Analysis of Protein Phosphorylation by a Combination of Elastase Digestion and Neutral Loss Tandem Mass Spectrometry

2001 ◽  
Vol 73 (2) ◽  
pp. 170-176 ◽  
Author(s):  
Andreas Schlosser ◽  
Ruediger Pipkorn ◽  
Dirk Bossemeyer ◽  
Wolf D. Lehmann
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Protein Phosphorylation ◽  
Neutral Loss ◽  
Tandem Mass

scholarly journals Analysis of non-enzymatically glycated peptides: neutral-loss-triggered MS3versus multi-stage activation tandem mass spectrometry

2008 ◽  
Vol 22 (19) ◽  
pp. 3027-3034 ◽  
Author(s):  
Qibin Zhang ◽  
Vladislav A. Petyuk ◽  
Athena A. Schepmoes ◽  
Daniel J. Orton ◽  
Matthew E. Monroe ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Neutral Loss ◽  
Tandem Mass ◽  
Multi Stage

scholarly journals METLIN Neutral Loss Database Enhances Similarity Analysis

2021 ◽  
Author(s):  
Gary Siuzdak ◽  
Aries Aisporna ◽  
H. Paul Benton ◽  
Jean-Marie Galano ◽  
Martin Giera
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Neutral Loss ◽  
Structural Similarity ◽  
Similarity Analysis ◽  
Tandem Mass ◽  
Loss Data

Tandem mass spectrometry (MS2) data is an effective resource for the identification of known molecules and the putative identification of novel, previously uncharacterized molecules (unknowns). Yet, MS2 data alone is limited in characterizing structurally closely related molecules with different masses. Neutral loss data is key in retrieving this structural similarity. To facilitate unknown identification and complement METLIN fragment ion data for characterizing structurally related molecules, we have created the METLIN neutral loss database (https://metlin-nl.scripps.edu).

scholarly journals Discovery of Unexpected Sphingolipids in Almonds and Pistachios with an Innovative Use of Triple Quadrupole Tandem Mass Spectrometry

Foods ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 110 ◽  
Author(s):  
Federico Maria Rubino ◽  
Michele Dei Cas ◽  
Monica Bignotto ◽  
Riccardo Ghidoni ◽  
Marcello Iriti ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Neutral Loss ◽  
Spectrometric Method ◽  
Total Lipid Extract ◽  
Tandem Mass ◽  
Mass Spectrometric Method ◽  
Triple Quadrupole ◽  
Loss Experiment ◽  
Long Chain

The densely packed storage of valuable nutrients (carbohydrates, lipids, proteins, micronutrients) in the endosperm of nuts and seeds makes the study of their complex composition a topic of great importance. Ceramides in the total lipid extract of some ground almonds and pistachios were searched with a systematic innovative discovery precursor ion scan in a triple quadrupole tandem mass spectrometry, where iso-energetic collision activated dissociation was performed. Five descriptors were used to search components with different C18 long chain bases containing different structural motifs (d18:0, d18:1, d18:2, t18:0, t18:1). The presence of hexoside unit was screened with a specific neutral loss experiment under iso-energetic collision activated dissociation conditions. The discovery scans highlighted the presence of two specific hexosyl-ceramides with a modified sphingosine component (d18:2) and C16:0 or C16:0 hydroxy-fatty acids. The hexosyl-ceramide with the non-hydroxylated fatty acid seemed specific of pistachios and was undetected in almonds. The fast and comprehensive mass spectrometric method used here can be useful to screen lipid extracts of several more seeds of nutraceutical interest, searching for unusual and/or specific sphingosides with chemically decorated long chain bases.

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