Microenvironments of fluorescence probes in sodium taurocholate and sodium taurodeoxycholate bile salt media

1991 ◽  
Vol 63 (19) ◽  
pp. 2082-2086 ◽  
Author(s):  
Steven M. Meyerhoffer ◽  
Linda B. McGown
Keyword(s):  
Bile Salt ◽  
Sodium Taurocholate ◽  
Fluorescence Probes ◽  
Sodium Taurodeoxycholate

Behaviors of Sodium Taurocholate and Sodium Taurodeoxycholate in Binary Mixed Micelles of Bile Salt and Nonionic Surfactant

Langmuir ◽  
1996 ◽  
Vol 12 (23) ◽  
pp. 5536-5540 ◽  
Author(s):  
K. Suzuki ◽  
T. Hasegawa ◽  
Y. Takamura ◽  
K. Takahashi ◽  
H. Asano ◽  
...  
Keyword(s):  
Bile Salt ◽  
Nonionic Surfactant ◽  
Mixed Micelles ◽  
Sodium Taurocholate ◽  
Sodium Taurodeoxycholate

scholarly journals Enzymic hydrolysis of sphingomyelin in the presence of bile salts

1980 ◽  
Vol 185 (3) ◽  
pp. 749-754 ◽  
Author(s):  
S Yedgar ◽  
S Gatt
Keyword(s):  
Bile Salt ◽  
Bile Salts ◽  
Sodium Taurocholate ◽  
Absolute Concentration ◽  
Mixed Aggregates ◽  
Sodium Taurodeoxycholate ◽  
Rate Of Hydrolysis ◽  
The Absolute ◽  
Hydrolysis Of ◽  
Low Activity

Sphingomyelin in mixed dispersion with bile salts was hydrolysed by the solubilized sphingomyelinase of rat brain lysosomes. In parallel studies, physical properties of these dispersions were determined. The kinetic curves that described the rate of hydrolysis as a function of increasing concentrations of bile salt were multiphasic. A region of very low activity was followed by an ascending portion, a peak, a descending portion, a trough and a second ascending portion. The positions of the initiation points, peaks and troughs were found to be a function of the respective ratios of the bile salt to sphingomyelin for the detergent sodium taurodeoxycholate, but of the absolute concentration of the detergent for sodium taurocholate. Turbidity studies suggested that hydrolysis of sphingomyelin begins at a bile salt concentration that solubilizes the lipid and incorporates it into a mixed micelle with the detergent. Ultracentrifugation studies suggested that the sizes of the mixed aggregates of detergent and lipid were a function of the ratio of taurodeoxycholate to sphingomyelin, but of the absolute concentration of the bile salt, for sodium taurocholate.

Protein kinase C induces endocytosis of the sodium taurocholate cotransporting polypeptide

2010 ◽  
Vol 299 (2) ◽  
pp. G320-G328 ◽  
Author(s):  
Claudia Stross ◽  
Angelika Helmer ◽  
Katrin Weissenberger ◽  
Boris Görg ◽  
Verena Keitel ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Bile Salt ◽  
Fluorescent Protein ◽  
Membrane Surface ◽  
Sodium Taurocholate ◽  
Surface Expression ◽  
Cell Surface Expression ◽  
Salt Uptake ◽  
Kinase C

Bile salts influence signaling and metabolic pathways. In hepatocytes, the sodium taurocholate cotransporting polypeptide (Ntcp) is a major determinant of intracellular bile salt levels. Short-term downregulation of Ntcp is not well characterized to date. FLAG and enhanced green fluorescent protein (EGFP) tags were cloned to the extra- and intracellular termini of Ntcp. Endocytosis of Ntcp in transfected HepG2 cells was visualized by fluorescence of EGFP, and membrane surface expression of Ntcp was quantified by flow cytometry with fluorochrome-labeled FLAG antibodies. Activation of protein kinase C (PKC) by phorbolester or thymeleatoxin an activator of Ca2+-dependent conventional PKCs (cPKCs), induced endocytosis of Ntcp, whereas the Na+-K+-ATPase remained in the plasma membrane. The PKC inhibitor BIM I and the cPKC-selective inhibitor Gö6976 abolished PMA-induced endocytosis. Because of this internalization, cell surface expression of Ntcp was reduced by 36 ± 7%, bile salt uptake was decreased by 25%, and taurolithocholate sulfate-induced cell toxicity was prevented. In conclusion, Ca2+-dependent PKCs induce vesicular retrieval of Ntcp, thereby reducing bile salt uptake. This mechanism may protect hepatocytes from toxic intracellular bile salt concentrations.

Effect of intestinal resection on bile salt absorption in dogs

1965 ◽  
Vol 208 (2) ◽  
pp. 363-369 ◽  
Author(s):  
M. R. Playoust ◽  
Leon Lack ◽  
I. M. Weiner
Keyword(s):  
Bile Salt ◽  
Bile Salts ◽  
Enterohepatic Circulation ◽  
Sodium Taurocholate ◽  
Intestinal Resection ◽  
High Fat ◽  
Salt Absorption ◽  
Complete Failure ◽  
Fat Meal

The efficiency of intestinal absorption of bile salts was evaluated by studying the rate of disappearance of radioactivity from the bile of dogs after the intravenous administration of sodium taurocholate-24-C14. Bile was sampled through an indwelling tube in the gall bladder. One day after a high-fat meal normal dogs retained 48% of the radioactivity; dogs with resection of the jejunum retained 48%, whereas those with resection of the ileum retained only 3% in the bile. This is consistent with previous observations that the ileum is the site of bile salt absorption in vitro and in anesthetized animals. Animals with resection of the ileum exhibited significant steatorrhea; however, three-fourths of the ingested fat was absorbed in spite of almost complete failure to absorb bile salts. This indicates that fat and bile salts are not normally absorbed together. Elimination of enterohepatic circulation of bile salts by resection of the ileum contributes to the observed steatorrhea.

The Role of the Sodium-taurocholate Co-transporting Polypeptide (NTCP) and Bile Salt Export Pump (BSEP) in Related Liver Disease

2019 ◽  
Vol 20 (5) ◽  
pp. 377-389 ◽  
Author(s):  
Xiaoyang Lu ◽  
Lin Liu ◽  
Wenya Shan ◽  
Limin Kong ◽  
Na Chen ◽  
...  
Keyword(s):  
Liver Disease ◽  
Structure Function ◽  
Bile Salt ◽  
Drug Targets ◽  
Enterohepatic Circulation ◽  
Sodium Taurocholate ◽  
Bile Salt Export Pump ◽  
Bile Acid Transporters ◽  
Function Relationship ◽  
Related Liver Disease

Background:Sodium Taurocholate Co-transporting Polypeptide (NTCP) and Bile Salt Export Pump (BSEP) play significant roles as membrane transporters because of their presence in the enterohepatic circulation of bile salts. They have emerged as promising drug targets in related liver disease.Methods:We reviewed the literature published over the last 20 years with a focus on NTCP and BSEP.Results:This review summarizes the current perception about structure, function, genetic variation, and regulation of NTCP and BSEP, highlights the effects of their defects in some hepatic disorders, and discusses the application prospect of new transcriptional activators in liver diseases.Conclusion:NTCP and BSEP are important proteins for transportation and homeostasis maintenance of bile acids. Further research is needed to develop new models for determining the structure-function relationship of bile acid transporters and screening for substrates and inhibitors, as well as to gain more information about the regulatory genetic mechanisms involved in the processes of liver injury.

Heat Stability of the Bile Salt-Stimulated Lipase in Human Milk Fortified with Sodium Taurocholate1

1988 ◽  
Vol 51 (4) ◽  
pp. 310-313 ◽  
Author(s):  
H. L. PAN ◽  
C. W. DILL ◽  
E. S. ALFORD ◽  
S. L. DILL ◽  
C. A. BAILEY ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Human Milk ◽  
Bile Salt ◽  
Lipase Activity ◽  
Sodium Taurocholate ◽  
Heat Stability ◽  
Heat Inactivation ◽  
Temperature Differential ◽  
Heat Stable ◽  
Control Samples

Time-temperature relationships for heat-inactivation of the bile salt-stimulated lipase activity were compared in whole human milk and in the same product fortified to 9 mM/ml with sodium taurocholate. Heat treatments were varied from 45 to 70°C for times ranging from 15s to 40 min. Enzyme activity was more heat stable in human milk fortified with taurocholate than in control samples. The temperature required for the onset of heat inactivation at 30-min holding time was increased from 45°C for control samples to 60°C following addition of taurocholate. A temperature differential of approximately 12°C was required in the fortified milks to produce inactivation equivalent to that observed in the control milks over the heating range studied.

Effect of glycodihydrofuisidate on sulfobromophthalein transport maximum in the hamster

1976 ◽  
Vol 231 (6) ◽  
pp. 1875-1878 ◽  
Author(s):  
Y Delage ◽  
M Dumont ◽  
S Erlinger
Keyword(s):  
Bile Salt ◽  
Transport System ◽  
Bile Salts ◽  
Sodium Taurocholate ◽  
Specific Effect ◽  
Biliary Secretion ◽  
Biliary Lipid ◽  
Lipid Secretion ◽  
Dye Transport ◽  
Cholesterol Secretion

The effect on sulfobromophathalein transport maximum (Tm) and biliary lipid secretion of sodium glyco-24,25-dihydrofusicate, a micelle-forming compound secreted into bile, has been studied in the hamster and compared to that of a physiological bile salt, sodium taurocholate. Biliary phospholipid and cholesterol secretion increased both during glycodihydrofusidate and taurocholate administration, an observation which suggest that both compounds increased th biliary secretion of micelle-forming compounds. In contrast, only taurocholate increased sulfobromophthalein Tm into bile, while glycodihydrofusidate administration decreased it. This observation suggests that the increase in sulfobromophthalein Tm observed during taurocholate administration is not the result of micellar sequestration. It could rather be the consequence of a specific effect of bile salts on the dye transport system.

scholarly journals The Lipid Raft Component Stomatin Interacts with the Na+ Taurocholate Cotransporting Polypeptide (NTCP) and Modulates Bile Salt Uptake

Cells ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 986
Author(s):  
Monique D. Appelman ◽  
Marion J.D. Robin ◽  
Esther W.M. Vogels ◽  
Christie Wolzak ◽  
Winnie G. Vos ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Bile Salt ◽  
Protein Interactions ◽  
Basolateral Membrane ◽  
Sodium Taurocholate ◽  
Salt Transport ◽  
Protein Protein Interactions ◽  
Cell Surface Biotinylation ◽  
Detergent Resistant Membranes ◽  
Entry Receptor

The sodium taurocholate cotransporting polypeptide (NTCP) is expressed at the basolateral membrane of hepatocytes, where it mediates the uptake of conjugated bile acids and forms the hepatocyte entry receptor for the hepatitis B and D virus. Here, we aimed to identify novel protein–protein interactions that could play a role in the regulation of NTCP. To this end, NTCP was precipitated from HA-tagged hNTCP-expressing HepG2 cells, and chloride channel CLIC-like 1 (CLCC1) and stomatin were identified as interacting proteins by mass spectrometry. Interaction was confirmed by co-immunoprecipitation. NTCP, CLCC1 and stomatin were found at the plasma membrane in lipid rafts, as demonstrated by a combination of immunofluorescence, cell surface biotinylation and isolation of detergent-resistant membranes. Neither CLCC1 overexpression nor its knockdown had an effect on NTCP function. However, both stomatin overexpression and knockdown increased NTCP-mediated taurocholate uptake while NTCP abundance at the plasma membrane was only increased in stomatin depleted cells. These findings identify stomatin as an interactor of NTCP and show that the interaction modulates bile salt transport.

How does bile salt penetration affect the self-assembled architecture of pluronic P123 micelles? – light scattering and spectroscopic investigations

2015 ◽  
Vol 17 (30) ◽  
pp. 19977-19990 ◽  
Author(s):  
Arpita Roy ◽  
Niloy Kundu ◽  
Debasis Banik ◽  
Jagannath Kuchlyan ◽  
Nilmoni Sarkar
Keyword(s):  
Light Scattering ◽  
Bile Salt ◽  
Bile Salts ◽  
Triblock Copolymer ◽  
Sodium Taurocholate ◽  
Sodium Deoxycholate ◽  
The Self ◽  
Spectroscopic Investigations ◽  
Pluronic P123 ◽  
Supramolecular Aggregate

The triblock copolymer of the type (PEO)20–(PPO)70–(PEO)20 (P123) forms a mixed supramolecular aggregate with different bile salts, sodium deoxycholate (NaDC) and sodium taurocholate (NaTC), having different hydrophobicity.

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