Elimination of High-Voltage Field Effects in End Column Electrochemical Detection in Capillary Electrophoresis by Use of On-Chip Microband Electrodes

2001 ◽  
Vol 73 (8) ◽  
pp. 1909-1915 ◽  
Author(s):  
Oliver Klett ◽  
Fredrik Björefors ◽  
Leif Nyholm
Keyword(s):  
Capillary Electrophoresis ◽  
Electrochemical Detection ◽  
High Voltage ◽  
Field Effects ◽  
On Chip

A Capillary Electrophoresis Platform With “On-Chip” Electrochemical Detection: Experimental and Computational Flow Studies

2001 ◽  
Author(s):  
Thomas J. Roussel ◽  
Robert S. Keynton ◽  
Kevin M. Walsh ◽  
Mark M. Crain ◽  
John F. Naber ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Electrochemical Detection ◽  
Power Supply ◽  
Electroosmotic Flow ◽  
Plug Flow ◽  
Finite Element Models ◽  
Separation Channel ◽  
Flow Models ◽  
On Chip ◽  
Electrochemical Potentials

Abstract The purpose of this study was to compare experimental electrokinetic plug flow velocities to computational flow models of microfabricated capillaries. Electroosmotic flow studies of dichlorofluorescein and electrophoretic separation of dopamine and catechol in a microfabricated capillary electrophoresis (CE) system were performed both experimentally and computationally. A “balanced cross design” consisting of a bent 2 cm long injection channel and a straight 2 cm long separation channel was used. The geometry of the capillary was 65 μm wide and 20 μm deep. For the fluorescein study, separation voltages ranging between 0.25 kV and 1 kV were applied, while voltages ranging from 100 V to 550 V were used in the separation studies. Laser Induced Fluorescent (LIF) images were obtained for flow visualization and qualitative analysis in the electroosmotic flow studies, while electrochemical potentials were acquired using “on-chip” electrodes interfaced to a custom-designed power supply and electrochemical detection (ECD) circuit. Finite element models of the experimental device were generated and flows were simulated using commercially available software. For the electroosmotic flow studies, the computational results were found to be within ± 11% of the experimentally obtained values. Similarly, the results of the computational separations of catechol and dopamine predicted plug velocities that were within ± 7.6% of the experimentally determined values.

Fully Integrated On-Chip Electrochemical Detection for Capillary Electrophoresis in a Microfabricated Device

2002 ◽  
Vol 74 (15) ◽  
pp. 3690-3697 ◽  
Author(s):  
Richard P. Baldwin ◽  
Thomas J. Roussel, ◽  
Mark M. Crain ◽  
Vijay Bathlagunda ◽  
Douglas J. Jackson ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Electrochemical Detection ◽  
Fully Integrated ◽  
On Chip

Portable High-Voltage Power Supply and Electrochemical Detection Circuits for Microchip Capillary Electrophoresis

2003 ◽  
Vol 75 (14) ◽  
pp. 3643-3649 ◽  
Author(s):  
Douglas J. Jackson ◽  
John F. Naber ◽  
Thomas J. Roussel, ◽  
Mark M. Crain ◽  
Kevin M. Walsh ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Electrochemical Detection ◽  
High Voltage ◽  
Power Supply ◽  
Microchip Capillary Electrophoresis ◽  
High Voltage Power Supply

Application of Diamond Microelectrodes for End-Column Electrochemical Detection in Capillary Electrophoresis

2003 ◽  
Vol 75 (3) ◽  
pp. 530-534 ◽  
Author(s):  
Dongchan Shin ◽  
Bulusu V. Sarada ◽  
Donald A. Tryk ◽  
Akira Fujishima ◽  
Joseph Wang
Keyword(s):  
Capillary Electrophoresis ◽  
Electrochemical Detection
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