Absolute quantitation of high abundant Fc-glycopeptides from human serum IgG-1

2020 ◽  
Vol 1102 ◽  
pp. 130-139 ◽  
Author(s):  
Cuiyan Cao ◽  
Long Yu ◽  
Dongmei Fu ◽  
Jingli Yuan ◽  
Xinmiao Liang
Keyword(s):  
Human Serum ◽  
Absolute Quantitation ◽  
Serum Igg

Structural Analysis of an N-Glycan with “β1−4 Bisecting Branch” from Human Serum IgG by Negative-Ion MSnSpectral Matching and Exoglycosidase Digestion

2005 ◽  
Vol 77 (18) ◽  
pp. 6062-6068 ◽  
Author(s):  
Yasuhiro Takegawa ◽  
Kisaburo Deguchi ◽  
Hiroaki Nakagawa ◽  
Shin-Ichiro Nishimura
Keyword(s):  
Structural Analysis ◽  
Human Serum ◽  
Negative Ion ◽  
Serum Igg

Development of a novel multiplex electrochemiluminescent-based immunoassay for quantification of human serum IgG against 10 Staphylococcus aureus toxins

2016 ◽  
Vol 430 ◽  
pp. 33-42 ◽  
Author(s):  
Rajan P. Adhikari ◽  
Christian Haudenschild ◽  
Patricia M. Sterba ◽  
Sara Sahandi ◽  
Sven Enterlein ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Human Serum ◽  
Serum Igg

Quantitation of Serum IgG by Solid-Phase Radioimmunoassay

1974 ◽  
Vol 20 (3) ◽  
pp. 376-382 ◽  
Author(s):  
Leo P Cawley ◽  
William L Goodwin ◽  
Marilyn Moeder ◽  
Paulette Dibbern
Keyword(s):  
Human Serum ◽  
Sensitivity And Specificity ◽  
Solid Phase ◽  
Diffusion Method ◽  
Human Igg ◽  
Serum Igg ◽  
Hospital Patients ◽  
Solid Phase Radioimmunoassay ◽  
Set Up ◽  
Gel Diffusion

Abstract Human serum immunoglobulins are most frequently measured by single gel diffusion. Radioimmunoassay by use of antibodies bonded to particles is faster and more sensitive, precise, accurate, and specific. We adopted a solid-phase radioimmunoassay (SPRIA) technique, developed in our laboratory, to measure serum IgG, and compared the results with those of a gel-diffusion method commonly used to measure serum IgG. The SPRIA technique is based on competition set up between antibodies to human IgG chemically bonded to cellulose particles and a purified serum IgG labeled with 125I. The solid phase permits prompt separation of the bound from free labeled reagent. We studied precision, accuracy, sensitivity, and specificity, as compared to gel diffusion; values for the two methods agreed well for 57 sera from hospital patients. The SPRIA procedure is more precise, and can be completed in 3 h, as compared to the 16-24 h normally required for gel diffusion procedures. The possible automation of SPRIA is discussed.

Maleylation of bovine colostral IgG and human serum IgG

1972 ◽  
Vol 9 (2) ◽  
pp. 137-IN2 ◽  
Author(s):  
Anatoly Bezkorovainy ◽  
Dietmar Grohlich
Keyword(s):  
Human Serum ◽  
Serum Igg
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