Comparison of the maximum entropy and exponential series methods for the recovery of distributions of lifetimes from fluorescence lifetime data

1990 ◽  
Vol 94 (4) ◽  
pp. 1661-1666 ◽  
Author(s):  
Aleksander. Siemiarczuk ◽  
Brian D. Wagner ◽  
William R. Ware
Keyword(s):  
Maximum Entropy ◽  
Fluorescence Lifetime ◽  
Lifetime Data ◽  
Exponential Series

scholarly journals A parameter estimation method for fluorescence lifetime data

2015 ◽  
Vol 8 (1) ◽  
Author(s):  
Daniel Sewell ◽  
Hajin Kim ◽  
Taekjip Ha ◽  
Ping Ma
Keyword(s):  
Parameter Estimation ◽  
Fluorescence Lifetime ◽  
Estimation Method ◽  
Lifetime Data ◽  
Parameter Estimation Method

scholarly journals A biosensor of local kinesin activity reveals roles of PKC and EB1 in KIF17 activation

2013 ◽  
Vol 203 (3) ◽  
pp. 445-455 ◽  
Author(s):  
Cedric Espenel ◽  
Bipul R. Acharya ◽  
Geri Kreitzer
Keyword(s):  
Energy Transfer ◽  
Fluorescence Lifetime ◽  
Single Cells ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Epithelial Differentiation ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Data ◽  
Lifetime Imaging ◽  
Phasor Plot

We showed previously that the kinesin-2 motor KIF17 regulates microtubule (MT) dynamics and organization to promote epithelial differentiation. How KIF17 activity is regulated during this process remains unclear. Several kinesins, including KIF17, adopt compact and extended conformations that reflect autoinhibited and active states, respectively. We designed biosensors of KIF17 to monitor its activity directly in single cells using fluorescence lifetime imaging to detect Förster resonance energy transfer. Lifetime data are mapped on a phasor plot, allowing us to resolve populations of active and inactive motors in individual cells. Using this biosensor, we demonstrate that PKC contributes to the activation of KIF17 and that this is required for KIF17 to stabilize MTs in epithelia. Furthermore, we show that EB1 recruits KIF17 to dynamic MTs, enabling its accumulation at MT ends and thus promoting MT stabilization at discrete cellular domains.

Characterization of crude oils using fluorescence lifetime data

2002 ◽  
Vol 58 (5) ◽  
pp. 1025-1037 ◽  
Author(s):  
A.G Ryder ◽  
T.J Glynn ◽  
M Feely ◽  
A.J.G Barwise
Keyword(s):  
Fluorescence Lifetime ◽  
Crude Oils ◽  
Lifetime Data
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