scholarly journals Anthocyanin-rich purple potato flake extract has antioxidant capacity and improves antioxidant potential in rats

2006 ◽  
Vol 96 (6) ◽  
pp. 1125-1134 ◽  
Author(s):  
Kyu-Ho Han ◽  
Mitsuo Sekikawa ◽  
Ken-ichiro Shimada ◽  
Makoto Hashimoto ◽  
Naoto Hashimoto ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Control Group ◽  
Acid Oxidation ◽  
Mrna Levels ◽  
Scavenging Activity ◽  
Potato Flake ◽  
Linoleic Acid Oxidation ◽  
Potato Flakes

Anthocyanins from various vegetables and fruits have antioxidant activities, however, the bioactivities of coloured potato anthocyanins are not well studied. We examined the antioxidant capacities of pigmented fractions from purple potato flakes in vitro, and the antioxidant potentials of purple potato flakes in vivo. 1,1-Diphenyl-2-picrylhydrazyl radical scavenging activity of the pigmented fraction from Hokkai no. 92 (H92) potato flakes was higher than that from Kitamurasaki (KM) potato flakes. Extracts equivalent to 600 μg pigmented fractions from KM and H92 potato flakes inhibited linoleic acid oxidation in the order trolox>H92 ≥ KM>control. Rats were fed 25 % KM or H92 potato flake diets for 4 weeks. The major anthocyanin was identified as petanin. Control rats were fed a diet with cornstarch instead of potato flakes for 4 weeks. The serum antioxidant potential level in the H92 group was significantly higher than that in the control group. The degree of hepatic lipid peroxidation in the H92 group was significantly lower than that in the control group. Hepatic Cu/Zn-superoxide dismutase (SOD), Mn-SOD and glutathione peroxidase (GSH-Px) mRNA levels in the H92 group were significantly higher than those in the control group. Similar significant differences in Cu/Zn-SOD and Mn-SOD mRNA levels between the KM and control groups were found. The present results suggest that purple potato flakes have antioxidant functions with regard to radical scavenging activity and inhibition of linoleic acid oxidation, and that they improve the antioxidant potentials in rats by enhancing hepatic Mn-SOD, Cu/Zn-SOD and GSH-Px mRNA expression.

Investigation of the Anti-cataract and Antioxidant Activities of Cnidoscolus aconitifolius Leaves Extract In vitro

2021 ◽  
pp. 28-38
Author(s):  
Henry Bulama ◽  
Daniel Dahiru ◽  
Joshua Madu
Keyword(s):  
Total Protein ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Control Group ◽  
Scavenging Activity ◽  
Aqueous Fraction ◽  
Cnidoscolus Aconitifolius

Background: Cataract is a major cause of visual impairment and blindness around the world. This study evaluated the in vitro antioxidant and anti-cataract activities of Cnidoscolus aconitifolius leaves extract and fractions. Antioxidant activities were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azinobis (3-ethylbenzothiozoline-6-sulfonic acid) (ABTS), total reducing power, and hydrogen peroxide scavenging assays. Anti-cataract potential was evaluated in vitro using goat lenses divided into eight groups of different treatments and incubated in artificial aqueous humor at 37 °C for 72 hours. Glucose-induced opacity in the lenses was observed and biochemical indices quantified (catalase, Malondialdehyde (MDA) and total protein in the lens homogenate). Results: The crude extract and its fractions possess substantial antioxidant activities. The aqueous fraction exhibited the best DPPH radical scavenging activity (IC50 value 78.599 µg/ml); while the dichloromethane fraction exhibited the highest ABTS radical scavenging activity with IC50 66.68 µg/ml. The anti-cataract evaluation of crude and fractions at 250 μg/ml showed a significant increase (p<0.05) in the total protein and catalase activity compared to the cataract control group. The malonaldehyde level decreased significantly (p<0.05) in all the treated groups.

scholarly journals LED lighting affected the growth and metabolism of eggplant and tomato transplants in a greenhouse

2020 ◽  
Vol 47 (No. 3) ◽  
pp. 150-157
Author(s):  
Renata Wojciechowska ◽  
Anna Kołton ◽  
Olga Długosz-Grochowska ◽  
Edward Kunicki ◽  
Katarzyna Mrowiec ◽  
...  
Keyword(s):  
Radical Scavenging Activity ◽  
Soluble Sugars ◽  
Radical Scavenging ◽  
Solanum Melongena ◽  
Green Light ◽  
Control Group ◽  
Total Phenol Content ◽  
Scavenging Activity ◽  
Led Lighting ◽  
Led Light

Light-emitting diodes (LEDs) were used for the spring greenhouse cultivation of eggplant (Solanum melongena L.) ‘Milar F1’ and tomato (S. lycopersicon L.) ‘Benito F1’ transplants. Seedlings were grown under natural light conditions with the supplemental LED light. A 16-h photoperiod provided plants with a DLI of 12.6 (eggplant) and 9.6 (tomato) mol m2/day. Four supplemental light spectra were tested: L1 (90% red + 10% blue); L2 (80% red + 20% blue); L3 (43% red + 42% blue+15% green) and L4 (56% red + 26% blue + 15% green + 3% UV-A). The PPFD in each LED light treatment was 150 ± 20 µmol/m2·s. Compared to the control plants (without LED lighting), the eggplant transplants had about a 25% larger leaf area and a higher level of total phenol content as well as a higher antiradical scavenging activity under the L1 spectrum. The favourable spectrum for the tomato transplants consisted of red to blue in a ratio of 1 : 1 mixed with a green light (L3) – the leaves were characterised by a higher content of dry matter, soluble sugars, photosynthetic pigments and total phenols; also the radical scavenging activity increased in comparison to the control group. It was shown that the supplemental irradiation of transplants was economically acceptable. 

Effect of mulberry silage supplementation during late fattening stage of Hanwoo (Bos taurus coreanae) steer on antioxidative enzyme activity within the longissimus muscle

2012 ◽  
Vol 52 (4) ◽  
pp. 240 ◽  
Author(s):  
S. H. Cheong ◽  
K. H. Kim ◽  
B. T. Jeon ◽  
P. J. Park ◽  
I. H. Hwang ◽  
...  
Keyword(s):  
Bos Taurus ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Control Group ◽  
Dependent Manner ◽  
Scavenging Activity ◽  
Glutathione S Transferase ◽  
Oxidative Stress Biomarkers ◽  
Fresh Matter ◽  
Feeding Management

This study was conducted to determine the possible application of mulberry silage as a functional feed in the feeding management of Hanwoo, Korean native cattle, for high quality beef production. Twenty Hanwoo steers in the last stage of fattening were randomly assigned to two groups with (MSS) or without (Control) supplemental mulberry silage. The Control group was fed on a standard total mixed commercial ration, while the MSS group was fed on a total mixed commercial ration formulated with mulberry silage at the rate of 10%, fresh matter basis, for 30 days. The mulberry silage contained active ingredients such as 1-deoxynojirimycin, γ-aminobutyric acid, and phenol compounds. In the present study, the oxidative stress biomarkers such as glutathione, superoxide dismutase, glutathione peroxidase, catalase, and glutathione-S-transferase were improved in the MSS group compared with the Control group. Electron spin resonance assays showed that 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity was significantly elevated by 75.5% in the MSS group (P < 0.001). Moreover, the MSS group exhibited stronger scavenging activity against hydroxyl radicals, superoxide radicals, and alkyl radicals, and the radical scavenging activity occurred in a dose-dependent manner. Findings from the present study suggest that mulberry silage may be useful as a functional feed by improving the antioxidant activity against various reactive radicals for beef cattle.

Effects of Thymus revolutus Célak essential oil and its two major components on Hep G2 cells membrane

Biologia ◽  
2013 ◽  
Vol 68 (1) ◽  
Author(s):  
Ayse Erdogan ◽  
Aysun Ozkan
Keyword(s):  
Linoleic Acid ◽  
Essential Oil ◽  
Radical Scavenging Activity ◽  
Membrane Damage ◽  
Radical Scavenging ◽  
Acid Oxidation ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
G2 Cells ◽  
Main Components

AbstractIn this study pro/anti-oxidant activities of Thymus revolutus Célak (T. revolutus C.) essential oil and its two main components on Hep G2 cells were determined. Twelve components were identified in T. revolutus C. essential oil by GC and GC-MS and the main components of the oil were cymene (32.57%) and γ-terpinene (17.18%). The essential oil showed more cytotoxicity than its two main components on Hep G2 after 24, 48 and 72 hours incubations. Membrane damage effects of the essential oil and its two main components on Hep G2 cells were increased by incubation with IC10, IC50 and IC70 concentrations. Lower essential oil (<IC50) concentrations had cytoprotective and membrane protective effects against H2O2. The free radical scavenging activity of the essential oil of T. revolutus C. (EC50 = 250±0.2 μg/mL) was superior to its two main components (neither not active). Oxidation of linoleic acid was effectively inhibited by T. revolutus C. (72.8%), while inhibition values of linoleic acid oxidation were calculated as 23.4.% and 26.7% for cymene and Γ-terpinene, respectively. These findings suggest that essential oils and its two main components had both pro-oxidant and protective (antioxidant) effects and they exhibited those effects depending on concentration.

scholarly journals Hypotensive effect of Cichorium intybus extract in rats

2021 ◽  
Vol 10 (2) ◽  
pp. 257-261
Author(s):  
Mehrnoosh Sedighi ◽  
Mostafa Cheraghi ◽  
Mahdieh Faghihi ◽  
Mohammad Rahimi-Madiseh ◽  
Ali Asghar Kiani ◽  
...  
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Cichorium Intybus ◽  
Free Radical Scavenging Activity ◽  
Control Group ◽  
Antioxidant Compounds ◽  
Scavenging Activity ◽  
Phenolic And Flavonoid

Introduction: Oxidative stress is involved in many diseases, including hypertension, kidney failure, and heart disease. This study aimed to evaluate the effect of hydroalcoholic Cichorium intybus extract on blood pressure in rats. Antioxidant activity, phenolic and flavonoid contents of the plant extract were also evaluated. Methods: In this study, 32 male Wistar rats weighing 250-300 g were divided into four groups of eight each. Animals in the control group were administered with normal saline and in the C. intybus groups with extract at 25, 50, and 100 mg/kg for two weeks. Then, the homodynamic parameters were examined by the Power lab. The phenolic and flavonoid contents were also evaluated by a spectrophotometer and the rate of free radical scavenging activity was measured by the diphenyl-1-picyryl-hydrazyl (DPPH) free radical method. Results: The free radical scavenging activity of C. intybus extract was obtained 47.85% of DPPH, and flavonoid and phenolic contents were 8.21 and 27.19 mg/g of dry extract, respectively. Meanwhile, median (MAP), systolic (SAP) and diastolic arterial pressure (DAP) significantly decreased in the 50 mg/kg extract-treated group compared to the control and 200 mg/kg extract-treated groups. Conclusion: Ethanol extract of C. intybus plays a protective role against hypertension, which, in part, might be due to antioxidant compounds of the plant against free radicals.

scholarly journals Free radical scavenging activity of conjugated linoleic acid as single or mixed isomers

2011 ◽  
Vol 50 (6) ◽  
pp. 712-719 ◽  
Author(s):  
Yassir Mohammed Ali ◽  
Arifah Abdul Kadir ◽  
Zuraini Ahmad ◽  
Halimatun Yaakub ◽  
Zainul Amiruddin Zakaria ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Free Radical ◽  
Conjugated Linoleic Acid ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity

scholarly journals Antioxidant activities of pomegranate peel methanolic and water extracts by in vitro methods

2021 ◽  
Vol 4 (1) ◽  
pp. 1-6
Author(s):  
Filiz Ucan Turkmen ◽  
Fatma Esen Sarigullu Onalan ◽  
Hatice Aysun Mercimek Takci
Keyword(s):  
Methanol Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Punica Granatum ◽  
Acid Oxidation ◽  
Linoleic Acid Oxidation ◽  
Trolox Equivalent

In this study, the antioxidant activities of pomegranate (Punica granatum) peel extracts were investigated using different antioxidant activity methods. The antioxidant activities of extracts prepared at different concentrations (100-1000 μg/ml) were compared with α-tocopherol, ascorbic acid, BHT, and BHA standards. The methanol extract of peels had the highest yield (45.5%). Chelating activities (Fe2+) of methanol extract at 100-1000 μg/ml concentrations ranged between 16.63 and 46.45%. However, the chelating activities of all test extracts were lower than the standards. The reducing power of all extracts increased concentration-dependent. DPPH˙˙ radical scavenging activity of methanol extract varied in the range of 18.53 to 88.75%. The highest percentage inhibition of linoleic acid oxidation was found in 250 μg/ml concentrations. Trolox equivalent (TEs) antioxidant capacity values of methanol extract ranged from 3.09 to 15.27 µM TEs 10 g-1. It was determined that methanol extract showed a dose-dependent inhibition of the antioxidant activities.

scholarly journals DERIVATIZATION OF CHLOROPHYLL FROM PANDAN (Pandanus amaryllifolius Roxb.) LEAVES AND THEIR ANTIOXIDANT ACTIVITY

2020 ◽  
Vol 17 (36) ◽  
pp. 1110-1126
Author(s):  
Chatarina Lilis SURYANI ◽  
Tutik Dwi WAHYUNINGSIH ◽  
SUPRIYADI A. ◽  
Umar SANTOSO
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Chemical Structure ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
The Other ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Metal Chelating ◽  
Pandanus Amaryllifolius

Chlorophyll is a natural coloring agent that has antioxidant activity. During the extraction and food processing process, chlorophyll is easily degraded and derivatized. The derivatization process results in changes in the chemical structure of chlorophyll which can result in a change in color and its antioxidant activity. The other extracted minor compounds can affect its antioxidant activity. This study aimed to identify the chemical structure changes of chlorophyll extracted from pandan (Pandanus amaryllifolius Roxb) leaves as affected by the derivatization process and evaluate the antioxidant activity of the extracts and fractions of chlorophyll and its derivatives. Chlorophyll was extracted from pandan leaves with acetone, derivatized, and then purified by fractionation using column chromatography. Chlorophyll extract and its derivatives were analyzed for total carotenoid, total phenolic, and flavonoid contents. The antioxidant activity of extract and fraction was measured by the inhibition of peroxidation of linoleic acid, radical scavenging activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing/ antioxidant power (FRAP) ability, and metal chelating assay. Based on the FTIR and MS/MS spectra, it was observed that the first derivatives were pheophytin and chlorophyllide, while the second derivative was pheophorbide. The other extracted compounds, including phenolic compounds, flavonoids, and carotenoids, might enhance the antioxidant activity of the chlorophyll derivatives. The inhibition rate of linoleic acid peroxidation by chlorophyll, chlorophyllide, and pheophorbide extract was not significantly different from BHT, where pheophytin extract was lower. The radical scavenging activity of DPPH and FRAP assay showed that chlorophyll and chlorophyllide extracts exhibited higher activity, followed by pheophytin and pheophorbide. Meanwhile, the metal chelating assay showed that chlorophyllide fraction exhibited the lowest activity. Therefore, the antioxidant activity of the first derivatives of chlorophyll was higher than the second derivatives. In general, the extract of chlorophyll and its derivatives exhibited higher antioxidant activity than that of their fraction.

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