Master the basics of this powerful analytical tool

1989 ◽  
Vol 61 (6) ◽  
pp. 440A-440A
2012 ◽  
Vol 24 (3) ◽  
pp. 213-223 ◽  
Author(s):  
Rosa Quirantes-Piné ◽  
Jesús Lozano-Sánchez ◽  
Miguel Herrero ◽  
Elena Ibáñez ◽  
Antonio Segura-Carretero ◽  
...  

2020 ◽  
Vol 21 (20) ◽  
pp. 7465 ◽  
Author(s):  
Vasilisa V. Krasitskaya ◽  
Eugenia E. Bashmakova ◽  
Ludmila A. Frank

The functioning of bioluminescent systems in most of the known marine organisms is based on the oxidation reaction of the same substrate—coelenterazine (CTZ), catalyzed by luciferase. Despite the diversity in structures and the functioning mechanisms, these enzymes can be united into a common group called CTZ-dependent luciferases. Among these, there are two sharply different types of the system organization—Ca2+-regulated photoproteins and luciferases themselves that function in accordance with the classical enzyme–substrate kinetics. Along with deep and comprehensive fundamental research on these systems, approaches and methods of their practical use as highly sensitive reporters in analytics have been developed. The research aiming at the creation of artificial luciferases and synthetic CTZ analogues with new unique properties has led to the development of new experimental analytical methods based on them. The commercial availability of many ready-to-use assay systems based on CTZ-dependent luciferases is also important when choosing them by first-time-users. The development of analytical methods based on these bioluminescent systems is currently booming. The bioluminescent systems under consideration were successfully applied in various biological research areas, which confirms them to be a powerful analytical tool. In this review, we consider the main directions, results, and achievements in research involving these luciferases.


1980 ◽  
Vol 1 (2) ◽  
pp. 229-249 ◽  
Author(s):  
A. H. W. M. Schuurs ◽  
B. K. Van Weemen

1972 ◽  
Vol 24 (4) ◽  
pp. 518-546 ◽  
Author(s):  
James L. Payne ◽  
Oliver H. Woshinsky

Why are people in politics? Behind this deceptively simple question lies a promising realm of research and analysis. A satisfactory understanding of political motivation can provide a powerful analytical tool for explaining why different groups of participants behave as they do; ultimately it can suggest why they adopt and sustain different political institutions. The basic axiom which, in principle, yields such explanations is simple: participants behave in a manner consistent with their incentives.


Author(s):  
Wolfgang Kandioller ◽  
Johannes Theiner ◽  
Bernhard K. Keppler ◽  
Christian R. Kowol

Elemental analysis is a powerful analytical tool for purity determination. However, it is prone to manipulations. Herein, we introduce an approach to provide original elemental analysis data, allowing verification of the currently just listed values.


2011 ◽  
Vol 20 (3) ◽  
Author(s):  
Alan I. Blankley ◽  
Philip G. Cottell Jr. ◽  
Richard H. McClure

<p class="MsoBodyText" style="text-align: justify; margin: 0in 0.5in 0pt;"><span style="font-size: 10pt; mso-bidi-font-style: italic;"><span style="font-family: Times New Roman;">Because future pension expense can have a material influence on a firm&rsquo;s future earnings, financial analysts are faced with the difficult task of forecasting its impact.<span style="mso-spacerun: yes;">&nbsp; </span>The purpose of this paper is to demonstrate a model that can be used with a simulation approach to predict future pension expense and its associated uncertainties.<span style="mso-spacerun: yes;">&nbsp; </span>Because of the importance and complexity of the pension expense component in the estimate of future earnings, a simulation model acts as a powerful analytical tool that can give the analyst greater confidence as to the magnitude and variability of future pension expense.</span></span></p>


Author(s):  
D. J. H. Cockayne

The appearance of lattice fringe images formed from perfect crystals is sensitive to parameters such as lens defocus, beam orientation and foil thickness. For certain values of these parameters the lattice fringe image shows contrast which can be interpreted directly in terms of the projection of the crystal structure. Extensive computations and image simulation have established the conditions under which this direct interpretation is valid, and the method is now recognized as a powerful analytical tool at the limit of resolution of the electron microscope.


Author(s):  
Jong-Shing Bow ◽  
Wen-Tung Chang ◽  
Yun-Ming Tsou ◽  
Hsing-Shuang Chou ◽  
Christ Chiou

Abstract A 300 keV TEM equipped with an EELS and energy selected imaging (ESI) system has proven to be a necessary and powerful analytical tool for R&D and failure analysis support. The advantages and the efficacy of this advanced elemental mapping technique can be optimized when the TEM is operated correctly. Variants of ESI, elemental mapping, jump-ratio mapping, and pre-edge imaging have been adapted to explore root causes of different type of failures in semiconductor manufacturing.


1985 ◽  
Vol 5 (10) ◽  
pp. 2842-2846
Author(s):  
M A Lopata ◽  
B Sollner-Webb ◽  
D W Cleveland

Although the technique of S1 mapping is a powerful analytical tool for the analysis of RNA, we now report a surprising complication involving a trimolecular hybrid between two RNA species and a single DNA probe molecule which, if unrecognized, can lead to misleading interpretations. We document that such trimolecular hybrids can be efficiently formed under some hybridization conditions and that the probe DNA sequence at the junction of the two RNA molecules can be remarkably stable to digestion with S1. Trimolecular hybrids can arise in any instance whenever a distal region of an end-labeled DNA probe is homologous to a moderately abundant RNA in the sample to be analyzed. This situation presents a serious, potential complication for a variety of S1 analyses, particularly those in which DNA transfection has been utilized to reintroduce in vitro-engineered genes into cultured animal cells.


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