Electrochemically Mediated Reduction of Horseradish Peroxidase by 1,1‘-Ferrocenedimethanol in Organic Solvents

2005 ◽  
Vol 77 (6) ◽  
pp. 1647-1654 ◽  
Author(s):  
Anastassija Konash ◽  
Edmond Magner
Keyword(s):  
Horseradish Peroxidase ◽  
Organic Solvents ◽  
Mediated Reduction

scholarly journals Decoloration Reaction of Orange II Catalyzed by Horseradish Peroxidase in Organic Solvents/water System

2005 ◽  
Vol 54 (11) ◽  
pp. 577-583 ◽  
Author(s):  
Manami MORISAKI ◽  
Miyuki MORITA ◽  
Kiyoshi ITO
Keyword(s):  
Horseradish Peroxidase ◽  
Organic Solvents ◽  
Water System ◽  
Orange Ii

Electrochemistry of Immobilized Horseradish Peroxidase in Organic Solvents/Water Mixture

2006 ◽  
Vol 22 (07) ◽  
pp. 868-872
Author(s):  
LIU Hui-Hong ◽  
◽  
◽  
WAN Yong-Qing ◽  
CHEN Xian-Tang ◽  
...  
Keyword(s):  
Horseradish Peroxidase ◽  
Organic Solvents ◽  
Water Mixture

scholarly journals Gene mining, codon optimization and analysis of binding mechanism of an aldo-keto reductase with high activity, better substrate specificity and excellent solvent tolerance

PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0260787
Author(s):  
Wei Jiang ◽  
Xiaoli Fu ◽  
Weiliang Wu
Keyword(s):  
High Activity ◽  
Organic Solvents ◽  
Catalytic Reduction ◽  
Antidepressant Drug ◽  
Reaction System ◽  
Solvent Tolerance ◽  
Chiral Alcohols ◽  
Binding Mechanism ◽  
Potential Applications ◽  
Mediated Reduction

The biosynthesis of chiral alcohols has important value and high attention. Aldo–keto reductases (AKRs) mediated reduction of prochiral carbonyl compounds is an interesting way of synthesizing single enantiomers of chiral alcohols due to the high enantio-, chemo- and regioselectivity of the enzymes. However, relatively little research has been done on characterization and apply of AKRs to asymmetric synthesis of chiral alcohols. In this study, the AKR from Candida tropicalis MYA-3404 (C. tropicalis MYA-3404), was mined and characterized. The AKR shown wider optimum temperature and pH. The AKR exhibited varying degrees of catalytic activity for different substrates, suggesting that the AKR can catalyze a variety of substrates. It is worth mentioning that the AKR could catalytic reduction of keto compounds with benzene rings, such as cetophenone and phenoxyacetone. The AKR exhibited activity on N,N-dimethyl-3-keto-3-(2-thienyl)-1-propanamine (DKTP), a key intermediate for biosynthesis of the antidepressant drug duloxetine. Besides, the AKR still has high activity whether in a reaction system containing 10%-30% V/V organic solvent. What’s more, the AKR showed the strongest stability in six common organic solvents, DMSO, acetonitrile, ethyl acetate, isopropanol, ethanol, and methanol. And, it retains more that 70% enzyme activity after 6 hours, suggesting that the AKR has strong solvent tolerance. Furthermore, the protein sequences of the AKR and its homology were compared, and a 3D model of the AKR docking with coenzyme NADPH were constructed. And the important catalytic and binding sites were identified to explore the binding mechanism of the enzyme and its coenzyme. These properties, predominant organic solvents resistance and extensive substrate spectrum, of the AKR making it has potential applications in the pharmaceutical field.

scholarly journals Design and Characterization of a Novel Artificial Peroxidase

Catalysts ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 168 ◽  
Author(s):  
Ye Yuan ◽  
Jia Xu ◽  
Zhenyu Zhao ◽  
Hui Li ◽  
Kai Wang ◽  
...  
Keyword(s):  
Horseradish Peroxidase ◽  
Kinetic Parameters ◽  
Organic Solvents ◽  
New Method ◽  
Quantitative Detection ◽  
Ping Pong ◽  
Excellent Stability

In this study, we reported on a novel mimetic peroxidase, deuterohemin–Ala–His–Glu, (Dh–A–H–E). The kinetic parameters of Dh–A–H–E suggested that it was a mimetic peroxidase and followed the ping–pong mechanism. Compared to horseradish peroxidase, Dh–A–H–E exhibited excellent stability when tested at different pH and temperatures, and using different organic solvents. Based on our above results, a new method using Dh–A–H–E has successfully been developed for the fast and quantitative detection of trace amounts of glucose.

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