in LES-47. No significant correlation was observed between the fatty acid composition and disease severity. The oil content range from 38.1 to 42.60% and protein content was found highest in variety RGN-73. The amino acid viz. methionine and tryptophan range between 0.41 to 1.81 g/16gN and 0.41 to 1.81 g /16g N, respectively.

EVLUETION OF DIETARY UTILIZATION OF BLOOM-FORMING CYANOBACTERIA BY SMALL-BODIED CRUSTACEAN ZOOPLANKTON USING FATTY ACID ANALYSIS

2019 ◽  
Vol 75 (7) ◽  
pp. III_367-III_374
Author(s):  
Souta AOMORI ◽  
Megumu FUJIABAYASHI ◽  
Kunihiro OKANO ◽  
Yoshihiro TAKADA ◽  
Naoyuki MIYATA
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Analysis ◽  
Crustacean Zooplankton

scholarly journals Lipid Metabolism Is Dysregulated before, during and after Pregnancy in a Mouse Model of Gestational Diabetes

2021 ◽  
Vol 22 (14) ◽  
pp. 7452
Author(s):  
Samuel Furse ◽  
Denise S. Fernandez-Twinn ◽  
Davide Chiarugi ◽  
Albert Koulman ◽  
Susan E. Ozanne
Keyword(s):  
Lipid Metabolism ◽  
Gestational Diabetes ◽  
Glucose Tolerance ◽  
Mouse Model ◽  
Time Course ◽  
De Novo ◽  
Lipid Analysis ◽  
Temporal Distribution ◽  
De Novo Lipogenesis ◽  
Analysis Tool

The aim of the current study was to test the hypothesis that maternal lipid metabolism was modulated during normal pregnancy and that these modulations are altered in gestational diabetes mellitus (GDM). We tested this hypothesis using an established mouse model of diet-induced obesity with pregnancy-associated loss of glucose tolerance and a novel lipid analysis tool, Lipid Traffic Analysis, that uses the temporal distribution of lipids to identify differences in the control of lipid metabolism through a time course. Our results suggest that the start of pregnancy is associated with several changes in lipid metabolism, including fewer variables associated with de novo lipogenesis and fewer PUFA-containing lipids in the circulation. Several of the changes in lipid metabolism in healthy pregnancies were less apparent or occurred later in dams who developed GDM. Some changes in maternal lipid metabolism in the obese-GDM group were so late as to only occur as the control dams’ systems began to switch back towards the non-pregnant state. These results demonstrate that lipid metabolism is modulated in healthy pregnancy and the timing of these changes is altered in GDM pregnancies. These findings raise important questions about how lipid metabolism contributes to changes in metabolism during healthy pregnancies. Furthermore, as alterations in the lipidome are present before the loss of glucose tolerance, they could contribute to the development of GDM mechanistically.

Application of frequency multiple FT-ICR-MS signal acquisition for improved proteome research

2021 ◽  
pp. 116578
Author(s):  
Sung-Gun Park ◽  
Jared P. Mohr ◽  
Gordon A. Anderson ◽  
James E. Bruce
Keyword(s):  
Signal Acquisition ◽  
Ft Icr Ms ◽  
Ft Icr
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