On-Column Coaxial Flow Chemiluminescence Detection for Underivatized Amino Acids by Pressurized Capillary Electrochromatography Using a Monolithic Column

2006 ◽  
Vol 78 (15) ◽  
pp. 5322-5328 ◽  
Author(s):  
Zian Lin ◽  
Zenghong Xie ◽  
Haixia Lü ◽  
Xucong Lin ◽  
Xiaoping Wu ◽  
...  
Keyword(s):  
Amino Acids ◽  
Capillary Electrochromatography ◽  
Monolithic Column ◽  
Chemiluminescence Detection ◽  
Pressurized Capillary Electrochromatography ◽  
Underivatized Amino Acids

Polymethacryloyl-L-Phenylalanine [PMAPA]-Based Monolithic Column for Capillary Electrochromatography

2019 ◽  
Vol 57 (8) ◽  
pp. 758-765 ◽  
Author(s):  
Ali Derazshamshir ◽  
Süleyman Aşır ◽  
Ilgım Göktürk ◽  
Sisem Ektirici ◽  
Fatma Yılmaz ◽  
...  
Keyword(s):  
Amino Acids ◽  
Capillary Electrochromatography ◽  
Hydrophobic Interactions ◽  
Monolithic Column ◽  
Applied Pressure ◽  
Chemical Species ◽  
Analytical Techniques ◽  
Research Focus ◽  
Analytical Technique ◽  
Short Period

Abstract The ability to detect catecholamines (CAs) and their metabolites is vital to understand the mechanism behind the neuronal diseases. Neurochemistry aims to provide an improved pharmacological, molecular and physiological understanding of complex brain chemistries by analytical techniques. Capillary electrophoresis (CE) is one such analytical technique that enables the study of various chemical species ranging from amino acids and peptides to natural products and drugs. CE can easily adapt the changes in research focus and in recent years remains an applicable technique for investigating neuroscience and single cell neurobiology. The prepared phenylalanine-based hydrophobic monolithic column, Polymethacryloyl-L-phenylalanine [PMAPA], was used as a stationary phase in capillary electrochromatography to separate CAs that are similar in size and shape to each other including dopamine (DA) and norepinephrine (NE) via hydrophobic interactions. Separation carried out in a short period of 17 min was performed with the electrophoretic mobility of 5.54 × 10−6 m2 V−1 s−1 and 7.60 × 10−6 m2 V−1 s−1 for DA and NE, respectively, at pH 7.0, 65% acetonitrile ratio with 100 mbar applied pressure by the developed hydrophobic monolithic column without needing any extra process such as imprinting or spacer arms to immobilize ligands used in separation.

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