Electrochromatographic Enantioseparation of Amino Acids Using Polybutylmethacrylate-based Chiral Monolithic Column by Capillary Electrochromatography

Chirality ◽  
2012 ◽  
Vol 24 (8) ◽  
pp. 606-609 ◽  
Author(s):  
Cemil Aydogan ◽  
Adil Denizli
Keyword(s):  
Amino Acids ◽  
Capillary Electrochromatography ◽  
Monolithic Column

Polymethacryloyl-L-Phenylalanine [PMAPA]-Based Monolithic Column for Capillary Electrochromatography

2019 ◽  
Vol 57 (8) ◽  
pp. 758-765 ◽  
Author(s):  
Ali Derazshamshir ◽  
Süleyman Aşır ◽  
Ilgım Göktürk ◽  
Sisem Ektirici ◽  
Fatma Yılmaz ◽  
...  
Keyword(s):  
Amino Acids ◽  
Capillary Electrochromatography ◽  
Hydrophobic Interactions ◽  
Monolithic Column ◽  
Applied Pressure ◽  
Chemical Species ◽  
Analytical Techniques ◽  
Research Focus ◽  
Analytical Technique ◽  
Short Period

Abstract The ability to detect catecholamines (CAs) and their metabolites is vital to understand the mechanism behind the neuronal diseases. Neurochemistry aims to provide an improved pharmacological, molecular and physiological understanding of complex brain chemistries by analytical techniques. Capillary electrophoresis (CE) is one such analytical technique that enables the study of various chemical species ranging from amino acids and peptides to natural products and drugs. CE can easily adapt the changes in research focus and in recent years remains an applicable technique for investigating neuroscience and single cell neurobiology. The prepared phenylalanine-based hydrophobic monolithic column, Polymethacryloyl-L-phenylalanine [PMAPA], was used as a stationary phase in capillary electrochromatography to separate CAs that are similar in size and shape to each other including dopamine (DA) and norepinephrine (NE) via hydrophobic interactions. Separation carried out in a short period of 17 min was performed with the electrophoretic mobility of 5.54 × 10−6 m2 V−1 s−1 and 7.60 × 10−6 m2 V−1 s−1 for DA and NE, respectively, at pH 7.0, 65% acetonitrile ratio with 100 mbar applied pressure by the developed hydrophobic monolithic column without needing any extra process such as imprinting or spacer arms to immobilize ligands used in separation.

Analysis of diuretics by capillary electrochromatography using poly(1-hexadecene-co-TMPTMA) monolithic column

2010 ◽  
Vol 28 (3) ◽  
pp. 253-259 ◽  
Author(s):  
Minghua LU ◽  
Xin LI ◽  
Qiang FENG ◽  
Guonan CHEN ◽  
Lan ZHANG
Keyword(s):  
Capillary Electrochromatography ◽  
Monolithic Column

Ionic-Liquid-Modified, Hybrid Silica Monolithic Column for Capillary Electrochromatography

2011 ◽  
Vol 39 (6) ◽  
pp. 827-832 ◽  
Author(s):  
Rui FENG ◽  
Min SHEN ◽  
Ming-Ming WANG ◽  
Hao CHEN ◽  
Zhao-Rui ZENG
Keyword(s):  
Ionic Liquid ◽  
Capillary Electrochromatography ◽  
Monolithic Column ◽  
Hybrid Silica

Simultaneous determination of nucleoside and purine compounds in human urine based on a hydrophobic monolithic column using capillary electrochromatography

2015 ◽  
Vol 36 (21-22) ◽  
pp. 2727-2735 ◽  
Author(s):  
Zongbao Chen ◽  
Chuanghui Huang ◽  
Wei Liu ◽  
Lin Zhang ◽  
Ping Tong ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Human Urine ◽  
Capillary Electrochromatography ◽  
Monolithic Column
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