Combined bead-based multiplex detection of RNA and protein biomarkers: Implications for understanding the time course of skeletal muscle injury and repair

Methods ◽  
2019 ◽  
Vol 158 ◽  
pp. 92-96 ◽  
Author(s):  
Melody A. Gary ◽  
Elizabeth A. Tanner ◽  
Asheal A. Davis ◽  
Brian K. McFarlin
Keyword(s):  
Skeletal Muscle ◽  
Muscle Injury ◽  
Time Course ◽  
Multiplex Detection ◽  
Protein Biomarkers ◽  
Skeletal Muscle Injury ◽  
Injury And Repair

Time course of chemokine expression and leukocyte infiltration after acute skeletal muscle injury in mice

2014 ◽  
Vol 21 (3) ◽  
pp. 266-274 ◽  
Author(s):  
Jennifer Nicholas ◽  
Joachim G Voss ◽  
Joyce Tsuji ◽  
Nadia D Fulkerson ◽  
Julia Soulakova ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Injury ◽  
Time Course ◽  
Leukocyte Infiltration ◽  
Skeletal Muscle Injury ◽  
Chemokine Expression

Skeletal muscle injury and repair: A role for complement

2018 ◽  
Vol 102 ◽  
pp. 165
Author(s):  
Erin Jacobs ◽  
Carolina Ortiz ◽  
Edouard Al-Chami ◽  
Mohsen Afshar ◽  
Clinton Robbins ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Injury ◽  
Skeletal Muscle Injury ◽  
Injury And Repair

scholarly journals Annexin A1 drives macrophage skewing towards a resolving phenotype to accelerate the regeneration of muscle injury through AMPK activation

2018 ◽  
Author(s):  
Simon McArthur ◽  
Thomas Gobbetti ◽  
Gaëtan Juban ◽  
Thibaut Desgeorges ◽  
Marine Theret ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Injury ◽  
Muscle Fibres ◽  
Annexin A1 ◽  
Skeletal Muscle Injury ◽  
Cellular Processes ◽  
Reparative Process ◽  
Injury And Repair ◽  
Inflammation Resolution

SummaryUnderstanding the circuits that promote an efficient resolution of inflammation is crucial to deciphering the molecular and cellular processes required to promote tissue repair. Macrophages play a central role in the regulation of inflammation, resolution and repair/regeneration. Using a model of skeletal muscle injury and repair, herein we identify Annexin A1 (AnxA1) as the extracellular trigger of macrophage skewing towards a pro-reparative phenotype. Brought into the injured tissue initially by migrated neutrophils, and then over-expressed in infiltrating macrophages, AnxA1 activates FPR2/ALX receptors and the downstream AMPK signalling cascade leading to macrophage skewing, dampening of inflammation and regeneration of muscle fibres. Mice lacking AnxA1 in all cells or in myeloid cells only display a defect in this reparative process.In vitroexperiments recapitulated these properties, with AMPK null macrophages lacking AnxA1-mediated polarization. Collectively, these data identify the AnxA1/FPR2/AMPK axis as a novel pathway in skeletal muscle injury regeneration.

Combining single molecule counting with bead-based multiplexing to quantify biological inflammation time course following skeletal muscle injury

Methods ◽  
2019 ◽  
Vol 158 ◽  
pp. 77-80 ◽  
Author(s):  
Elizabeth A. Tanner ◽  
Melody A. Gary ◽  
Asheal A. Davis ◽  
Brian K. McFarlin
Keyword(s):  
Skeletal Muscle ◽  
Single Molecule ◽  
Muscle Injury ◽  
Time Course ◽  
Skeletal Muscle Injury

scholarly journals Mechanisms of skeletal muscle injury and repair revealed by gene expression studies in mouse models

2007 ◽  
Vol 582 (2) ◽  
pp. 825-841 ◽  
Author(s):  
Gordon L. Warren ◽  
Mukesh Summan ◽  
Xin Gao ◽  
Rebecca Chapman ◽  
Tracy Hulderman ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Mouse Models ◽  
Muscle Injury ◽  
Skeletal Muscle Injury ◽  
Expression Studies ◽  
Gene Expression Studies ◽  
Injury And Repair
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