Two-Layer Sample Preparation Method for MALDI Mass Spectrometric Analysis of Protein and Peptide Samples Containing Sodium Dodecyl Sulfate

2001 ◽  
Vol 73 (13) ◽  
pp. 2968-2975 ◽  
Author(s):  
Nan Zhang ◽  
Alan Doucette ◽  
Liang Li
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Sample Preparation ◽  
Preparation Method ◽  
Sodium Dodecyl ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis ◽  
Sample Preparation Method ◽  
Dodecyl Sulfate ◽  
Layer Sample

scholarly journals Simple Sodium Dodecyl Sulfate-Assisted Sample Preparation Method for LC-MS-Based Proteomics Applications

2012 ◽  
Vol 84 (6) ◽  
pp. 2862-2867 ◽  
Author(s):  
Jian-Ying Zhou ◽  
Geoffrey P. Dann ◽  
Tujin Shi ◽  
Lu Wang ◽  
Xiaoli Gao ◽  
...  
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Sample Preparation ◽  
Preparation Method ◽  
Sodium Dodecyl ◽  
Sample Preparation Method ◽  
Dodecyl Sulfate

scholarly journals Removal of sodium dodecyl sulfate from protein samples

Chemija ◽  
2018 ◽  
Vol 29 (4) ◽  
Author(s):  
Andrius Žilionis
Keyword(s):  
Liquid Chromatography ◽  
Sodium Dodecyl Sulfate ◽  
Protein Denaturation ◽  
Sodium Dodecyl ◽  
Reversed Phase ◽  
Spectrometric Analysis ◽  
Photometric Method ◽  
Ionization Mass ◽  
Dodecyl Sulfate ◽  
Model Protein

Sodium dodecyl sulfate (SDS) is a widely used detergent for protein denaturation and solubilization. However, application of SDS in the sample preparation for the liquid chromatographic-mass spectrometric analysis is limited because commonly used SDS concentrations interfere with reversed phase liquid chromatography and electrospray ionization mass spectrometry. In order to analyse SDS pretreated proteins by the above-mentioned methods SDS must be completely removed or its concentration must be lowered to less than 0.01%. In this work we present a comparison of different SDS removal strategies based on SDS ultrafiltration, protein precipitation and SDS precipitation methods. Every strategy was optimized so that the initial 4% SDS concentration was lowered to less than 0.01% and the initial sample volume remained unchanged. The modified Mukerjee’s photometric method was used for the SDS quantitation in the presence of model protein bovine serum albumin and the recovery of model protein was evaluated using reversed phase ultra performance liquid chromatography. The main advantages and drawbacks of every strategy are discussed.

Suppression of sodium dodecyl sulfate–polyacrylamide gel electrophoresis sample preparation artifacts for analysis of IgG4 half-antibody

2006 ◽  
Vol 353 (2) ◽  
pp. 204-208 ◽  
Author(s):  
Frederick R. Taylor ◽  
Holly L. Prentice ◽  
Ellen A. Garber ◽  
Hans A. Fajardo ◽  
Elena Vasilyeva ◽  
...  
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Sample Preparation ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Polyacrylamide Gel ◽  
Dodecyl Sulfate
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