Analysis of fluorescence lifetime data for single Rhodamine molecules in flowing sample streams

1994 ◽  
Vol 66 (1) ◽  
pp. 64-72 ◽  
Author(s):  
Joel. Tellinghuisen ◽  
Peter M. Goodwin ◽  
W. Patrick. Ambrose ◽  
John C. Martin ◽  
Richard A. Keller
Keyword(s):  
Fluorescence Lifetime ◽  
Lifetime Data ◽  
Flowing Sample

scholarly journals A parameter estimation method for fluorescence lifetime data

2015 ◽  
Vol 8 (1) ◽  
Author(s):  
Daniel Sewell ◽  
Hajin Kim ◽  
Taekjip Ha ◽  
Ping Ma
Keyword(s):  
Parameter Estimation ◽  
Fluorescence Lifetime ◽  
Estimation Method ◽  
Lifetime Data ◽  
Parameter Estimation Method

scholarly journals A biosensor of local kinesin activity reveals roles of PKC and EB1 in KIF17 activation

2013 ◽  
Vol 203 (3) ◽  
pp. 445-455 ◽  
Author(s):  
Cedric Espenel ◽  
Bipul R. Acharya ◽  
Geri Kreitzer
Keyword(s):  
Energy Transfer ◽  
Fluorescence Lifetime ◽  
Single Cells ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Epithelial Differentiation ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Data ◽  
Lifetime Imaging ◽  
Phasor Plot

We showed previously that the kinesin-2 motor KIF17 regulates microtubule (MT) dynamics and organization to promote epithelial differentiation. How KIF17 activity is regulated during this process remains unclear. Several kinesins, including KIF17, adopt compact and extended conformations that reflect autoinhibited and active states, respectively. We designed biosensors of KIF17 to monitor its activity directly in single cells using fluorescence lifetime imaging to detect Förster resonance energy transfer. Lifetime data are mapped on a phasor plot, allowing us to resolve populations of active and inactive motors in individual cells. Using this biosensor, we demonstrate that PKC contributes to the activation of KIF17 and that this is required for KIF17 to stabilize MTs in epithelia. Furthermore, we show that EB1 recruits KIF17 to dynamic MTs, enabling its accumulation at MT ends and thus promoting MT stabilization at discrete cellular domains.

Characterization of crude oils using fluorescence lifetime data

2002 ◽  
Vol 58 (5) ◽  
pp. 1025-1037 ◽  
Author(s):  
A.G Ryder ◽  
T.J Glynn ◽  
M Feely ◽  
A.J.G Barwise
Keyword(s):  
Fluorescence Lifetime ◽  
Crude Oils ◽  
Lifetime Data

scholarly journals Study of Cellular Uptake of Modified Oligonucleotides by Using Time-Resolved Microspectrofluorimetry and Florescence Imaging

2012 ◽  
Vol 27 ◽  
pp. 415-419 ◽  
Author(s):  
P. Praus ◽  
E. Kočišová ◽  
P. Mojzeš ◽  
J. Štěpánek ◽  
F. Sureau
Keyword(s):  
Cell Line ◽  
Cellular Uptake ◽  
Fluorescence Lifetime ◽  
Antisense Oligonucleotide ◽  
Malignant Cell ◽  
Lifetime Data ◽  
Modified Oligonucleotides ◽  
Mcf7 Cells ◽  
Time Resolved ◽  
Modified Oligonucleotide

Fluorescence microimaging and homodyne phase-resolved confocal microspectrofluorimetry were used to monitor the transport of antisense oligonucleotide into cancer MCF7 cells and the subsequent intracellular distribution. Phosphorothioate analog of 15-mer oligoadenylate (dA15) labeled by ATTO 425 was complexed with 5,10,15,20-tetrakis (1-methyl-4-pyridyl) porphyrin (H2TMPyP4) as an uptake-mediating agent. Fluorescence lifetime data within a broad spectral range have revealed properties of both components inside the cell. H2TMPyP4 lifetime inside the cell is not influenced in this malignant cell line, while the lifetime of modified oligonucleotide was found to be slightly shortened.

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