scholarly journals Antibiotic-resistant bacteria on personal devices in hospital intensive care units: Molecular approaches to quantifying and describing changes in the bacterial community of personal mobile devices

2019 ◽  
Vol 40 (6) ◽  
pp. 717-720 ◽  
Author(s):  
Savannah J. Volkoff ◽  
Alexander W. McCumber ◽  
Deverick J. Anderson ◽  
Claudia K. Gunsch
Keyword(s):  
Antibiotic Resistance ◽  
Intensive Care ◽  
Bacterial Community ◽  
Mobile Devices ◽  
Intensive Care Units ◽  
Resistance Genes ◽  
Bacterial Community Composition ◽  
Antibiotic Resistance Genes ◽  
Resistant Bacteria ◽  
Personal Mobile Devices

AbstractBacterial community composition and presence of antibiotic resistance genes (mecA,tetK, andvanA) on personal mobile devices (PMDs) of nurses in intensive care units (ICUs) were evaluated. Antibiotic resistance genes on PMDs decreased at the end of the shift, and a several microbial genera changed.

scholarly journals Dissemination of antibiotic resistance genes associated with the sporobiota in sediments impacted by wastewater

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4989 ◽  
Author(s):  
Christophe Paul ◽  
Zhanna Bayrychenko ◽  
Thomas Junier ◽  
Sevasti Filippidou ◽  
Karin Beck ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Bacterial Community ◽  
Resistance Genes ◽  
Aquatic Ecosystems ◽  
Human Microbiome ◽  
Antibiotic Resistance Genes ◽  
Treated Wastewater ◽  
Resistant Bacteria ◽  
Term Survival ◽  
High Dispersal

Aquatic ecosystems serve as a dissemination pathway and a reservoir of both antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARG). In this study, we investigate the role of the bacterial sporobiota to act as a vector for ARG dispersal in aquatic ecosystems. The sporobiota was operationally defined as the resilient fraction of the bacterial community withstanding a harsh extraction treatment eliminating the easily lysed fraction of the total bacterial community. The sporobiota has been identified as a critical component of the human microbiome, and therefore potentially a key element in the dissemination of ARG in human-impacted environments. A region of Lake Geneva in which the accumulation of ARG in the sediments has been previously linked to the deposition of treated wastewater was selected to investigate the dissemination oftet(W) andsul1, two genes conferring resistance to tetracycline and sulfonamide, respectively. Analysis of the abundance of these ARG within the sporobiome (collection of genes of the sporobiota) and correlation with community composition and environmental parameters demonstrated that ARG can spread across the environment with the sporobiota being the dispersal vector. A highly abundant OTU affiliated with the genusClostridiumwas identified as a potential specific vector for the dissemination oftet(W), due to a strong correlation withtet(W) frequency (ARG copy numbers/ng DNA). The high dispersal rate, long-term survival, and potential reactivation of the sporobiota constitute a serious concern in terms of dissemination and persistence of ARG in the environment.

scholarly journals Fate of Antibiotic Resistance Genes in Abandoned Swine Feedlots in China: Seasonal Variation

2021 ◽  
Author(s):  
Na Li ◽  
Jianguo Chen ◽  
Chong Liu ◽  
Binxu Li ◽  
Changxiong Zhu ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Seasonal Variation ◽  
Bacterial Community ◽  
Community Composition ◽  
Resistance Genes ◽  
High Throughput Sequencing ◽  
Bacterial Community Composition ◽  
Antibiotic Resistance Genes ◽  
Rrna Gene ◽  
Variation Patterns

Abstract Background: Environmental hygiene concerns are needed to be settled before the reuse of abandoned swine feedlot sites. However, few researchers have focused on the fate of antibiotic resistance genes (ARGs) in soil microbiota around abandoned swine feedlots. In this study, we examined the seasonal alterations of ARGs and bacterial community composition in soil using quantitative PCR and high-throughput sequencing of 16S rRNA gene. Results: The seasonal variation patterns were different for different ARG subtypes and soil sampling sites. The bacterial community composition at the genus level generally showed no significant alteration from winter to summer. Moreover, the co-occurrence network suggested that the bacterial genera host range of ARGs was broader in the summer than in the winter. Conclusion: This study offers further data on ARG transfer risk in soil, emphasizing the necessity of continuous concern before reuse of abandoned feedlots.

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