Tandem Mass Tags:  A Novel Quantification Strategy for Comparative Analysis of Complex Protein Mixtures by MS/MS

2003 ◽  
Vol 75 (8) ◽  
pp. 1895-1904 ◽  
Author(s):  
Andrew Thompson ◽  
Jürgen Schäfer ◽  
Karsten Kuhn ◽  
Stefan Kienle ◽  
Josef Schwarz ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Tandem Mass ◽  
Complex Protein ◽  
Tandem Mass Tags

scholarly journals Tandem Mass Tags:  A Novel Quantification Strategy for Comparative Analysis of Complex Protein Mixtures by MS/MS

2006 ◽  
Vol 78 (12) ◽  
pp. 4235-4235 ◽  
Author(s):  
Andrew Thompson ◽  
Juergen Schaefer ◽  
Karsten Kuhn ◽  
Stefan Kienle ◽  
Josef Schwarz ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Tandem Mass ◽  
Complex Protein ◽  
Tandem Mass Tags

scholarly journals Tandem Mass Tags:  A Novel Quantification Strategy for Comparative Analysis of Complex Protein Mixtures by MS/MS

2003 ◽  
Vol 75 (18) ◽  
pp. 4942-4942 ◽  
Author(s):  
Andrew Thompson ◽  
Jürgen Schäfer ◽  
Karsten Kuhn ◽  
Stefan Kienle ◽  
Josef Schwarz ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Tandem Mass ◽  
Complex Protein ◽  
Tandem Mass Tags

Exploration of the effect of salvianolate on myocardial infarction in rats based on tandem mass tags

2020 ◽  
Vol 889 ◽  
pp. 173610
Author(s):  
Cheng Chen ◽  
Pengli Zhu ◽  
Huizhen Yu ◽  
Bin Huang ◽  
Mengxuan Gui ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Tandem Mass ◽  
Tandem Mass Tags

scholarly journals Quantitative Proteomics Analysis of Altered Protein Expression in the Placental Villous Tissue of Early Pregnancy Loss Using Isobaric Tandem Mass Tags

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Xiaobei Ni ◽  
Xin Li ◽  
Yueshuai Guo ◽  
Tao Zhou ◽  
Xuejiang Guo ◽  
...  
Keyword(s):  
Protein Expression ◽  
Pregnant Women ◽  
Early Pregnancy ◽  
Proteomic Analysis ◽  
Pregnancy Loss ◽  
Tandem Mass ◽  
Pathophysiological Mechanism ◽  
Early Pregnancy Loss ◽  
Expression Levels ◽  
Tandem Mass Tags

Many pregnant women suffer miscarriages during early gestation, but the description of these early pregnancy losses (EPL) can be somewhat confusing because of the complexities of early development. Thus, the identification of proteins with different expression profiles related to early pregnancy loss is essential for understanding the comprehensive pathophysiological mechanism. In this study, we report a gel-free tandem mass tags- (TMT-) labeling based proteomic analysis of five placental villous tissues from patients with early pregnancy loss and five from normal pregnant women. The application of this method resulted in the identification of 3423 proteins and 19647 peptides among the patient group and the matched normal control group. Qualitative and quantitative proteomic analysis revealed 51 proteins to be differentially abundant between the two groups (≥1.2-fold, Student'st-test,P<0.05). To obtain an overview of the biological functions of the proteins whose expression levels altered significantly in EPL group, gene ontology analysis was performed. We also investigated the twelve proteins with a difference over 1.5-fold using pathways analysis. Our results demonstrate that the gel-free TMT-based proteomic approach allows the quantification of differences in protein expression levels, which is useful for obtaining molecular insights into early pregnancy loss.

scholarly journals Accurate and Sensitive Quantitation of the Dynamic Heat Shock Proteome Using Tandem Mass Tags

2020 ◽  
Vol 19 (3) ◽  
pp. 1183-1195 ◽  
Author(s):  
Aaron J. Storey ◽  
Rebecca E. Hardman ◽  
Stephanie D. Byrum ◽  
Samuel G. Mackintosh ◽  
Rick D. Edmondson ◽  
...  
Keyword(s):  
Heat Shock ◽  
Tandem Mass ◽  
Tandem Mass Tags

scholarly journals Evosep One Enables Robust Deep Proteome Coverage Using Tandem Mass Tags while Significantly Reducing Instrument Time

2019 ◽  
Vol 18 (5) ◽  
pp. 2346-2353 ◽  
Author(s):  
Jonathan R. Krieger ◽  
Leanne E. Wybenga-Groot ◽  
Jiefei Tong ◽  
Nicolai Bache ◽  
Ming S. Tsao ◽  
...  
Keyword(s):  
Tandem Mass ◽  
Proteome Coverage ◽  
Tandem Mass Tags
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