Chemicallyl-Phenylalaninamide-Modified Monolithic Silica Column Prepared by a Sol−Gel Process for Enantioseparation of Dansyl Amino Acids by Ligand Exchange-Capillary Electrochromatography

2001 ◽  
Vol 73 (14) ◽  
pp. 3348-3357 ◽  
Author(s):  
Zilin Chen ◽  
Toshiyuki Hobo
Keyword(s):  
Amino Acids ◽  
Ligand Exchange ◽  
Capillary Electrochromatography ◽  
Sol Gel ◽  
Dansyl Amino Acids ◽  
Monolithic Silica ◽  
Silica Column ◽  
Sol Gel Process ◽  
Monolithic Silica Column

scholarly journals Polydopamine-Assisted Rapid One-Step Immobilization of L-Arginine in Capillary as Immobilized Chiral Ligands for Enantioseparation of Dansyl Amino Acids by Chiral Ligand Exchange Capillary Electrochromatography

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1800
Author(s):  
Yuanqi Gui ◽  
Baian Ji ◽  
Gaoyi Yi ◽  
Xiuju Li ◽  
Kailian Zhang ◽  
...  
Keyword(s):  
Amino Acids ◽  
Ligand Exchange ◽  
Capillary Electrochromatography ◽  
Metal Ion ◽  
High Performance ◽  
Basic Amino Acid ◽  
Chiral Ligand ◽  
Central Metal ◽  
Dansyl Amino Acids ◽  
Chiral Ligand Exchange

Herein, a novel L-arginine (L-Arg)-modified polydopamine (PDA)-coated capillary (PDA/L-Arg@capillary) was firstly fabricated via the basic amino-acid-induced PDA co-deposition strategy and employed to constitute a new chiral ligand exchange capillary electrochromatography (CLE-CEC) method for the high-performance enantioseparation of D,L-amino acids (D,L-AAs) with L-Arg as the immobilized chiral ligand coordinating with the central metal ion Zn(II) as running buffer. Assisted by hydrothermal treatment, the robust immobilization of L-Arg on the capillary inner wall could be facilely achieved within 1 h, prominently improving the synthesis efficiency and simplifying the preparation procedure. The successful preparation of PDA/L-Arg coatings in the capillary was systematically characterized and confirmed using several methods. In comparison with bare and PDA-functionalized capillaries, the enantioseparation capability of the presented CLE-CEC system was significantly enhanced. Eight D,L-AAs were completely separated and three pairs were partially separated under the optimal conditions. The prepared PDA/L-Arg@capillary showed good repeatability and stability. The potential mechanism of the greatly enhanced enantioseparation performance obtained by PDA/L-Arg@capillary was also explored. Moreover, the proposed method was further utilized for studying the enzyme kinetics of L-glutamic dehydrogenase, exhibiting its promising prospects in enzyme assays and other related applications.

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