Real-Time Monitoring of Morphological Changes in Living Cells by Electronic Cell Sensor Arrays:  An Approach To Study G Protein-Coupled Receptors

2006 ◽  
Vol 78 (1) ◽  
pp. 35-43 ◽  
Author(s):  
Naichen Yu ◽  
Josephine M. Atienza ◽  
Jerome Bernard ◽  
Sebastien Blanc ◽  
Jenny Zhu ◽  
...  
Keyword(s):  
Real Time ◽  
G Protein ◽  
Morphological Changes ◽  
Sensor Arrays ◽  
Living Cells ◽  
G Protein Coupled Receptors ◽  
Real Time Monitoring ◽  
G Protein Coupled

scholarly journals Detecting ligand interactions with G protein-coupled receptors in real-time on living cells

2013 ◽  
Vol 441 (4) ◽  
pp. 820-824 ◽  
Author(s):  
Bo Xu ◽  
Zohreh Varasteh ◽  
Anna Orlova ◽  
Karl Andersson ◽  
Dan Larhammar ◽  
...  
Keyword(s):  
Real Time ◽  
G Protein ◽  
Living Cells ◽  
G Protein Coupled Receptors ◽  
Ligand Interactions ◽  
G Protein Coupled

Applying label-free dynamic mass redistribution technology to frame signaling of G protein–coupled receptors noninvasively in living cells

2011 ◽  
Vol 6 (11) ◽  
pp. 1748-1760 ◽  
Author(s):  
Ralf Schröder ◽  
Johannes Schmidt ◽  
Stefanie Blättermann ◽  
Lucas Peters ◽  
Nicole Janssen ◽  
...  
Keyword(s):  
G Protein ◽  
Living Cells ◽  
G Protein Coupled Receptors ◽  
Label Free ◽  
Dynamic Mass ◽  
Mass Redistribution ◽  
G Protein Coupled ◽  
Free Dynamic

scholarly journals Imaging of persistent cAMP signaling by internalized G protein-coupled receptors

2010 ◽  
Vol 45 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Davide Calebiro ◽  
Viacheslav O Nikolaev ◽  
Martin J Lohse
Keyword(s):  
G Protein ◽  
Intracellular Signaling ◽  
Current Model ◽  
Living Cells ◽  
G Protein Coupled Receptors ◽  
Membrane Receptors ◽  
Optical Methods ◽  
Camp Signaling ◽  
Gpcr Signaling ◽  
G Protein Coupled

G protein-coupled receptors (GPCRs) are the largest family of plasma membrane receptors. They mediate the effects of several endogenous cues and serve as important pharmacological targets. Although many biochemical events involved in GPCR signaling have been characterized in great detail, little is known about their spatiotemporal dynamics in living cells. The recent advent of optical methods based on fluorescent resonance energy transfer allows, for the first time, to directly monitor GPCR signaling in living cells. Utilizing these methods, it has been recently possible to show that the receptors for two protein/peptide hormones, the TSH and the parathyroid hormone, continue signaling to cAMP after their internalization into endosomes. This type of intracellular signaling is persistent and apparently triggers specific cellular outcomes. Here, we review these recent data and explain the optical methods used for such studies. Based on these findings, we propose a revision of the current model of the GPCR–cAMP signaling pathway to accommodate receptor signaling at endosomes.

scholarly journals Solubilization and Display of G Protein-Coupled Receptors on Beads for Real-Time Fluorescence and Flow Cytometric Analysis

BioTechniques ◽  
2000 ◽  
Vol 28 (5) ◽  
pp. 976-985 ◽  
Author(s):  
L.A. Sklar ◽  
J. Vilven ◽  
E. Lynam ◽  
D. Neldon ◽  
T.A. Bennett ◽  
...  
Keyword(s):  
Real Time ◽  
G Protein ◽  
Flow Cytometric Analysis ◽  
G Protein Coupled Receptors ◽  
Flow Cytometric ◽  
G Protein Coupled

scholarly journals Recruitment of Activated G Protein-coupled Receptors to Pre-existing Clathrin-coated Pits in Living Cells

2001 ◽  
Vol 277 (5) ◽  
pp. 3552-3559 ◽  
Author(s):  
Mark G. H. Scott ◽  
Alexandre Benmerah ◽  
Olivier Muntaner ◽  
Stefano Marullo
Keyword(s):  
G Protein ◽  
Living Cells ◽  
G Protein Coupled Receptors ◽  
Coated Pits ◽  
G Protein Coupled
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