Natural resin acid –enriched composition as a modulator of intestinal microbiota and performance enhancer in broiler chicken

2015 ◽  
Vol 3 ◽  
Author(s):  
H. Kettunen ◽  
J. Vuorenmaa ◽  
T. Rinttilä ◽  
H. Grönberg ◽  
E. Valkonen ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Intestinal Microbiota ◽  
Pure Culture ◽  
Broiler Chickens ◽  
Broiler Chicken ◽  
Resin Acid ◽  
Necrotic Enteritis ◽  
Dependent Manner ◽  
Natural Resin ◽  
Enriched Composition

SummaryResin acids extracted from coniferous trees are known for their antimicrobial and antifungal effects. This trial investigated the effect of a natural resin acid-enriched composition (RAC) on the gastrointestinal microbiota and productive performance of broiler chicken. The results demonstrated that at or above 5 mg/l, RAC prevented the growth of a pure culture ofClostridium perfringens, a causative agent of necrotic enteritis in poultry. Next, the effects of RAC on the microbial community were studied in a fermentation model with both the microbial inoculum and substrate for the microbes isolated from the ileum of broiler chickens. RAC was included at 0, 0.1 and 1 g/kg digesta, and supplementation decreased the relative proportion of lactic acid and increased that of acetic acid produced during the fermentation in a dose-dependent manner. At 1 g/kg inclusion, RAC decreased the density of lactobacilli. The final part of the experiment investigated the influence of RAC on the performance and intestinal microbiota of necrotic enteritis (NE)-challenged broiler chickens. A wheat and soy -based diet was supplemented with RAC at 0, 0.5, 1 and 3 g/kg. The chickens were challenged withEimeria maximaoocysts on day 11, and a pure culture ofC. perfringenson day 14. On day 17, the final day of the trial, RAC inclusion at 1 and 3 g/kg of feed significantly increased body weight. At 3 g/kg RAC numerically decreased the daily mortality seen during the challenge period. In the ileum, RAC at 1 g/kg reduced the NE-associated peak of microbial lactic acid production. Overall, the data suggested that the dietary ingredient RAC has the potential to act as a performance-enhancer and microbial modulator in broiler chickens.

scholarly journals Bacillus subtilis DSM 32315 Supplementation Attenuates the Effects of Clostridium perfringens Challenge on the Growth Performance and Intestinal Microbiota of Broiler Chickens

2019 ◽  
Vol 7 (3) ◽  
pp. 71 ◽  
Author(s):  
Cristiano Bortoluzzi ◽  
Bruno Serpa Vieira ◽  
Juliano Cesar de Paula Dorigam ◽  
Anita Menconi ◽  
Adebayo Sokale ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Clostridium Perfringens ◽  
Intestinal Microbiota ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
Dietary Supplementation ◽  
Necrotic Enteritis ◽  
Feed Conversion ◽  
Negative Effects ◽  
Infected Control

The objective of this study was to evaluate the effects of the dietary supplementation of Bacillus subtilis DSM 32315 (probiotic) on the performance and intestinal microbiota of broiler chickens infected with Clostridium perfringens (CP). One-day-old broiler chickens were assigned to 3 treatments with 8 replicate pens (50 birds/pen). The treatments were: non-infected control; infected control; and infected supplemented with probiotic (1 × 106 CFU/g of feed). On day of hatch, all birds were sprayed with a coccidia vaccine based on the manufacturer recommended dosage. On d 18–20 the infected birds were inoculated with CP via feed. Necrotic enteritis (NE) lesion score was performed on d 21. Digestive tract of 2 birds/pen was collected on d 21 to analyze the ileal and cecal microbiota by 16S rRNA sequencing. Performance was evaluated on d 28 and 42. On d 21, probiotic supplementation reduced (p < 0.001) the severity of NE related lesion versus infected control birds. On d 28, feed efficiency was improved (p < 0.001) in birds supplemented with probiotic versus infected control birds. On d 42, body weight gain (BW gain) and feed conversion ratio (FCR) were improved (p < 0.001) in probiotic supplemented birds versus infected control birds. The diversity, composition and predictive function of the intestinal microbial digesta changed with the infection but the supplementation of probiotic reduced these variations. Therefore, dietary supplementation of Bacillus subtilis DSM 32315 was beneficial in attenuating the negative effects of CP challenge on the performance and intestinal microbiota of broilers chickens.

scholarly journals The Role of Arginine in Disease Prevention, Gut Microbiota Modulation, Growth Performance and the Immune System of Broiler Chicken – A Review

2020 ◽  
Vol 20 (2) ◽  
pp. 325-341
Author(s):  
Harry A. Aguzey ◽  
Zhenhua Gao ◽  
Wu Haohao ◽  
Cheng Guilan ◽  
Wu Zhengmin ◽  
...  
Keyword(s):  
Immune System ◽  
Disease Prevention ◽  
Broiler Chickens ◽  
Broiler Chicken ◽  
Building Blocks ◽  
The Body ◽  
Necrotic Enteritis ◽  
Poultry Production ◽  
Growth Promoters ◽  
Immune System Modulation

AbstractThe effect of dietary arginine on disease prevention, immune system modulation, the gut micro-biota composition and growth of broiler chicken was reviewed. The main aim of poultry production is the maximization of profit at the least possible cost. This objective can mainly be achieved by ensuring that there is no interference in growth or disease outbreak and by feeding chicken with the best possible level of nutrients. With the ban on antibiotic growth promoters, attention is shifted towards other nutrition methods to prevent diseases and promote growth. More attention is therefore given to protein diets in animal nutrition due to their importance as essential part of active biological compounds in the body, assisting in the breakdown of body tissue and helping in the physiological processes of the animal. Arginine plays important function in serving as building blocks of proteins and polypeptides. It performs other roles during the regulation of important biochemical functions such as maintenance, growth, reproduction and immunity. Arginine cannot be synthesized by the body so it has to be supplemented in the diet. When arginine is supplemented above the recommended level, the gut mucosa is protected, immunosuppression is alleviated, diseases like necrotic enteritis, infectious bursal disease and coccidiosis in broiler chickens are prevented. There is an improvement in growth resulting from the increase in intestinal absorption, barrier function and microbiota composition.

Dietary resin acid composition as a performance enhancer for broiler chickens

2017 ◽  
Vol 5 ◽  
Author(s):  
H. Kettunen ◽  
E. van Eerden ◽  
K. Lipiński ◽  
T. Rinttilä ◽  
E. Valkonen ◽  
...  
Keyword(s):  
Weight Gain ◽  
Acid Composition ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
Bacterial Species ◽  
Resin Acid ◽  
The Body ◽  
Necrotic Enteritis ◽  
Feed Conversion

SummaryResin acid composition (RAC) has previously been shown to inhibit the growth of the Gram-positive bacterial species Clostridium perfringens in vitro and to modulate the ileal microbiota of broiler chickens. The following trials examined the effect of RAC on broiler chickens in two experiments. In experiment 1, 1400 one-day-old Ross 308 broilers were divided into two coccidiostat treatments: chemical (CC) and ionophore (IC), which were further divided into two RAC dosages: 0 and 0.5 g/kg. All diets were supplemented with xylanase, β-glucanase and phytase feed enzymes. The birds were raised in a commercial-type environment without additional microbial challenge during the 42-day trial. RAC improved the body weight gain by 3.3% and feed conversion ratio by 5.7% with CC, and improved footpad lesion scores with IC but had no effect on the litter quality. Experiment 2 was a 35-day subclinical necrotic enteritis (NE) challenge trial with 510 male Ross 308 chickens. The dietary treatments included a non-challenged, non-supplemented control and four NE challenged treatments with dietary RAC supplementation at 0, 1, 2, and 3 g/kg. The birds were challenged with Eimeria maxima on day nine and C. perfringens on day 14. While RAC at 1 g/kg significantly increased bird weight gain during the challenge, it did not affect the microbial or short chain fatty acid (SCFA) profiles. In contrast, RAC at 3 g/kg reduced the abundance of the Lactobacillus group and tended to reduce the abundance of genus Bifidobacterium and the total numbers of eubacteria. These experiments suggest that dietary RAC at a moderate dose positively affected broiler performance. However, changes in caecal microbiota populations may not have influenced the observed performance effects of RAC.

scholarly journals UTILIZATION PROBIOTIC LACTIC ACID BACTERIA FISH FROM WASTE FAECES ON LDL LEVEL BLOOD BROILER CHICKENS STRAIN LOHMANN

2017 ◽  
Vol 5 (1) ◽  
pp. 51 ◽  
Author(s):  
Astuti Astuti ◽  
Siti Umniyati ◽  
Anna Rakhmawati ◽  
Evy Yulianti
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Cell Count ◽  
Broiler Chickens ◽  
Broiler Chicken ◽  
Freeze Drying ◽  
Animal Husbandry ◽  
Control Treatment ◽  
Probiotic Lactic Acid Bacteria ◽  
Chicken Blood

Abstract This study aims to determine the effect of lactic acid bacteria supplement from sewge force feeding fish to the LDL content of broiler chicken blood. The research samples are 40 of 1 day old broiler chicken from PT. Multi Breeder Adiram, which were divided into 4 treatments, were taken randomly and maintined for 42 days. Data were analyzed by analysis of variance using a completely randomized design unidirectional pattern, followed by a test of Duncan's Multiple Range Test (DMRT).Probiotic Lactic Acid Bacteria Isolates treatment (BAL) were used in this study is the bacterium Streptococcus thermopillus in the form of freeze drying from the Laboratory of Nutritional Biochemistry, Faculty of Animal Husbandry, UGM. I as a control treatment (without BAL) Treatment II BAL cell count was 106 CFU / ml., The third treatment is the number of BAL Cells are 107CFU / ml. , IV treatment BAL cell count was 108 CFU /ml. The results showed that there is no significant effect of BAL isolate addition on the LDL level of the broiler chicken blood. Keywords: lactic acid bacteria, LDL, freeze drying

scholarly journals Genetic Diversity of Clostridium perfringens Strains Isolated from Broiler Chickens Revealed by PFGE Analysis in China and Pakistan

2021 ◽  
Vol 41 (01) ◽  
pp. 85-91
Author(s):  
Muhammad Umar Zafar Khan
Keyword(s):  
Clostridium Perfringens ◽  
Broiler Chickens ◽  
Broiler Chicken ◽  
Control Strategies ◽  
Type A ◽  
Necrotic Enteritis ◽  
Pfge Profile ◽  
Pfge Analysis ◽  
Lack Of Information ◽  
Beta 2

Clostridium perfringens (C. perfringens) is widely distributed in broiler chickens causing clinical and subclinical enteritis and is especially known for causing necrotic enteritis (NE). There are numerous reports of NE outbreaks in Pakistan as well as China but there is a lack of information related to PFGE profile from both the countries. To close this gap, we designed this study and obtained samples from broiler chicken farms located in 3 different regions of Pakistan and 4 different regions of China. A total of 79 fecal swabs (Pakistan=29; China=50) were collected and grown on FTA media. Further, isolates were grown on TSE agar and black colonies were selected for DNA extraction. All 79 isolates were tested for toxin profiles by PCR (α-gene; beta-2; netB gene) and PFGE profiling (pulsotypes analysis). Toxinotyping results revealed that all the isolates (n=50) from China were type A (α-toxin positive) while 23 and 6 isolates (n=29) from Pakistan were type A (α-toxin positive) and type G (α-toxin, NetB positive), respectively. Toxinotyping revealed α-toxin is highly prevalent in both the countries while from Pakistani isolates, NetB toxin was also detected. PFGE discriminated 79 isolates into 45 different PFGE patterns (pulsotypes). The analysis further showed different pulsotypes originating from China and Pakistan and isolates were subtyped by SmaI. The results showed high genetic polymorphism in C. perfringens even within the same strain. These preliminary findings of genetic variations will further help to design control strategies

scholarly journals EFISIENSI PAKAN DAN JUMLAH BAKTERI ASAM LAKTAT USUS AYAM BROILER YANG DIBERI CAIRAN SAUERKRAUT DALAM AIR MINUM

2017 ◽  
Vol 10 (01) ◽  
Author(s):  
H.Nur Fauziyah Hasanah ◽  
Tatang Sopandi
Keyword(s):  
Drinking Water ◽  
Body Weight ◽  
Lactic Acid ◽  
Weight Gain ◽  
Lactic Acid Bacteria ◽  
Feed Efficiency ◽  
Broiler Chickens ◽  
Broiler Chicken ◽  
Body Weight Gain ◽  
Final Weight

This study aims to prove the sauerkraut liquids in the drinking water of broiler chickens to weight gain, final weight, feed consumption, feed efficiency and the amount of lactic acid bacteria intestine of broilers. A total of 24 chickens broiler DOC with an average weight of 25-35 gr/tail. Broiler chickens were randomly divided into 4 groups.. Each group was given liquids sauerkraut 0 ml/L, 0.5 ml/L, 1.0 ml/L, and 1.5 ml/L in drinking water for 35 days. The results showed the treatment liquids concentration sauerkraut 1,5 ml/L in drinking water have a significant effect (P <0,05) on body weight gain, final weight, feed intake, feed efficiency and the amount of lactic acid bacteria intestines of broiler chickens. Average of body weight gain of broilers given liquids sauerkraut 1.5 ml/L in drinking water significantly (P <0.05) higher than the weight gain of broilers concentration 0 ml/L. Average of final weight of broilers given liquids sauerkraut 1,5 ml/L in drinking water significantly (P <0.05) higher than the final weight of broilers concentration 0 ml/L. Average of consumption of broiler chicken feed given liquids sauerkraut 1,5 ml/L significantly (P <0.05) lower than the consumption of broiler chicken feed concentration of 0 ml/L. Average of FCR of broilers given liquids sauerkraut 1,5 ml/L significantly (P <0.05) lower than the FCR of broilers concentration of 0 ml/L. The average amount of lactic acid bacteria intestines of broilers given liquids sauerkraut 1.5 ml/L significantly (P <0.05) higher than the amount of lactic acid bacteria intestines of broilers concentration of 0 ml/L. On the research that use of liquids sauerkraut in drinking water 1,5 ml/L can improve feed efficiency and amount of lactic acid bacteria broiler chicken intestines of 23.5%, and 19.4%.   Keywords: Broiler chickens, sauerkraut, feed efficiency, the amount of lactic acid bacteria

Studies on the mode of action of probiotics: effects of the spore-specific dipicolinic acid on selected intestinal bacteria

2005 ◽  
Vol 143 (6) ◽  
pp. 529-535 ◽  
Author(s):  
A. JADAMUS ◽  
W. VAHJEN ◽  
O. SIMON
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Broiler Chickens ◽  
Broiler Chicken ◽  
Dipicolinic Acid ◽  
Intestinal Bacteria ◽  
E Coli ◽  
Growth Inhibiting

Bacterial growth inhibiting effects of dipicolinic acid (DPA), which occurs in spores of probiotic Bacillus spp. strains, was studied with a range of Lactobacillus spp. (13), Enterococcus spp. (14), and E. coli strains (8) in vitro. In vivo effects were studied in a broiler chicken nutrition trial and DPA supplemented feed (10 g/kg).In vitro studies showed that DPA inhibited growth of most Lactobacillus spp. strains to a larger extent than E. coli strains. Enterococci were generally influenced less by DPA. However, in each group of bacteria some strains were less inhibited by DPA than others. Intestinal lactic acid bacteria in the jejunum and ileum showed a trend (P[les ]0·1) for lower counts in birds fed the DPA-supplemented feed on day 14 and 21. Counts of enterobacteria were not significantly influenced by DPA. No differences in colony counts were observed in 28-day-old animals. Digesta pH was not statistically reduced, but a lower crop pH was recorded throughout the nutrition trial.The study showed that dipicolinic acid, present in probiotic Bacillus spp. spores, is inhibitory for in vitro growth of most lactobacilli and enterobacteria, while growth of enterococci was less inhibited. Intestinal colony counts in young broiler chickens fed a DPA-supplemented diet in part mirrored in vitro results.

scholarly journals Feed Supplementation with Encapsulated Indigenous Probiotic Lactic Acid Bacteria Increased Broiler Chicken Performance

2019 ◽  
Vol 36 (1) ◽  
pp. 29-34
Author(s):  
Monica Sonia Indri Pradipta ◽  
Sri Harimurti ◽  
Widodo Widodo
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Spray Drying ◽  
Broiler Chickens ◽  
Broiler Chicken ◽  
Bacterial Species ◽  
Viable Cell Number ◽  
Probiotic Supplementation ◽  
Feed Supplementation ◽  
Probiotic Lactic Acid Bacteria

The oral application of probiotics in the poultry industry is time-consuming and laborious. Therefore, using a powdered probiotic supplement that can easily mix with feed is important. We investigated the effect of spray drying encapsulation on the viability of indigenous probiotic lactic acid bacteria during production and storage and evaluated broiler chicken performance after providing the supplement. Encapsulated probiotics exhibited >80% survival rates after spray drying. All bacterial species maintained up to 80% cell viability rates after exposure to 80–85°C temperatures for 15 or 30 s. The viable cell number of all encapsulated bacteria decreased over seven weeks of storage. The supplement was mixed with feed at concentrations 0.5 (T1), 1.0 (T2), and 1.5 (T3) g/kg feed and administered to 48 one-day-old Lohmann broiler chickens for 21 days; a T0 group was raised without probiotic supplementation. Probiotic supplementation affected body weight gain, live weight, and feed conversion ratio. The cecum length and duodenum and cecum weights significantly differed among the treatment groups. Probiotic supplementation was associated with improved villus development in the intestinal epithelium compared with that of the control. Thus, feed supplementation with indigenous probiotic powder stimulates intestinal epithelial proliferation in broiler chickens during the starter phase, improving their performance.

Kualitas Fisik Daging Ayam Broiler yang Diberi Minyak Buah Merah dalam Pakan Komersial

2019 ◽  
Vol 6 (1) ◽  
pp. 20
Author(s):  
Abdul Rahman Ollong ◽  
Rizki Arizona ◽  
Rusli Badaruddin
Keyword(s):  
Moisture Content ◽  
Broiler Chickens ◽  
Broiler Chicken ◽  
Ph Value ◽  
Cooking Loss ◽  
Water Holding Capacity ◽  
Broiler Meat ◽  
Fruit Oil ◽  
Water Holding ◽  
Range Test

ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh penambahan minyak buah merah (MBM) pada pakan terhadap kualitas fisik daging ayam broiler. Seratus ekor ayam broiler umur sehari (DOC) ditempatkan pada lima kelompok perlakuan pakan yang berbeda, yaitu: P1 (pakan kontrol/tanpa penambahan minyak), P2 (2% MBM), P3 (4% MBM), P4 (6% MBM) dan P5 (6% Minyak kelapa sawit). Setiap kelompok perlakuan terdiri dari empat ulangan masing-masing dengan lima ekor. Ayam broiler dipelihara selama 35 hari. Rancangan yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan analisis variansi pola searah dan diuji lanjut dengan Duncan’s New Multiple Range Test (DMRT). Hasil penelitian menunjukkan bahwa semua variable yang diamati menunjukkan adanya pengaruh nyata (P<0,05) terhadap perlakuan yang diberikan. Dari hasil penelitian ini dapat disimpulkan bahwa penggunaan minyak buah merah (MBM) dalam pakan mampu memberikan pengaruh terhadap pH Daging, Daya Ikat Air (DIA), susut masak dan keempukan daging ayam broiler.Kata kunci : daging ayam broiler, daya ikat air, keempukan daging, pH daging, susut masakABSTRACT The experiment was conducted to study the effect of red fruit oil (RFO) onphysical quality  of broiler chicken. One hundred day old chicken (DOC) were placed in four groups of different treatments, of from levels of RFO (P1 (diet without addition of RFO), P2 (2% RFO), P3 (4% RFO) and P4 (6% RFO) and P5 (6% Palm oil)). The treatment group consisted of fivereplications with five birds each. Broiler chickens were reared for 35 days. Statistical analysis used Completely Randomized Design (CRD) and followed by Duncan’s New Multiple Range Test (DMRT). The results showed that the pH value, moisture content, water holding capacity, and cooking loss was significant differences. It could be concluded that the addition of red fruit oil in the diet give effect  of broiler chicken meat.Keywords: broiler meat, cooking loss, moisture content, pH value, water holding capacity

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