Direct Analysis of Affinity-Bound Analytes by MALDI/TOF MS

1994 ◽  
Vol 66 (17) ◽  
pp. 2609-2613 ◽  
Author(s):  
Damon I. Papac ◽  
John. Hoyes ◽  
Kenneth B. Tomer
Keyword(s):  
Direct Analysis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Rapid Pathogen Identification With Direct Application of MALDI-TOF Mass Spectrometry on an Endophthalmitis Vitreous Sample Without Prior Culture

2019 ◽  
Vol 3 (4) ◽  
pp. 255-259 ◽  
Author(s):  
Lindsay Y. Chun ◽  
Laura Dolle Molle ◽  
Olaf Schneewind ◽  
Dominique Missiakas ◽  
Kathleen G. Beavis ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Direct Analysis ◽  
Causative Organism ◽  
Pathogen Identification ◽  
Maldi Tof Ms ◽  
Hand Motion ◽  
Maldi Tof ◽  
Microbiological Methods ◽  
Vitreous Sample ◽  
Tof Ms

Purpose:We report a case of a 72-year-old man with bleb-related endophthalmitis (BRE) whose vitreous samples were directly analyzed with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to rapidly identify the causative organism, whereas the results from conventional microbiological techniques were negative.Methods:We analyzed BRE vitreous samples with MALDI-TOF MS (Vitek MS, bioMérieux) for rapid pathogen identification without prior culture. Samples were also analyzed with standard microbiological methods.Results:Within 1 hour of sample acquisition, MALDI-TOF MS identified Gemella sanguinis from the undiluted vitreous sample from vitrectomy without prior culture with a confidence value of 99.7%. Gram stain and cultures from aqueous and vitreous samples were negative for 28 days after acquisition. The patient’s right-eye vision improved from hand motion to 20/50 2 months later.Conclusions:Our findings suggest that the direct analysis of intraocular samples with MALDI-TOF MS could be a novel, promising adjuvant method of rapid endophthalmitis diagnosis.

Characterization of Phosphopeptides Using a Combination of Immobilized Metal Ion Affinity Media and Direct Analysis by MALDI-TOF-MS

2007 ◽  
Vol 2007 (8) ◽  
pp. pdb.prot4618-pdb.prot4618
Author(s):  
L. J. Deterding ◽  
J. M. Cutalo ◽  
K. B. Tomer
Keyword(s):  
Metal Ion ◽  
Direct Analysis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Direct analysis of positive blood cultures using MALDI-TOF MS profiling and a fundamentally improved sample preparation technique

Pathology ◽  
2011 ◽  
Vol 43 ◽  
pp. S73-S74
Author(s):  
Guido Mix ◽  
Leith Fremlin ◽  
Thomas Maier ◽  
Beatrix Wegemann ◽  
Markus Kostrzewa
Keyword(s):  
Sample Preparation ◽  
Direct Analysis ◽  
Blood Cultures ◽  
Preparation Technique ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Sample Preparation Technique ◽  
Tof Ms

scholarly journals Application of MALDI-TOF MS Profiling Coupled With Functionalized Magnetic Enrichment for Rapid Identification of Pathogens in a Patient With Open Fracture

2021 ◽  
Vol 9 ◽  
Author(s):  
Jichong Ying ◽  
Wenjing Gao ◽  
Dichao Huang ◽  
Chuanfan Ding ◽  
Ling Ling ◽  
...  
Keyword(s):  
Direct Analysis ◽  
Opportunistic Pathogen ◽  
Antimicrobial Treatment ◽  
Clinical Samples ◽  
Trauma Patients ◽  
Orthopedic Trauma ◽  
Accurate Identification ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Posttraumatic infections can occur in orthopedic trauma patients, especially in open fractures. Rapid and accurate identification of pathogens in orthopedic trauma is important for clinical diagnosis and antimicrobial treatment. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been successfully used for first-line identification of pathogens grown on culture plates. However, for direct analysis of liquid clinical specimens, pre-purification of the sample is necessary. Herein, we investigated the feasibility of coupling Fc-MBL@Fe3O4 enrichment with MALDI-TOF MS profiling in the identification of pathogens in liquid-cultured samples. This method is successfully used for the identification of pathogens in a patient with an open-leg fracture obtained at sea. Pathogens were enriched by Fc-MBL@Fe3O4 from briefly pre-cultured liquid media and identified by MALDI-TOF MS. We identified an opportunistic pathogen, Vibrio alginolyticus, which is uncommon in clinical orthopedic trauma infection but exists widely in the sea. Therefore, combining Fc-MBL@Fe3O4 enrichment and MALDI-TOF MS profiling has great potential for direct identification of microbes in clinical samples.

Photoacid generator formation for the selective enrichment of perfluoroalkyl sulfonates and their direct analysis by MALDI-TOF-MS

The Analyst ◽  
2011 ◽  
Vol 136 (11) ◽  
pp. 2225 ◽  
Author(s):  
Dong Cao ◽  
Ming Hu ◽  
Chunguang Han ◽  
Jiyao Yu ◽  
Lin Cui ◽  
...  
Keyword(s):  
Direct Analysis ◽  
Maldi Tof Ms ◽  
Selective Enrichment ◽  
Maldi Tof ◽  
Photoacid Generator ◽  
Tof Ms

scholarly journals An Economical High-Throughput Protocol for Multidimensional Fractionation of Proteins

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
David John Tooth ◽  
Varun Gopala Krishna ◽  
Robert Layfield
Keyword(s):  
High Throughput ◽  
Mobile Phase ◽  
Gel Permeation Chromatography ◽  
Direct Analysis ◽  
Reversed Phase ◽  
Cytoskeletal Proteins ◽  
Cell Protein ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

A sequential protocol of multidimensional fractionation was optimised to enable the comparative profiling of fractions of proteomes from cultured human cells. Differential detergent fractionation was employed as a first step to obtain fractions enriched for cytosolic, membrane/organelle, nuclear, and cytoskeletal proteins. Following buffer exchange using gel-permeation chromatography, cytosolic proteins were further fractionated by 2-dimensional chromatography employing anion-exchange followed by reversed-phase steps. Chromatographic fractions were shown to be readily compatible with 1- and 2-dimensional gel electrophoresis or with direct analysis by mass spectrometry using linear-MALDI-TOF-MS. Precision of extraction was confirmed by reproducible SDS-PAGE profiles, MALDI-TOF-MS spectra, and quantitation of trypsinolytic peptides using LC-MS/MS (MRM) analyses. Solid phases were immobilised in disposable cartridges and mobile-phase flow was achieved using a combination of centrifugation and vacuum pumping. These approaches yielded parallel sample handling which was limited only by the capacities of the employed devices and which enabled both high-throughput and experimentally precise procedures, as demonstrated by the processing of experimental replicates. Protocols were employed at 10 mg scale of extracted cell protein, but these approaches would be directly applicable to both smaller and larger quantities merely by adjusting the employed solid- and mobile-phase volumes. Additional potential applications of the fractionation protocol are briefly described.

scholarly journals Coupled TLC and MALDI-TOF/MS Analyses of the Lipid Extract of the Hyperthermophilic ArchaeonPyrococcus furiosus

Archaea ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Simona Lobasso ◽  
Patrizia Lopalco ◽  
Roberto Angelini ◽  
Rita Vitale ◽  
Harald Huber ◽  
...  
Keyword(s):  
Pyrococcus Furiosus ◽  
Ether Lipid ◽  
Direct Analysis ◽  
Lipid Extract ◽  
Total Lipid Extract ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Chromatography Plate ◽  
Layer Chromatography ◽  
Tof Ms

The lipidome of the marine hyperthermophilic archaeonPyrococcus furiosuswas studied by means of combined thin-layer chromatography and MALDI-TOF/MS analyses of the total lipid extract. 80–90% of the major polar lipids were represented by archaeol lipids (diethers) and the remaining part by caldarchaeol lipids (tetraethers). The direct analysis of lipids on chromatography plate showed the presence of the diphytanylglycerol analogues of phosphatidylinositol and phosphatidylglycerol, theN-acetylglucosamine-diphytanylglycerol phosphate plus some caldarchaeol lipids different from those previously described. In addition, evidence for the presence of the dimeric ether lipid cardiolipin is reported, suggesting that cardiolipins are ubiquitous in archaea.

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