Minimization of errors in fixed-time reaction rate methods by optimization of measurement time

1982 ◽  
Vol 54 (4) ◽  
pp. 755-761 ◽  
Author(s):  
F. J. Holler ◽  
R. K. Calhoun ◽  
S. F. McClanahan
1962 ◽  
Vol 8 (6) ◽  
pp. 606-615 ◽  
Author(s):  
H V Malmstadt ◽  
H L Pardue

Abstract A new automatic potentiometric reaction-rate method has been applied to the specific enzymatic measurement of glucose in blood plasma or serum. A new filtering technic is described for removal of precipitated protein. The use of injection pipets to simplify and speed up the reagent-and sample-handling step is described. Glucose is determined in 0.02 ml. of serum or plasma with relative errors within 2%. The average measurement time is about 30 sec.


1979 ◽  
Vol 25 (4) ◽  
pp. 611-613 ◽  
Author(s):  
P K Li ◽  
B C Shull

Abstract We describe a fixed-time, enzymatic, reaction-rate procedure for determining plasma ammonia with a centrifugal analyzer (Rotochem IIA/36; American Instrument Co., silver Spring, MD 20910), with NADPH as cofactor. The reaction is based on that of da Fonseca-Wollheim's modification [J. Clin. Chem. Clin. Biochem. 11, 421 (1973)] of the Kirstein reaction, which depends on the catalytic amination of alpha-ketoglutarate by the action of glutamate dehydrogenase with NADPH as the cofactor instead of NADH. Use of NADPH minimizes interference from endogenous reactions such as that between lactate dehydrogenase and pyruvate. This method permits shortened preincubation time and thus improves both specificity and precision. This assay requires 100 microliter of freshly collected heparinized plasma, gives quantitative analytical recovery, and the standard curve is linear to 430 mumol/L. Data are presented comparing results with those by two other enzymatic ammonia procedures.


1980 ◽  
Vol 26 (12) ◽  
pp. 1713-1717 ◽  
Author(s):  
P K Li ◽  
J T Lee ◽  
M H MacGillivray ◽  
P A Schaefer ◽  
J H Siegel

Abstract In the course of studying the control of blood glucose in juvenile onset diabetics, we developed convenient methods for determining beta-hydroxybutyrate and acetoacetate. Here we describe fixed-time, enzymic, reaction-rate procedures for directly measuring these organic acids with a centrifugal analyzer (Rotochem IIA/36) or a computer-backed spectrophotometer (Gilford 102 system). In either case, the method requires only 20 micro L of plasma; is rapid, accurate, and precise; and analytical recovery is quantitative. Data are presented comparing results obtained with both instruments. Metabolic acidosis can be rapidly assessed and monitored with these methods, as illustrated by an example.


1970 ◽  
Vol 42 (9) ◽  
pp. 1055-1060 ◽  
Author(s):  
James D. Ingle ◽  
Stanley R. Crouch

Author(s):  
Kathleen B. Reuter

The reaction rate and efficiency of piperazine to 1,4-diazabicyclo-octane (DABCO) depends on the Si/Al ratio of the MFI topology catalysts. The Al was shown to be the active site, however, in the Si/Al range of 30-200 the reaction rate increases as the Si/Al ratio increases. The objective of this work was to determine the location and concentration of Al to explain this inverse relationship of Al content with reaction rate.Two silicalite catalysts in the form of 1/16 inch SiO2/Al2O3 bonded extrudates were examined: catalyst A with a Si/Al of 83; and catalyst B, the acid/phosphate Al extracted form of catalyst A, with a Si/Al of 175. Five extrudates from each catalyst were fractured in the transverse direction and particles were obtained from the fracture surfaces near the center of the extrudate diameter. Particles were also obtained from the outside surfaces of five extrudates.


Author(s):  
M. Sato ◽  
Y. Ogawa ◽  
M. Sasaki ◽  
T. Matsuo

A virgin female of the noctuid moth, a kind of noctuidae that eats cucumis, etc. performs calling at a fixed time of each day, depending on the length of a day. The photoreceptors that induce this calling are located around the neurosecretory cells (NSC) in the central portion of the protocerebrum. Besides, it is considered that the female’s biological clock is located also in the cerebral lobe. In order to elucidate the calling and the function of the biological clock, it is necessary to clarify the basic structure of the brain. The observation results of 12 or 30 day-old noctuid moths showed that their brains are basically composed of an outer and an inner portion-neural lamella (about 2.5 μm) of collagen fibril and perineurium cells. Furthermore, nerve cells surround the cerebral lobes, in which NSCs, mushroom bodies, and central nerve cells, etc. are observed. The NSCs are large-sized (20 to 30 μm dia.) cells, which are located in the pons intercerebralis of the head section and at the rear of the mushroom body (two each on the right and left). Furthermore, the cells were classified into two types: one having many free ribosoms 15 to 20 nm in dia. and the other having granules 150 to 350 nm in dia. (Fig. 1).


Author(s):  
C. S. Lin ◽  
W. A. Chiou ◽  
M. Meshii

The galvannealed steel sheets have received ever increased attention because of their excellent post-painting corrosion resistance and good weldability. However, its powdering and flaking tendency during press forming processes strongly impairs its performance. In order to optimize the properties of galvanneal coatings, it is critical to control the reaction rate between solid iron and molten zinc.In commercial galvannealing line, aluminum is added to zinc bath to retard the diffusion rate between iron and zinc by the formation of a thin layer of Al intermetallic compound on the surface of steel at initial hot-dip galvanizing. However, the form of this compound and its transformation are still speculated. In this paper, we report the direct observations of this compound and its transformation.The specimens were prepared in a hot-dip simulator in which the steel was galvanized in the zinc bath containing 0.14 wt% of Al at a temperature of 480 °C for 5 seconds and was quenched by liquid nitrogen.


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