Determination of sodium and potassium in nanoliter volumes of biological fluids by furnace atomic absorption spectrometry

1988 ◽  
Vol 60 (21) ◽  
pp. 2413-2418 ◽  
Author(s):  
Lori. Nash ◽  
Linda N. Peterson ◽  
Steven P. Nadler ◽  
David Z. Levine
Keyword(s):  
Atomic Absorption Spectrometry ◽  
Atomic Absorption ◽  
Biological Fluids ◽  
Absorption Spectrometry ◽  
Sodium And Potassium

Indirect measurement of desferrioxamine and its chelated compounds aluminoxamine and ferrioxamine by Zeeman atomic absorption spectrometry.

1989 ◽  
Vol 35 (5) ◽  
pp. 884-887 ◽  
Author(s):  
P C D'Haese ◽  
L V Lamberts ◽  
M E De Broe
Keyword(s):  
Atomic Absorption Spectrometry ◽  
Atomic Absorption ◽  
Biological Fluids ◽  
Metal Chelate ◽  
Absorption Spectrometry ◽  
Indirect Measurement ◽  
Calibration Technique ◽  
Iron Aluminum ◽  
Chelate Formation

Abstract This indirect method for determination of desferrioxamine (DFO, deferoxamine, Desferal) and its chelated compounds ferrioxamine (FO) and aluminoxamine (AIO) in biological fluids involves metal chelate formation and solvent extraction, the metal being determined by Zeeman atomic absorption spectrometry (Zeeman-AAS). Special attention was paid to the pH and recovery of extraction, iron-aluminum interactions, extraction times and volumes, centrifugation rates, and instrument settings. Precautions were taken to avoid contamination with aluminum and iron and to prevent co-extraction of iron and aluminum not chelated to DFO. The addition calibration technique was used for standardization. Mean +/- SD recoveries of AIO and FO after extraction were respectively 98.3% +/- 3.7% and 101% +/- 3.7%. Sensitivity was 45 pg/0.0044 A.s for aluminum and 10 pg/0.0044 A.s for iron. Detection limits were 0.22 mumol/L for AIO, 0.06 mumol/L for DFO and FO.

Sign in / Sign up

Export Citation Format

Share Document