Direct Measurement of Free Chloride Concentrations in the Phagolysosomes of Human Neutrophils

2006 ◽  
Vol 78 (9) ◽  
pp. 3133-3137 ◽  
Author(s):  
Richard G. Painter ◽  
Guoshun Wang
Keyword(s):  
Direct Measurement ◽  
Human Neutrophils ◽  
Free Chloride ◽  
Chloride Concentrations

Direct Measurement of Chloride in Sweat with an Ion-Selective Electrode

1973 ◽  
Vol 19 (7) ◽  
pp. 727-730 ◽  
Author(s):  
LaVerne Szabo, Margaret ◽  
A Kenny ◽  
Winnie Lee
Keyword(s):  
Direct Measurement ◽  
Chloride Concentration ◽  
Ion Selective Electrode ◽  
Selective Electrode ◽  
Sweat Chloride ◽  
Insufficient Amount ◽  
Chloride Concentrations

Abstract The use of a specific ion electrode for measuring sweat chloride has not been widely accepted because of poor precision and occasional falsely elevated chloride concentrations. We have studied this technique by using the Orion sweat-chloride instrument, and have eliminated these problems by modifying the methodology. An insufficient amount of sweat for determination with the electrode was responsible for both the imprecision and random inaccuracies. The following modifications eliminated these problems: (a) We freshly saturate felt pads with a solution of, per deciliter, 400 mg of pilocarpine nitrate and 1 g of NaHCO3 to replace the electrolyte pads supplied by the Orion Co. (b) The timed iontophoresis period commences after the meter indicates conduction of adequate current. (c) After iontophoresis, sweat is collected for 10 min under a plastic cap held over the induction area. Then chloride concentration is measured with the electrode. With these improvements in technique, the revised procedure should be as reliable as reference methodologies.

Neutrophil Myeloperoxidase Destruction by Ultraviolet Irradiation

1988 ◽  
Vol 46 ◽  
pp. 138-139
Author(s):  
J. Hanker ◽  
B. Giammara ◽  
G. Strauss
Keyword(s):  
Uv Radiation ◽  
Myeloid Leukemia ◽  
Stratospheric Ozone ◽  
Psoriasis Patient ◽  
Whole Body ◽  
Human Neutrophils ◽  
Cupric Nitrate ◽  
The Earth ◽  
Routine Handling ◽  
Uvb Phototherapy

Only a fraction of the UV radiation emitted by the sun reaches the earth; most of the UVB (290-320nm) is eliminated by stratospheric ozone. There is increasing concern, however, that man-made chemicals are damaging this ozone layer. Although the effects of UV on DNA or as a carcinogen are widely known, preleukemia and acute myeloid leukemia (AML) have only rarely been reported in psoriasis patients treated with 8-methoxypsoralen and UV (PUVA). It was therefore of interest to study the effects of UV on the myeloperoxidase (MP) activity of human neutrophils. The peroxidase activity of enriched leukocyte preparations on coverslips was shown cytochemically with a diaminobenzidine medium and cupric nitrate intensification.Control samples (Figs. 1,4,5) of human bloods that were not specifically exposed to UV radiation or light except during routine handling were compared with samples which had been exposed in one of several different ways. One preparation (Fig. 2) was from a psoriasis patient who had received whole-body UVB phototherapy repeatedly.

Application of high-resolution field-emission LVSEM, backscatter imaging, immunogold staining to definition of the spatial distributions of two human neutrophil adherence receptors

1993 ◽  
Vol 51 ◽  
pp. 36-37
Author(s):  
Robert D. Nelson ◽  
Sharon R. Hasslen ◽  
Stanley L. Erlandsen
Keyword(s):  
Cell Surface ◽  
Surface Membrane ◽  
Three Dimensional ◽  
Human Neutrophils ◽  
Spatial Distributions ◽  
Immunogold Staining ◽  
Functional Control ◽  
Neutrophil Adherence ◽  
Definition Of ◽  
Mode Of Attachment

Receptors are commonly defined in terms of number per cell, affinity for ligand, chemical structure, mode of attachment to the cell surface, and mechanism of signal transduction. We propose to show that knowledge of spatial distribution of receptors on the cell surface can provide additional clues to their function and components of functional control.L-selectin and Mac-1 denote two receptor populations on the neutrophil surface that mediate neutrophil-endothelial cell adherence interactions and provide for targeting of neutrophil recruitment to sites of inflammation. We have studied the spatial distributions of these receptors using LVSEM and backscatter imaging of isolated human neutrophils stained with mouse anti-receptor (primary) antibody and goat anti-mouse (secondary) antibody conjugated to 12 nm colloidal gold. This combination of techniques provides for three-dimensional analysis of the expression of these receptors on different surface membrane domains of the neutrophil: the ruffles and microvilli that project from the cell surface, and the cell body between these projecting structures.

Direct Measurement of the Listening of Hearing-Impaired Children

1970 ◽  
Vol 13 (1) ◽  
pp. 65-73 ◽  
Author(s):  
Mary Mira
Keyword(s):  
Direct Measurement ◽  
Continuous Measurement ◽  
Hearing Impaired ◽  
Reinforcement System ◽  
School Aged Children ◽  
Conjugate Reinforcement ◽  
Impaired Children ◽  
Hearing Impaired Children

Listening, a significant dimension of the behavior of hearing-impaired children, may be measured directly by recording childrens' responses to obtain audio narrations programmed via a conjugate reinforcement system. Twelve hearing-impaired, school-aged children responded in varying ways to the opportunity to listen. Direct and continuous measurement of listening has relevance for evaluation of remediation methods and for discovery of variables potentially related to listening.

Inhibition of Apoptosis in Human Neutrophils by Helicobacter pylori Water-Soluble Surface Proteins

2001 ◽  
Vol 36 (6) ◽  
pp. 589-600 ◽  
Author(s):  
J. S. Kim ◽  
J. M. Kim ◽  
H. C. Jung ◽  
I. S. Song ◽  
C. Y. Kim
Keyword(s):  
Helicobacter Pylori ◽  
Water Soluble ◽  
Surface Proteins ◽  
Human Neutrophils

Author response for "T cell‐derived cytokines enhance the antigen‐presenting capacity of human neutrophils"

2019 ◽  
Author(s):  
Nazanin Samadi ◽  
Dominika Polak ◽  
Claudia Kitzmüller ◽  
Peter Steinberger ◽  
Gerhard J. Zlabinger ◽  
...  
Keyword(s):  
T Cell ◽  
Author Response ◽  
Human Neutrophils ◽  
Antigen Presenting
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