Alternating Current Impedance Imaging of Membrane Pores Using Scanning Electrochemical Microscopy

2005 ◽  
Vol 77 (17) ◽  
pp. 5564-5569 ◽  
Author(s):  
Eric Nathan Ervin ◽  
Henry S. White ◽  
Lane A. Baker
Keyword(s):  
Scanning Electrochemical Microscopy ◽  
Alternating Current ◽  
Membrane Pores ◽  
Impedance Imaging ◽  
Electrochemical Microscopy

The Alternating Current Scanning Electrochemical Microscopy Characterization of Diatom Cell Activity Affected by Paeonol

2014 ◽  
Vol 707 ◽  
pp. 113-116 ◽  
Author(s):  
Shou Huan Zhao ◽  
Ji Yong Zheng ◽  
Jing Ya Yang ◽  
Cun Guo Lin ◽  
Wei Wang ◽  
...  
Keyword(s):  
Cell Activity ◽  
Scanning Electrochemical Microscopy ◽  
Alternating Current ◽  
Electrochemical Activity ◽  
Chemical Stimulus ◽  
Cellular Activity ◽  
High Frequencies ◽  
Microscopy Characterization ◽  
Electrochemical Microscopy

The alternating current scanning electrochemical microscopy (AC-SECM) is an important method to characterize the electrochemical activity of living cells. In this paper, we apply AC-SECM to measure the electrochemical activity of diatom biofilm. We find that the diatom biofilm presents insulating effect to decrease the current, when the electrode is close to the biofilm. The alternating current impedance of diatom biofilm is overall smaller than that of the bare glass. To investigate the possible effect of chemical stimulus on cellular activity, the diatoms were immersed into paeonol solution (100 μg/mL), and AC-SECM imaging experiments were conducted when immersion time reached 20 h, 25 h and 35 h. The AC measurements performed at high frequencies show that phase angle decreases over time, which preliminarily indicates that the cellular activity of diatoms is affected by paeonol.

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