Bladder Acellular Matrix Graft Reinforced Silk Fibroin Composite Scaffolds Loaded VEGF with Aligned Electrospun Fibers in Multiple Layers

2015 ◽  
Vol 1 (4) ◽  
pp. 238-246 ◽  
Author(s):  
Zhaobo Li ◽  
Qiangqiang Liu ◽  
Hongsheng Wang ◽  
Lujie Song ◽  
Huili Shao ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Electrospun Fibers ◽  
Composite Scaffolds ◽  
Acellular Matrix

Prevascularized bladder acellular matrix hydrogel/silk fibroin composite scaffolds promote the regeneration of urethra in a rabbit model

2018 ◽  
Vol 14 (1) ◽  
pp. 015002 ◽  
Author(s):  
Nailong Cao ◽  
Lujie Song ◽  
Wenjing Liu ◽  
Suna Fan ◽  
Dan Jiang ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Rabbit Model ◽  
Composite Scaffolds ◽  
Acellular Matrix

Tough and VEGF-releasing scaffolds composed of artificial silk fibroin mats and a natural acellular matrix

RSC Advances ◽  
2015 ◽  
Vol 5 (22) ◽  
pp. 16748-16758 ◽  
Author(s):  
Zhaobo Li ◽  
Lujie Song ◽  
Xiangyu Huang ◽  
Hongsheng Wang ◽  
Huili Shao ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Silk Fibroin ◽  
Composite Scaffolds ◽  
Cell Compatibility ◽  
Acellular Matrix ◽  
Good Cell

The blend and coaxially electrospun RSF/BAMG composite scaffolds loaded VEGF exhibited good cell compatibility with improved mechanical properties.

Characteristics and Osteoconductivity of Bone Composite Scaffolds Made of Thai Silk Fibroin, Gelatin and Inorganic Compounds: A Comparative Study of β-Tricalcium Phosphate and Hydroxyapatite

2015 ◽  
Vol 354 (1) ◽  
pp. 258-264 ◽  
Author(s):  
Chotika Dararutana ◽  
Juthamas Ratanavaraporn ◽  
Sittisak Honsawek ◽  
Sorada Kanokpanont ◽  
Siriporn Damrongsakkul
Keyword(s):  
Comparative Study ◽  
Silk Fibroin ◽  
Tricalcium Phosphate ◽  
Inorganic Compounds ◽  
Composite Scaffolds

Electrospinning of Regenerated Silk Fibroin/Polybutylene Terephthalate Blended Mats and their Surface Wettability

2012 ◽  
Vol 531-532 ◽  
pp. 531-534
Author(s):  
Yun Qian Cao ◽  
Qin Fei Ke ◽  
Xiang Yu Jin ◽  
Sha Sha Guo
Keyword(s):  
Silk Fibroin ◽  
Fiber Diameter ◽  
Contact Angles ◽  
Surface Wettability ◽  
Electrospun Fibers ◽  
Average Fiber Diameter ◽  
Polybutylene Terephthalate ◽  
Regenerated Silk Fibroin ◽  
Electrospinning Method ◽  
Nanofiber Membranes

In this paper, regenerated silk fibroin/polybutylene terephthalate blended mats were prepared using electrospinning method with different blending ratios. The influence of regenerated silk fibroin/polybutylene terephthalate ratio on the morphology behaviors, fiber diameter and the surface wettability of the blended mats were studied. The morphology of the electrospun fibers were characterized by SEM. The average fiber diameter and its distribution can be obtained from the SEM pictures using software Image J. The average fiber diameter was 280nm to 486nm and it changed with the blending ratio. The contact angles and penetration times were used to characterize the surface wettability of the nanofiber membranes. It was found that with the increase of regenerated silk fibroin amount, the surface contact angles and penetration times decreased, which meant that the wettability was greatly improved.

scholarly journals The Fabrication and Evaluation of a Potential Biomaterial Produced with Stem Cell Sheet Technology for Future Regenerative Medicine

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Shukui Zhou ◽  
Ying Wang ◽  
Kaile Zhang ◽  
Nailong Cao ◽  
Ranxing Yang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Epithelial Cells ◽  
Regenerative Medicine ◽  
Silk Fibroin ◽  
Surgical Complication ◽  
Cell Sheet ◽  
The Body ◽  
Acellular Matrix ◽  
Triton X

To date, the decellularized scaffold has been widely explored as a source of biological scaffolds for regenerative medicine. However, the acellular matrix derived from natural tissues and organs has a lot of defects, including the limited amount of autogenous tissue and surgical complication such as risk of blood loss, wound infection, pain, shock, and functional damage in the donor part of the body. In this study, we prepared acellular matrix using adipose-derived stem cell (ADSC) sheets and evaluate the cellular compatibility and immunoreactivity. The ADSC sheets were fabricated and subsequently decellularized using repeated freeze-thaw, Triton X-100 and SDS decellularization. Oral mucosal epithelial cells were seeded onto the decellularized ADSC sheets to evaluate the cell replantation ability, and silk fibroin was used as the control. Then, acellular matrix was transplanted onto subcutaneous tissue for 1 week or 3 weeks; H&E staining and immunohistochemical analysis of CD68 expression and quantitative real-time PCR (qPCR) were performed to evaluate the immunogenicity and biocompatibility. The ADSC sheet-derived ECM scaffolds preserved the three-dimensional architecture of ECM and retained the cytokines by Triton X-100 decellularization protocols. Compared with silk fibroin in vitro, the oral mucosal epithelial cells survived better on the decellularized ADSC sheets with an intact and consecutive epidermal cellular layer. Compared with porcine small intestinal submucosa (SIS) in vivo, the homogeneous decellularized ADSC sheets had less monocyte-macrophage infiltrating in vivo implantation. During 3 weeks after transplantation, the mRNA expression of cytokines, such as IL-4/IL-10, was obviously higher in decellularized ADSC sheets than that of porcine SIS. A Triton X-100 method can achieve effective cell removal, retain major ECM components, and preserve the ultrastructure of ADSC sheets. The decellularized ADSC sheets possess good recellularization capacity and excellent biocompatibility. This study demonstrated the potential suitability of utilizing acellular matrix from ADSC sheets for soft tissue regeneration and repair.

Silk fibroin tissue engineering scaffolds with aligned electrospun fibers in multiple layers

RSC Advances ◽  
2014 ◽  
Vol 4 (88) ◽  
pp. 47570-47575 ◽  
Author(s):  
Nannan Jiang ◽  
Xiangyu Huang ◽  
Zhaobo Li ◽  
Lujie Song ◽  
Hongsheng Wang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Silk Fibroin ◽  
Electrospun Fibers ◽  
Tissue Engineering Scaffolds

Preparation, characterization and biocompatible properties of β-chitin/silk fibroin/nanohydroxyapatite composite scaffolds prepared using a freeze-drying method

RSC Advances ◽  
2016 ◽  
Vol 6 (9) ◽  
pp. 7048-7060 ◽  
Author(s):  
Mohammad Azadi ◽  
Abbas Teimouri ◽  
Ghasem Mehranzadeh
Keyword(s):  
Silk Fibroin ◽  
Freeze Drying ◽  
Drying Method ◽  
Composite Scaffolds ◽  
Organic Weight

β-Chitin/silk fibroin/nanohydroxyapatite (CT/SF/nHAp) composite scaffolds were synthesized using a freeze-drying method by blending β-chitin hydrogel, silk fibroin and nHAp at different inorganic/organic weight ratios.

scholarly journals Synergistic Effects on Incorporation of β-Tricalcium Phosphate and Graphene Oxide Nanoparticles to Silk Fibroin/Soy Protein Isolate Scaffolds for Bone Tissue Engineering

Polymers ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 69 ◽  
Author(s):  
Fan Liu ◽  
Chen Liu ◽  
Bowen Zheng ◽  
Jia He ◽  
Jun Liu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Silk Fibroin ◽  
Soy Protein ◽  
Tricalcium Phosphate ◽  
Soy Protein Isolate ◽  
Protein Isolate ◽  
Composite Scaffolds

In bone tissue engineering, an ideal scaffold is required to have favorable physical, chemical (or physicochemical), and biological (or biochemical) properties to promote osteogenesis. Although silk fibroin (SF) and/or soy protein isolate (SPI) scaffolds have been widely used as an alternative to autologous and heterologous bone grafts, the poor mechanical property and insufficient osteoinductive capability has become an obstacle for their in vivo applications. Herein, β-tricalcium phosphate (β-TCP) and graphene oxide (GO) nanoparticles are incorporated into SF/SPI scaffolds simultaneously or individually. Physical and chemical properties of these composite scaffolds are evaluated using field emission scanning electron microscope (FESEM), X-ray diffraction (XRD) and attenuated total reflectance Fourier transformed infrared spectroscopy (ATR-FTIR). Biocompatibility and osteogenesis of the composite scaffolds are evaluated using bone marrow mesenchymal stem cells (BMSCs). All the composite scaffolds have a complex porous structure with proper pore sizes and porosities. Physicochemical properties of the scaffolds can be significantly increased through the incorporation of β-TCP and GO nanoparticles. Alkaline phosphatase activity (ALP) and osteogenesis-related gene expression of the BMSCs are significantly enhanced in the presence of β-TCP and GO nanoparticles. Especially, β-TCP and GO nanoparticles have a synergistic effect on promoting osteogenesis. These results suggest that the β-TCP and GO enhanced SF/SPI scaffolds are promising candidates for bone tissue regeneration.

Bi-layer silk fibroin skeleton and bladder acellular matrix hydrogel encapsulating adipose-derived stem cells for bladder reconstruction

2021 ◽  
Author(s):  
Shuwei Xiao ◽  
Pengchao Wang ◽  
Jian Zhao ◽  
Zhengyun Ling ◽  
Ziyan An ◽  
...  
Keyword(s):  
Stem Cells ◽  
Silk Fibroin ◽  
Bladder Augmentation ◽  
Adipose Derived Stem Cells ◽  
Acellular Matrix ◽  
Bladder Reconstruction

The scaffold, constructed by the bi-layer silk fibroin skeleton (BSFS) and the bladder acellular matrix hydrogel (BAMH) encapsulated with the adipose-derived stem cells (ASCs), was performed for the bladder augmentation...

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