Recovery of Sodium Dodecyl Sulfate–Proteins from Gel Electrophoretic Bands in a Single Electroelution Step for Mass Spectrometric Analysis

2000 ◽  
Vol 284 (2) ◽  
pp. 288-295 ◽  
Author(s):  
Stanislav Yefimov ◽  
Christin Sjomeling ◽  
Alfred L. Yergey ◽  
Tao Li ◽  
Andreas Chrambach
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Sodium Dodecyl ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis ◽  
Dodecyl Sulfate

scholarly journals Removal of sodium dodecyl sulfate from protein samples

Chemija ◽  
2018 ◽  
Vol 29 (4) ◽  
Author(s):  
Andrius Žilionis
Keyword(s):  
Liquid Chromatography ◽  
Sodium Dodecyl Sulfate ◽  
Protein Denaturation ◽  
Sodium Dodecyl ◽  
Reversed Phase ◽  
Spectrometric Analysis ◽  
Photometric Method ◽  
Ionization Mass ◽  
Dodecyl Sulfate ◽  
Model Protein

Sodium dodecyl sulfate (SDS) is a widely used detergent for protein denaturation and solubilization. However, application of SDS in the sample preparation for the liquid chromatographic-mass spectrometric analysis is limited because commonly used SDS concentrations interfere with reversed phase liquid chromatography and electrospray ionization mass spectrometry. In order to analyse SDS pretreated proteins by the above-mentioned methods SDS must be completely removed or its concentration must be lowered to less than 0.01%. In this work we present a comparison of different SDS removal strategies based on SDS ultrafiltration, protein precipitation and SDS precipitation methods. Every strategy was optimized so that the initial 4% SDS concentration was lowered to less than 0.01% and the initial sample volume remained unchanged. The modified Mukerjee’s photometric method was used for the SDS quantitation in the presence of model protein bovine serum albumin and the recovery of model protein was evaluated using reversed phase ultra performance liquid chromatography. The main advantages and drawbacks of every strategy are discussed.

Improved mass spectrometric identification of gel-separated hydrophobic membrane proteins after sodium dodecyl sulfate removal by ion-pair extraction

PROTEOMICS ◽  
2004 ◽  
Vol 4 (12) ◽  
pp. 3776-3782 ◽  
Author(s):  
Hans Zischka ◽  
Christian Johannes Gloeckner ◽  
Christian Klein ◽  
Stefanie Willmann ◽  
Magdalena Swiatek-de Lange ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Sodium Dodecyl Sulfate ◽  
Sodium Dodecyl ◽  
Ion Pair ◽  
Mass Spectrometric ◽  
Hydrophobic Membrane ◽  
Sulfate Removal ◽  
Spectrometric Identification ◽  
Dodecyl Sulfate ◽  
Mass Spectrometric Identification

A Questionnaire Survey on General Status and Opinions about Clinical Mass Spectrometric Analysis in Korea (2018)

2019 ◽  
Vol 9 (3) ◽  
pp. 161
Author(s):  
Sung-Eun Cho ◽  
Hyojin Chae ◽  
Hyung-Doo Park ◽  
Sail Chun ◽  
Yong-Wha Lee ◽  
...  
Keyword(s):  
Questionnaire Survey ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis ◽  
General Status

Mechanisms of Dopant Depth Profile Modification During Mass Spectrometric Analysis of Multilayer Structure

2015 ◽  
Vol 60 (6) ◽  
pp. 511-520 ◽  
Author(s):  
A.A. Efremov ◽  
◽  
V.G. Litovchenko ◽  
V.P. Melnik ◽  
O.S. Oberemok ◽  
...  
Keyword(s):  
Depth Profile ◽  
Multilayer Structure ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis ◽  
Profile Modification

Mass Spectrometric Analysis of Porcine Rhodopsin¶

2002 ◽  
Vol 75 (3) ◽  
pp. 316 ◽  
Author(s):  
Zsolt Ablonczy ◽  
Patrice Goletz ◽  
Daniel R. Knapp ◽  
Rosalie K. Crouch
Keyword(s):  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis
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